We narrowed to 3,245 results for: zebrafish

1. pCS2+-N-PAS2-GAF-PHYB-MCherry-CAAX

   Plasmid

   #154910

   Purpose

   1-621 amino acids of Arabidopsis PHYB (Gene ID: 816394), tagged with mCherry fluorescence protein and the CAAX membrane moiety. Mammalian codon optimised.

   Depositor

   Clare Buckley, Jonathan Clarke

   Article

   Buckley et al Dev Cell. 2016 Jan 11;36(1):117-26. doi:

   Insert

   Phytochrome B (PHYB Mustard Weed, Synthetic)
   

   Use

   Xenopus/avian/zebrafish

   Tags

   CAAX membrane moiety and mCherry

   Expression

   Mammalian

   Mutation

   1-621 amino acids of Arabidopsis PHYB

   Promoter

   CMV

   Available Since

   Nov. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHR_PGK_antiCD19_eZ3_Gal4VP64

   Plasmid

   #169917

   Purpose

   Expression of a synNotch receptor containing antiCD19, a zebrafish Notch3 core with an additional EGF repeat, and Gal4VP64.

   Depositor

   Zhili Rong

   Article

   Huang et al Protein Cell. 2020 Apr;11(4):299-303. do

   Insert

   antiCD19-Notch3-GL4VP64
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   Additional EGF repeat between the extracellular d…

   Promoter

   PGK

   Available Since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. rag2:MYRFL_CE_pISceI

   Plasmid

   #223045

   Purpose

   Express MYRFL gene in mesenchymal lineage of Zebrafish.

   Depositor

   Alejandro Gutierrez

   Article

   Ali et al Mol Cell. 2025 Mar 6;85(5):948-961.e11.

   Insert

   MYRFL
   

   Use

   Express the insert(gene) in mesenchymal lineage o…

   Promoter

   Rag2

   Available Since

   Feb. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. AsCas12a-2C-NLS in pCSDest

   Plasmid

   #126637

   Purpose

   Expresses AsCas12a-2C-NLS in mammalian cells

   Depositor

   Scot Wolfe

   Article

   Liu et al Nucleic Acids Res. 2019 Mar 20. pii: 540

   Insert

   AsCas12a-2C-NLS
   

   Use

   CRISPR

   Tags

   3xHuman influenza hemagglutinin (HA)-Tag

   Expression

   Mammalian

   Promoter

   CMV IE94 promoter

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCS2+-Y276H PHYB-CAAX

   Plasmid

   #154911

   Purpose

   1-621 amino acids of Arabidopsis PHYB (as in construct Addgene ID 154910) with the CAAX membrane moiety tag. Tyrosine residue 276 of PHYB was mutated to Histidine.

   Depositor

   Clare Buckley, Jonathan Clarke

   Article

   Buckley et al Dev Cell. 2016 Jan 11;36(1):117-26. doi:

   Insert

   Phytochrome B (PHYB Mustard Weed)
   

   Use

   Xenopus/avian/zebrafish

   Tags

   CAAX membrane moiety and mCherry

   Expression

   Mammalian

   Mutation

   1-621 amino acids of Arabidopsis PHYB, Tyrosine r…

   Promoter

   CMV

   Available Since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. Danio alpha A-crystallin 1kb promoter/AcGFP

   Plasmid

   #98096

   Purpose

   aA-crystallin promoter fragment in GFP plasmid to assess activity in larval embryos

   Depositor

   Mason Posner

   Article

   Posner et al PeerJ. 2017 Nov 27;5:e4093. doi: 10.7717

   Insert

   Alpha A-crystallin promoter 1 kb fragment, Danio rerio (cryaa Zebrafish)
   

   Use

   Gfp expressing

   Tags

   GFP

   Promoter

   Danio alpha A-crystallin 1 kb fragment (-1028/-1)

   Available Since

   Sept. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. ACVR1-TAAS-R206H

   Plasmid

   #206184

   Purpose

   in vitro transcription of RNA encoding human ACVR1 with mutations S190A, S192A, and R206H

   Depositor

   Mary Mullins

   Article

   Allen et al J Bone Miner Res. 2023 Jun 17. doi: 10.1

   Insert

   activin A receptor type 1 (ACVR1 Human)
   

   Use

   In vitro transcription

   Tags

   FLAG tag

   Mutation

   S190A, S192A, R206H

   Available Since

   Oct. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAG657 H2B-pFAST

   Plasmid

   #172862

   Purpose

   Expresses pFAST fused to zebrafish histone H2B in mammalian cells

   Depositor

   Arnaud Gautier

   Article

   Benaissa et al Nat Commun. 2021 Nov 30;12(1):6989. doi:

   Insert

   H2B-pFAST
   

   Tags

   Myc tag (C terminal on insert)

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. actc1b-mKate2-CDC42

   Plasmid

   #109531

   Purpose

   Muscle specific zebrafish expression of mKate2 fused to CDC42

   Depositor

   Rob Parton

   Article

   Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

   Insert

   CDC42 (cdc42 Zebrafish)
   

   Use

   Zebrafish plasmids

   Tags

   mKate2

   Available Since

   Feb. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCS2plus-mVenus1-155-I152L-GGGS

    Plasmid

    #162613

    Purpose

    Allows for transcription of improved control mVenus I152L fluorophore half for injection into zebrafish embryos for BiFC assays

    Depositor

    Marco Morsch

    Article

    Don et al Mol Neurobiol. 2021 May;58(5):2061-2074.

