We narrowed to 27,062 results for: form

1. pCAG-ERT2CreERT2

   Plasmid

   #13777

   Purpose

   Mammalian expression of conditionally active form of Cre recombinase (activated in response to tamoxifen)

   Depositor

   Connie Cepko

   Article

   Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

   Insert

   ERT2-Cre-ERT2 fusion protein
   

   Use

   Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   April 20, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG-CreERT2

   Plasmid

   #14797

   Purpose

   Mammalian expression of conditionally active form of Cre recombinase (not as tightly regulated as pCAG-ERT2CreERT2)

   Depositor

   Connie Cepko

   Article

   Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

   Insert

   Cre-ERT2 fusion protein
   

   Use

   Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   April 20, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDAI-SceI

   Plasmid

   #32061

   Purpose

   Construction of unmarked gene deletions. Broad host range replicative plasmid expressing the I-SceI homing endonuclease

   Depositor

   Miguel Valvano

   Article

   Flannagan et al Environ Microbiol. 2008 Jun;10(6):1652-6

   Insert

   I-SceI nuclease
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Pdhfr

   Available since

   Oct. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSANG10-3F

   Plasmid

   #39264

   Depositor

   John McCafferty

   Article

   Martin et al BMC Biotechnol. 2006 Dec 7;6:46.

   Type

   Empty backbone

   Use

   Bacterial expression, bacterial expression of re…

   Tags

   6xHis, pelB leader, and tri-FLAG

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 30, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. HA-ISceID44A

   Plasmid

   #59424

   Purpose

   Expresses a catalytically inactive form of the endonuclease ISceI

   Depositor

   Tom Misteli

   Article

   Roukos et al Science. 2013 Aug 9;341(6146):660-4. doi

   Insert

   endonuclease ISceI mutant (I-SceI )
   

   Use

   Tags

   HA tag

   Expression

   Mammalian

   Mutation

   changed aspartate 44 to alanine (D44A)

   Promoter

   Available since

   Dec. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET24c(+)-mtEFTu

   Plasmid

   #30535

   Depositor

   Linda Spremulli

   Article

   Woriax et al Biochim Biophys Acta. 1995 Dec 27. 1264(

   Insert

   Mature bovine mitochondrial elongation factor Tu
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Mature form amino acids 44-452. Mito import seque…

   Promoter

   Available since

   Oct. 6, 2011

   Availability

   Academic Institutions and Nonprofits only

7. pSG5 E107K T Ag

   Plasmid

   #133710

   Purpose

   Large T antigen mutant form in pSG5 vector for mammalian expression.

   Depositor

   James A. DeCaprio

   Article

   Zalvide et al Mol Cell Biol. 1995 Oct . 15(10):5800-10

   Insert

   SV40gp6 (SV40gp6 SV40)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   E107K

   Promoter

   Available since

   April 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJK370

   Plasmid

   #73595

   Purpose

   Encodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) with C-terminal FLAG epitope and delta-12 fatty acid desaturase (FAD2-2) with C-terminal c-myc epitope

   Depositor

   T. J. Kappock

   Inserts

   delta-12 fatty acid acetylenase
   delta-12 fatty acid desaturase (form 2)
   

   Use

   Tags

   FLAG tag and c-myc tag

   Expression

   Yeast

   Mutation

   Silent mutation in Arg299 codon.

   Promoter

   GAL1 and GAL10

   Available since

   Feb. 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCS2-Tm2AG

   Plasmid

   #26773

   Depositor

   Shankar Srinivas

   Article

   Trichas et al BMC Biol. 2008 . 6():40.

   Insert

   TdTomato-2A-H2BGFP-SV40pA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   mutation in 2A peptide that renders it non-functi…

   Promoter

   Available since

   March 14, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. ADP1-ISx

    Bacterial Strain

    #216551

    Purpose

    Acinetobacter baylyi strain with transposons deleted from its genome

    Depositor

    Jeffrey Barrick

    Article

    Suarez et al Appl Environ Microbiol. 2017 Aug 17;83(1

    Bacterial Resistance

    None

    Available since

    June 10, 2024

    Availability

    Academic Institutions and Nonprofits only

11. DHIP1

    Bacterial Strain

    #226851

    Purpose

    Expresses HIP1 methyltransferase to methylate plasmids for delivery to cyanobacteria

