We narrowed to 16,262 results for: form

1. PA-DAD-wt

   Plasmid

   #53095

   Purpose

   Expresses wild type PA-DAD in mammalian cells, an optogenetic tool to activate endogenous diaphanous related formins in live cells using light.

   Depositor

   Klaus Hahn, Ronen Zaidel-Bar

   Article

   Rao et al Cytoskeleton (Hoboken). 2013 Jul;70(7):3

   Insert

   LOV(wt)-6aa-DAD
   

   Tags

   6x His and mVenus

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   L47F and C49S in Venus

   Available Since

   Aug. 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pIE-TGFB2-H6

   Plasmid

   #128500

   Purpose

   Expresses human pro-TGFβ2 (from L21 to S414) in insect cells. With C-t H6x tag.

   Depositor

   F Xavier Gomis-Ruth

   Article

   Marino-Puertas et al Sci Rep. 2019 Jun 24;9(1):9186. doi: 10.

   Insert

   TGFB2 (TGFB2 Human)
   

   Tags

   His tag (6x)

   Expression

   Insect

   Promoter

   IE1

   Available Since

   Aug. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pS6-TGFB2-H8

   Plasmid

   #128502

   Purpose

   Expresses human pro-TGFβ2 (from L21 to S414) in mammalian cells. With N-t H8x tag.

   Depositor

   F Xavier Gomis-Ruth

   Article

   Marino-Puertas et al Sci Rep. 2019 Jun 24;9(1):9186. doi: 10.

   Insert

   TGFB2 (L21-S414) (TGFB2 Human)
   

   Tags

   His tag (6x)

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. attB-mCh-Dnmt3b1-Poly(A)-PuroR

   Plasmid

   #65556

   Purpose

   Plasmid for Bxb1-mediated recombination of the mCh-Dnmt3b1 cDNA into a MIN-tagged locus using Puromycin selection

   Depositor

   Sebastian Bultmann

   Article

   Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

   Insert

   Dnmt3b (Dnmt3b Mouse)
   

   Use

   Mouse Targeting; Bxb1

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   isoform 1

   Available Since

   Aug. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. attB-mCh-Dnmt3b6-Poly(A)-NeoR

   Plasmid

   #65554

   Purpose

   Plasmid for Bxb1-mediated recombination of the mCh-Dnmt3b6 cDNA into a MIN-tagged locus using Neomycin selection

   Depositor

   Sebastian Bultmann

   Article

   Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

   Insert

   Dnmt3b (Dnmt3b Mouse)
   

   Use

   Mouse Targeting; Bxb1

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   isoform 6

   Available Since

   Aug. 31, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pS6-TGFB2-STREP

   Plasmid

   #128503

   Purpose

   Expresses human pro-TGFβ2 (from L21 to S414) in mammalian cells. With N-t STREP(2x) tag.

   Depositor

   F Xavier Gomis-Ruth

   Article

   Marino-Puertas et al Sci Rep. 2019 Jun 24;9(1):9186. doi: 10.

   Insert

   TGFB2 (TGFB2 Human)
   

   Tags

   STREP (2X) tag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. attB-mCh-Dnmt3b1-Poly(A)-NeoR

   Plasmid

   #65553

   Purpose

   Plasmid for Bxb1-mediated recombination of the mCh-Dnmt3b1 cDNA into a MIN-tagged locus using Neomycin selection

   Depositor

   Sebastian Bultmann

   Article

   Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

   Insert

   Dnmt3b (Dnmt3b Mouse)
   

   Use

   Mouse Targeting; Bxb1

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   isoform 1

   Available Since

   Aug. 31, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. attB-GFP-Dnmt3b1-Poly(A)-NeoR

   Plasmid

   #65551

   Purpose

   Plasmid for Bxb1-mediated recombination of the GFP-Dnmt3b1 cDNA into a MIN-tagged locus using Neomycin selection

   Depositor

   Sebastian Bultmann

   Article

   Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

   Insert

   Dnmt3b (Dnmt3b Mouse)
   

   Use

   Mouse Targeting; Bxb1

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   isoform 1

   Available Since

   Aug. 31, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGFP-mTet1s_K852R pc3914

   Plasmid

   #201704

   Purpose

   Expresses of GFP-tagged mTet1s_K852R in mammalian cells

   Depositor

   Cristina Cardoso

   Article

   Arroyo et al Nat Commun. 2022 Sep 2;13(1):5173. doi:

   Insert

   Tet1s-K852R (Tet1 Mouse)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Lysine 852 mutated to arginine

   Promoter

   CAG

   Available Since

   July 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGFP-mTet1s_K852E pc3915

    Plasmid

    #201705

    Purpose

    Expresses of GFP-tagged mTet1s_K852E in mammalian cells

    Depositor

    Cristina Cardoso

    Article

    Arroyo et al Nat Commun. 2022 Sep 2;13(1):5173. doi:

    Insert

    Tet1s-K852E (Tet1 Mouse)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Lysine 852 mutated to glutamate

    Promoter

    CAG

    Available Since

    July 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. CBSH3+ 3m shRNA-1

    Plasmid

    #160067

    Purpose

    3-point mutation negative control for CBSH3+ shRNA-1 targeting collybistin isoforms with SH3+ domain (plasmid 160066)

    Depositor

    Angel de Blas

    Article

    George et al J Neurochem. 2021 May;157(4):1032-1051.