    Insert

    mVenus
    

    Expression

    Mammalian

    Mutation

    1-155-I152L

    Available Since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. actc1b-mKate2-CDC42-DN

    Plasmid

    #109532

    Purpose

    Muscle specific zebrafish expression of mKate2 fused to dominant negative CDC42

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    CDC42 (cdc42 Zebrafish)
    

    Use

    Zebrafish plasmids

    Tags

    mKate2

    Mutation

    DN

    Available Since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMTB2-Avi-Cerulean-RanGAP

    Plasmid

    #79878

    Purpose

    Beta-actin2 promoter driving N-term Avi-tagged protein containing Cerulean protein fused to the carboxy-terminal domain of avian Ran GTPase-activating protein 1 (RanGap1); flanked by Tol2 sequences

    Depositor

    Tatjana Sauka-Spengler

    Article

    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

    Insert

    Cerulean protein fused to the carboxy-terminal domain of avian Ran GTPase-activating protein 1 (RanGap1) (RANGAP1 Chicken)
    

    Tags

    Avi

    Expression

    Bacterial

    Promoter

    Beta-actin2

    Available Since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. FnoCas12a-2C-NLS in pCSDest

    Plasmid

    #126639

    Purpose

    Expresses FnoCas12a-2C-NLS in mammalian cells

    Depositor

    Scot Wolfe

    Article

    Liu et al Nucleic Acids Res. 2019 Mar 20. pii: 540

    Insert

    FnoCas12a-2C-NLS
    

    Use

    CRISPR

    Tags

    3xHuman influenza hemagglutinin (HA)-Tag

    Expression

    Mammalian

    Promoter

    CMV IE94 promoter

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGEM-T-Easy-NLS-Cas9m4-NLS-VP64-TaV-2a-Citrine-FRT-Kan-FRT

    Plasmid

    #119067

    Purpose

    Donor construct to amplify NLS-Cas9m4-NLS-VP64-TaV-2a-Citrine-FRT-Kan-FRT cassette for BAC recombination

    Depositor

    Tatjana Sauka-Spengler

    Article

    Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

    Insert

    NLS-Cas9m4-NLS-VP64-TaV-2a-Citrine-FRT-Kan-FRT
    

    Expression

    Bacterial

    Available Since

    Feb. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGEM-T-Easy-HA-NLS-dCas9-NLS-SID4x-TaV-2a-Citrine-FRT-Kan-FRT

    Plasmid

    #119065

    Purpose

    Donor construct to amplify HA-NLS-dCas9-NLS-SID4x-TaV-2a-Citrine-FRT-Kan-FRT cassette for BAC recombination

    Depositor

    Tatjana Sauka-Spengler

    Article

    Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

    Insert

    HA-NLS-dCas9-NLS-SID4x-TaV-2a-Citrine-FRT-Kan-FRT
    

    Tags

    HA

    Expression

    Bacterial

    Available Since

    Feb. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRM27

    Plasmid

    #163695

    Purpose

    ubb promoter driven expression of lck:mNeonGreen

    Depositor

    David Matus

    Article

    Adikes et al Elife. 2020 Dec 22;9. pii: 63265. doi: 1

    Insert

    lck:mNeonGreen (lck Zebrafish)
    

    Use

    Zebrafish expression

    Tags

    mNeonGreen

    Promoter

    ubb

    Available Since

    March 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGEM-cytoBirA-2A-mCherry-SV40pA-FKF

    Plasmid

    #79887

    Purpose

    Donor cassette containing HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), mCherry protein and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection gene

    Depositor

    Tatjana Sauka-Spengler

    Article

    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

    Insert

    HA-tagged BirA, 2A, mCherry protein and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
    

    Expression

    Bacterial

    Available Since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. actc1b-mKate2-SERCA1

    Plasmid

    #109498

    Purpose

    Muscle specific zebrafish expression of mKate2 fused to sarcoplasmic/endoplasmic calcium ATPase 1

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    SERCA1 (atp2a1 Zebrafish)
    

    Use

    Zebrafish plasmids

    Tags

    mKate2

    Mutation

    Please see Depositor Comments

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. LbCas12a-DR-GC-swap-cr in pBCSK+

    Plasmid

    #126643

    Purpose

    Cloning vector LbCas12a-DR-GC-swap-crRNA for expression in mammalian cells

    Depositor

    Scot Wolfe

    Article

    Liu et al Nucleic Acids Res. 2019 Mar 20. pii: 540

    Insert

    LbCas12a Full Length Direct Repeat GC Swap crRNA
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6 Promoter

    Available Since

    July 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCK072 p5E 147bp exorh:EGFP

    Plasmid

    #195950

    Purpose

    Gateway p5E vector with reporter

    Depositor

    Christian Mosimann

    Article

    Kemmler et al Development. 2023 Mar 28:dev.201531. doi

    Insert

    exorh minimal regulatory elements and EGFP
    

    Use

    Other

    Available Since

    Feb. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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