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  8. pDule-tfmF A65V S158A

    Plasmid
    #85484
    Purpose
    Machinery plasmid expressing permissive Mj tfmF synthetase and cognate amber suppressing tRNA
    Depositor
    Ryan Mehl
    Article
    Miyake-Stoner et al Biochemistry. 2010 Mar 2;49(8):1667-77.
    Insert
    tri-fluoromethyl-phenylalanine Mj synthetase A65V S158A
    Tags
    None
    Expression
    Bacterial
    Mutation
    Y32V F108W Q109M I159M with 65Val and 158Ala
    Promoter
    lpp (constitutive)
    Available Since
    Jan. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    85484-plasmid-map-sequence-id-169864

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  17. pENTR-R4R3-stop

    Plasmid
    #113745
    Purpose
    Contains three stop codons in every frame to ensure a premature stop codon when introduced into the genome via homology-directed repair. Gateway multisite entry clone.
    Depositor
    Magdalena Bezanilla
    Article
    Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d
    Insert
    stop cassette
    Use
    CRISPR; Gateway entry vector
    Mutation
    Added 3 stop codons to the original sequence; see…
    Available Since
    Sept. 24, 2018
    Availability
    Academic Institutions and Nonprofits only
    113745-plasmid-map-sequence-id-260896

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Showing: 2541 - 2560 of 9904 results