    Depositor

    David Nielsen

    Article

    Kamoku et al Biotechnol Prog. 2025 Jan 23:e3518. doi:

    Bacterial Resistance

    Streptomycin

    Species

    Escherichia coli

    Available since

    Nov. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

12. ATMW1

    Bacterial Strain

    #218768

    Purpose

    E. coli strain, genotype: EcNR1 pUltraG ScW40 CCA trpS::ZeoR trpT::gentR dgalK lambdaRED::galK

    Depositor

    Abhishek Chatterjee

    Article

    Italia et al Nat Chem Biol. 2017 Apr;13(4):446-450. d

    Bacterial Resistance

    Spectinomycin (50 ug/ml), gentamycin (10 ug/ml)

    Species

    Escherichia coli

    Available since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

13. pcDNA3-myc NES-mCE NLS

    Plasmid

    #82466

    Purpose

    Expresses cytoplasmic restricted form of myc tagged mRNA capping enzyme

    Depositor

    Daniel Schoenberg

    Article

    Otsuka et al Mol Cell Biol. 2009 Apr;29(8):2155-67. d

    Insert

    NES mRNA capping enzyme (Rngtt HIV, Mouse)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBITE-CD19-CD3

    Plasmid

    #190677

    Purpose

    Encodes a modularized form of HD37-OKT3 bispecific antibody

    Depositor

    Scott McComb

    Article

    Shepherd et al PLoS One. 2023 Jun 22;18(6):e0273884. do

    Insert

    Modularized-HD37-modularized-OKT3-P2A-NeonGreen
    

    Use

    Lentiviral

    Tags

    mNeonGreen

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1 MCS-BirA(R118G)-HA

    Plasmid

    #36047

    Purpose

    To fuse your protein of interest to the N-terminus of BirA(R118G) and use in proximity-dependent biotin identification (BioID); HA tag

    Depositor

    Kyle Roux

    Article

    Roux et al J Cell Biol. 2012 Mar 12.

    Has Service

    Cloning Grade DNA

    Insert

    BirA(R118G)-HA
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    R118G (highly promiscuous form)

    Promoter

    Available since

    April 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUC57-mitoTPNOX

    Plasmid

    #87854

    Purpose

    This plasmid contains human codon optimized sequence of Lactobacillus brevis mutant of a water-forming oxidase that can be used to increase NADP+/NADPH ratio in mammalian cells.

    Depositor

    Vamsi Mootha

    Article

    Cracan et al Nat Chem Biol. 2017 Aug 7. doi: 10.1038/

    Insert

    mitoTPNOX
    

    Use

    Tags

    C-terminal FLAG tag with a linker and mitochondri…

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET28a-SnoopCatcher

    Plasmid

    #72322

    Purpose

    Bacterial expression of SnoopCatcher, an engineered protein forming a spontaneous covalent bond with its cognate peptide partner SnoopTag.

    Depositor

    Mark Howarth

    Article

    Veggiani et al Proc Natl Acad Sci U S A. 2016 Jan 19. p

    Insert

    pET28a-SnoopCatcher
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    changed G842T and D848G of S. pneumoniae adhesin …

    Promoter

    T7

    Available since

    Feb. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCX-mutSpiCee

    Plasmid

    #140903

    Purpose

    mammalian expression of a mutated form of SpiCee, that does not affect calcium signaling

    Depositor

    Xavier Nicol

    Article

    Ros et al Cell Rep. 2020 Jul 21;32(3):107934. doi:

    Insert

    mutSpiCee
    

    Use

    Tags

    mRFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Aug. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET15bpATnp

    Plasmid

    #121137

    Purpose

    This vector encode a His6-tagged proteinA-Tnp(hyperactive form) fusion protein.

    Depositor

    Keji Zhao

    Article

    Carter et al Nat Commun. 2019 Aug 20;10(1):3747. doi:

    Insert

    Protein A-Tnp
    

    Use

    Tags

    his

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. SAE1

    Plasmid

    #175241

    Purpose

    Bacterial expression and purification, combined with SAE2 to form the E1 enzyme heterodimer

    Depositor

    Michael Rosen

    Article

    Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

    Insert

    SAE1 (SAE1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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