We narrowed to 9,753 results for: try

1. pET51b-His-TEV-SNAP-tag

   Plasmid

   #167269

   Purpose

   Overexpression of SNAP-tag in E. coli

   Depositor

   Kai Johnsson

   Article

   Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

   Insert

   SNAP-tag
   

   Tags

   His x10

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTriEx4-Rac1-2G

   Plasmid

   #66110

   Purpose

   Second generation Rac1 FRET biosensor

   Depositor

   Olivier Pertz

   Article

   Fritz et al Dev Cell. 2015 Sep 29. pii: S1534-5807(1

   Insert

   Second generation Rac1 FRET biosensor
   

   Tags

   His-Myc

   Expression

   Bacterial, Insect, and Mamm…

   Promoter

   CMV

   Available Since

   Oct. 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUC19 PKC delta

   Plasmid

   #8427

   Depositor

   Frederic Mushinski

   Article

   Mischak et al Biochemistry 1991 Aug 13;30(32):7925-31.

   Insert

   Mouse Protein Kinase C delta (Prkcd Mouse)
   

   Expression

   Bacterial

   Available Since

   April 28, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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4. G1333 DddAtox-C–dSpCas9

   Plasmid

   #157834

   Purpose

   expresses split DddAtox-Cas9 construct in mammalian cells

   Depositor

   David Liu

   Article

   Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

   Insert

   bpNLS–G1333 DddAtox-C–dSpCas9–UGI–UGI–bpNLS
   

   Expression

   Mammalian

   Available Since

   Aug. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCDNA3_LuxSit_i_mTagBFP2

   Plasmid

   #194300

   Purpose

   Mammalian expression of the de novo luciferase, LuxSit-i

   Depositor

   David Baker, Andy Yeh

   Article

   Yeh et al Nature. 2023 Feb;614(7949):774-780. doi:

   Insert

   LuxSit-i
   

   Tags

   mTagBFP2

   Expression

   Mammalian

   Available Since

   March 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAT-WT-Myc

   Plasmid

   #177717

   Purpose

   ER protein alpha-1-antitrypsin (WT), myc tagged

   Depositor

   Erik Böttger

   Article

   Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

   Insert

   alpha-1-antitrypsin (SERPINA1 Human)
   

   Tags

   Myc

   Expression

   Bacterial and Mammalian

   Available Since

   Jan. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET22b_PstS_1

   Plasmid

   #78198

   Purpose

   Expression of single-cysteine variant of mature phosphate binding protein, PstS, as scaffold for phosphate biosensor

   Depositor

   Martin Webb

   Article

   Solscheid et al Biochemistry. 2015 Aug 18;54(32):5054-62

   Insert

   pstS (A197C)
   

   Expression

   Bacterial

   Mutation

   A197C (please see depositors comment below)

   Promoter

   T7

   Available Since

   July 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pR-mTagBFP2

   Plasmid

   #228596

   Purpose

   Mammalian expression of mTagBFP2, for MuSIC barcode construction

   Depositor

   Marc Birtwistle

   Article

   Lu et al ACS Synth Biol. 2025 May 16;14(5):1533-1

   Insert

   mTagBFP2
   

   Expression

   Mammalian

   Mutation

   We introduced some silent mutations to avoid esse…

   Promoter

   CMV

   Available Since

   Nov. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pR-mRuby3

   Plasmid

   #228609

   Purpose

   Mammalian expression of mRuby3, for MuSIC barcode construction

   Depositor

   Marc Birtwistle

   Article

   Lu et al ACS Synth Biol. 2025 May 16;14(5):1533-1

   Insert

   mRuby3
   

   Expression

   Mammalian

   Mutation

   We introduced some silent mutations to avoid esse…

   Promoter

   CMV

   Available Since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. mVenus-IQ6 WT

    Plasmid

    #198201

    Purpose

    FRET acceptor: IQ6 domain of Moysin Va tagged with N-terminal mVenus

    Depositor

    Christian Wahl-Schott

    Article

    Feldmann et al STAR Protoc. 2023 Jul 28;4(3):102459. do

    Insert

    MYO5A IQ domain (Myo5a Mouse)
    

    Tags

    mVenus

    Expression

    Mammalian

    Available Since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti-CaMKIIa-hChR2(H134R)-mCherry-WPRE

    Plasmid

    #20943

    Purpose

    3rd gen lentiviral expression of humanized ChR2 with H134R mutation fused to mCherry driven by CaMKIIa promoter for optogenetic activation

    Depositor

    Karl Deisseroth

    Article

    Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.

