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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 4,802 results for: pcr

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Showing: 2501 - 2520 of 4802 results

  10. GLRX3

    Plasmid
    #39036
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    GLRX3 (GLRX3 Human)
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    A PCR artifact adds a C-terminal sequence includi…
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  12. SEMA3D (mouse)

    Plasmid
    #29452
    Depositor
    Jonathan Raper
    Insert
    SEMA3D (Sema3d Mouse)
    Use
    For ish
    Mutation
    insert is not full length; contains multiple poin…
    Available Since
    Feb. 24, 2012
    Availability
    Academic Institutions and Nonprofits only

  13. pENTR-D-kcnj16

    Plasmid
    #173146
    Purpose
    This gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.
    Depositor
    GuangJun Zhang
    Article
    Silic et al Dev Dyn. 2021 Sep 23. doi: 10.1002/dvdy.
    Insert
    kcnj16a (kcnj16 Zebrafish)
    Use
    Pcr cloning vector
    Mutation
    Stop codon removed
    Promoter
    No promoter at 5 end; T7 promoter at 3 end.
    Available Since
    Sept. 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    173146-plasmid-map-sequence-id-343336

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  16. pAAV_hSyn-PdCO-EGFP-WPRE (AAV5)

    Viral Prep
    #198513-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from pAAV_hSyn-PdCO-EGFP-WPRE (#198513). In addition to the viral particles, you will also receive purified pAAV_hSyn-PdCO-EGFP-WPRE plasmid DNA. Synapsin-driven expression of the optimized opsin PdCO in frame with EGFP. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Ofer Yizhar
    Article
    Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s
    Promoter
    Syn
    Tags
    EGFP
    Available Since
    April 10, 2024
    Availability
    Academic Institutions and Nonprofits only

  20. GCGR-DuET

    Plasmid
    #213250
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    GCGR (GCGR Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213250-plasmid-map-sequence-id-430801

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Showing: 2501 - 2520 of 4802 results