    Insert

    ARHGEF9 (Arhgef9 Rat)
    

    Use

    RNAi

    Mutation

    GGcTGaTTaCAACAAGGATTG (mutations shown in lower c…

    Promoter

    mouse U6 promoter

    Available Since

    Oct. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. attB-GFP-Dnmt3b6-Poly(A)-NeoR

    Plasmid

    #65552

    Purpose

    Plasmid for Bxb1-mediated recombination of the GFP-Dnmt3b6 cDNA into a MIN-tagged locus using Neomycin selection

    Depositor

    Sebastian Bultmann

    Article

    Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

    Insert

    Dnmt3b (Dnmt3b Mouse)
    

    Use

    Mouse Targeting; Bxb1

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    isoform 6

    Available Since

    Aug. 31, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA5 CMV 3xFG eIF4G1

    Plasmid

    #158775

    Purpose

    pcDNA5 transfection plasmid for the exogenous expression of N-terminal 3xFlag-tagged eIF4G1 isoform lacking the microexon.

    Depositor

    Benjamin Blencowe

    Article

    Gonatopoulos-Pournatzis et al Mol Cell. 2020 Mar 19;77(6):1176-1192.e1

    Insert

    eIF4G1
    

    Tags

    3xFlag

    Expression

    Mammalian

    Available Since

    Aug. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1-Flag-PGC-1alpha1

    Plasmid

    #45501

    Depositor

    Bruce Spiegelman, Jorge Ruas

    Article

    Ruas et al Cell. 2012 Dec 7;151(6):1319-31. doi: 10

    Insert

    PGC-1alpha Isoform1 (Ppargc1a Mouse)
    

    Tags

    6xHis and Flag

    Expression

    Mammalian

    Mutation

    Q339K relative to NM_008904

    Available Since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pnCS_SEPT7_SEPT9_i1-TEV-Strep

    Plasmid

    #174500

    Purpose

    bacterial co-expression of human SEPT7 and of human SEPT9_i1

    Depositor

    Manos Mavrakis

    Article

    Iv et al J Cell Sci. 2021 Jul 8. pii: 270842. doi

    Inserts

    SEPTIN7 (SEPTIN7 Human)
    SEPTIN9 (SEPTIN9 Human)
    

    Tags

    TEV-Strep

    Expression

    Bacterial

    Available Since

    Sept. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA5 CMV 3xFG eIF4G1 +MIC

    Plasmid

    #158776

    Purpose

    pcDNA5 transfection plasmid for the exogenous expression of N-terminal 3xFlag-tagged eIF4G1 isoform including the microexon.

    Depositor

    Benjamin Blencowe

    Article

    Gonatopoulos-Pournatzis et al Mol Cell. 2020 Mar 19;77(6):1176-1192.e1

    Insert

    eIF4G1 (with microexon)
    

    Tags

    3xFlag

    Expression

    Mammalian

    Available Since

    Aug. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pnCS_SEPT7_SEPT9_i5-TEV-Strep

    Plasmid

    #174502

    Purpose

    bacterial co-expression of human SEPT7 and of human SEPT9_i5

    Depositor

    Manos Mavrakis

    Article

    Iv et al J Cell Sci. 2021 Jul 8. pii: 270842. doi

    Inserts

    SEPTIN7 (SEPTIN7 Human)
    SEPTIN9 (SEPTIN9 Human)
    

    Tags

    TEV-Strep

    Expression

    Bacterial

    Available Since

    Sept. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. Lenti-EFS-NTID194-GSQ-PMLivaWT-P2A-BLAST

    Plasmid

    #208038

    Purpose

    Enables constitutive expression of N-terminal Split-TurboID-fused PML (isoform IVa; WT); to perform SUMO-ID; selection with blasticidin

    Depositor

    James Sutherland, Rosa Barrio

    Article

    Barroso-Gomila et al Nat Commun. 2021 Nov 18;12(1):6671. doi:

    Insert

    PML (isoform IVa) (PML Human)
    

    Use

    Lentiviral

    Tags

    FLAG, N-terminal Split-TurboID

    Promoter

    EFS

    Available Since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pnCS_SEPT7_SEPT9_i3-TEV-Strep

    Plasmid

    #174501

    Purpose

    bacterial co-expression of human SEPT7 and of human SEPT9_i3

    Depositor

    Manos Mavrakis

    Article

    Iv et al J Cell Sci. 2021 Jul 8. pii: 270842. doi

    Inserts

    SEPTIN7 (SEPTIN7 Human)
    SEPTIN9 (SEPTIN9 Human)
    

    Tags

    TEV-Strep

    Expression

    Bacterial

    Available Since

    Sept. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. AAV phSyn1(S)-FlpE-bGHpA

    Plasmid

    #50364

    Purpose

    Can be used to generate AAV virus that will express FlpE recombinse gene in neurons from the synapsin promoter

    Depositor

    Hongkui Zeng

    Article

    Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

    Insert

    FlpE recombinase gene
    

    Use

    AAV

    Promoter

    hSyn1

    Available Since

    Jan. 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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