    Insert

    channelrhodopsin-2
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    H134R

    Promoter

    CaMKIIa

    Available Since

    July 9, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSecTag-ATX

    Plasmid

    #17839

    Depositor

    Steven Rosen

    Article

    Kanda et al Nat Immunol. 2008 Apr . 9(4):415-23.

    Insert

    Autotaxin (ENPP2 Human)
    

    Expression

    Mammalian

    Available Since

    April 29, 2008

    Availability

    Academic Institutions and Nonprofits only

13. pCB24 pDEST crybb1:TagBFP

    Plasmid

    #195987

    Purpose

    Transgenesis backbone

    Depositor

    Christian Mosimann, Markus Affolter

    Article

    Kemmler et al Development. 2023 Mar 28:dev.201531. doi

    Type

    Empty backbone

    Use

    Other

    Available Since

    Feb. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET21b-Caspase-3

    Plasmid

    #90087

    Purpose

    Expresses wild type caspase 3 in bacterial cells

    Depositor

    Clay Clark

    Article

    Pop et al Biochemistry. 2001 Nov 27;40(47):14224-3

    Insert

    Caspase 3 (CASP3 Human)
    

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Wild type

    Available Since

    May 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET22b_PstS_3

    Plasmid

    #78200

    Purpose

    Expression of double-cysteine variant of mature phosphate binding protein, PstS, which also has a mutation to weaken phosphate binding, as scaffold for phosphate biosensor

    Depositor

    Martin Webb

    Article

    Solscheid et al Biochemistry. 2015 Aug 18;54(32):5054-62

    Insert

    pstS (A17C,I76G,A197C)
    

    Expression

    Bacterial

    Mutation

    A17C,I76G,A197C (please see depositor comment bel…

    Promoter

    T7

    Available Since

    July 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBK-CMV-hNHERF2

    Plasmid

    #47801

    Purpose

    Expresses human NHERF2 in mammalian cells

    Depositor

    Randy Hall

    Article

    Lau et al Biochemistry. 2001 Jul 24;40(29):8572-80

    Insert

    NHERF2 (NHERF2 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 3, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET21-pelB-LaTdT-39

    Plasmid

    #228980

    Purpose

    For bacterial expression of anti-tdTomato nanobody LaTdT-39, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

    Insert

    LaTdT-39
    

    Tags

    6xHIS

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCB59 pDEST crybb1:mKate2

    Plasmid

    #195986

    Purpose

    Transgenesis backbone

    Depositor

    Christian Mosimann, Markus Affolter

    Article

    Kemmler et al Development. 2023 Mar 28:dev.201531. doi

    Type

    Empty backbone

    Use

    Other

    Available Since

    Feb. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pME minprom KalTa4

    Plasmid

    #195964

    Purpose

    Gateway pME Gal4 variant

    Depositor

    Alexa Burger, Christian Mosimann

    Article

    Kemmler et al Development. 2023 Mar 28:dev.201531. doi

    Insert

    mouse beta-globin minimal promoter KalTA4 (Gal4)
    

    Use

    Other

    Available Since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. KP501: pMVP (L5-L4) eYFP-P2A

    Plasmid

    #121714

    Purpose

    pMVP L5-L4 entry plasmid, contains eYFP-P2A for 4-component MultiSite Gateway Pro assembly. Allows expression of N-term eYFP linked by P2A to gene of interest.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    eYFP-P2A (open)
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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