We narrowed to 3,491 results for: ttl

1. pEC2861 (pSpy1C-PgyrA_ffluc)

   Plasmid

   #218511

   Purpose

   replicative E. coli-S. pyogenes shuttle plasmid, constitutive expression of firefly luciferase reporter

   Depositor

   Emmanuelle Charpentier

   Article

   Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

   Insert

   ffluc
   

   Use

   Luciferase

   Mutation

   deletion of Plac_mrfp cassette

   Promoter

   PgyrA

   Available Since

   Sept. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. p201N Cas9

   Plasmid

   #59175

   Purpose

   Cas9 driven by double 35S, nptII for plant selection, I-PpoI site to accept gRNA from pUC gRNA Shuttle

   Depositor

   Wayne Parrott

   Article

   Jacobs et al BMC Biotechnol. 2015 Mar 12;15:16. doi:

   Inserts

   Cas9
   nptII
   

   Use

   CRISPR

   Promoter

   2x35S and StUbi-3P

   Available Since

   Sept. 26, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJK02

   Plasmid

   #105133

   Purpose

   Clostridium difficile CRISPR-cas9 mutagenesis plasmid traJ oriT

   Depositor

   Joseph Sorg

   Article

   McAllister et al Sci Rep. 2017 Nov 7;7(1):14672. doi: 10.

   Inserts

   Upstream & Downstream pyrE deletion region
   tetR
   Cas9
   gRNA
   

   Use

   E. coli - c. difficile shuttle vector

   Available Since

   Jan. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLX-TRV2

   Plasmid

   #180516

   Purpose

   Agrobacterium tumefaciens-based expression of TRV2 including a PEBV heterologous promoter and apolylinker

   Depositor

   Jose-Antonio Daros

   Article

   Aragones et al Biotechnol J. 2022 Mar 24:e2100504. doi:

   Insert

   Tobacco rattle virus RNA2 with a PEBV heterologous promoter and a polylinker
   

   Expression

   Plant

   Promoter

   35S CaMV

   Available Since

   April 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFW1000

   Plasmid

   #224210

   Purpose

   E. coli-Bacteroides shuttle plasmid; pB8-51 (Bacteroides replication origin), R6K (E. coli replication origin)

   Depositor

   Ophelia Venturelli

   Article

   Feng et al Cell Host Microbe. 2022 Feb 9;30(2):200-

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   Sept. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAD-sfGFP(Sp)-Pman

   Plasmid

   #133833

   Purpose

   Fluorescent protein sfGFP(Sp); under control of mannose inducible promoter.

   Depositor

   Oscar Kuipers

   Article

   Yi et al Environ Microbiol Rep. 2018 Feb;10(1):57

   Insert

   sfGFP(Sp)
   

   Use

   E.coli/bacillus spp. shuttle vector

   Expression

   Bacterial

   Promoter

   Pman

   Available Since

   Nov. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. p201H Cas9

   Plasmid

   #59176

   Purpose

   Cas9 driven by double 35S, hygromycin resistance for plant selection, I-PpoI site to accept gRNA from pUC gRNA Shuttle

   Depositor

   Wayne Parrott

   Article

   Jacobs et al BMC Biotechnol. 2015 Mar 12;15:16. doi:

   Inserts

   Cas9
   hph
   

   Use

   CRISPR

   Promoter

   2x35S and StUbi-3P

   Available Since

   Aug. 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEC3105 (pSpy1C-PxylS2_ffluc)

   Plasmid

   #218512

   Purpose

   replicative E. coli-S. pyogenes shuttle plasmid, constitutive expression of firefly luciferase reporter

   Depositor

   Emmanuelle Charpentier

   Article

   Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

   Insert

   ffluc
   

   Use

   Luciferase

   Mutation

   deletion of Plac_mrfp cassette

   Promoter

   PxylS2

   Available Since

   Sept. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDGE332

   Plasmid

   #153241

   Purpose

   M1 shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

   Depositor

   Johannes Stuttmann

   Article

   Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDGE333

    Plasmid

    #153242

    Purpose

    M2 shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

    Depositor

    Johannes Stuttmann

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pOCC151

    Plasmid

    #118899

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal GST, cleavable with 3C

    Depositor

    PEPC MPI CBG, Marc Bickle

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-GST-3C-NotI-ccdB-AscI-stop-HindIII cassette
    

    Tags

    GST tag, cleavable with 3C protease

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDGE335

    Plasmid

    #153244

    Purpose

    M3 shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

    Depositor

    Johannes Stuttmann

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAP114

    Plasmid

    #120799

    Purpose

    E. coli-C. difficile shuttle vector for xylose-inducible expression in C. difficile; Pxyl driving expression of red fluorescent protein (mCherryOpt)

    Depositor

    David S. Weiss, Craig Ellermeier

    Article

    Muh et al J Bacteriol. 2019 Feb 11. pii: JB.00711-

    Insert

    Pxyl::mCherryOpt
    

    Expression

    Bacterial

    Mutation

    1.4 kb xylR-Pxyl DNA fragment from C. difficile R…

    Promoter

    Pxyl

    Available Since

    Feb. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. hSERT-N1-pEYFP

    Plasmid

    #70105

    Purpose

    mammalian expression of hSERT with C-terminal YFP fusion

    Depositor

    Harald Sitte

    Article

    Just et al J Biol Chem. 2004 Feb 20;279(8):6650-7.

    Insert

    SERT (SLC6A4 Human)
    

    Tags

    EYFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDGE496

    Plasmid

    #153248

    Purpose

    M6 shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

    Depositor

    Johannes Stuttmann

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    Sept. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDGE495

    Plasmid

    #153247

    Purpose

    M5 shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

    Depositor

    Johannes Stuttmann

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDGE336

    Plasmid

    #153245

    Purpose

    M4 shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

    Depositor

    Johannes Stuttmann

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pDGE341

    Plasmid

    #153250

    Purpose

    M7 shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

    Depositor

    Johannes Stuttmann

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBT346_pLambda-attB-pCA-GFP-pA

    Plasmid

    #52551

    Purpose

    for producing PhiC31 attB-containing minicircle in vitro

    Depositor

    Liqun Luo

    Article

    Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

    Inserts

    Lambda integrase attL1
    Lambda integrase attR1
    PhiC31o attB (full length)
    GFP
    

    Expression

    Mammalian

    Available Since

    Aug. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pXD70LacZ1-promoterless-LacZ

    Plasmid

    #191617

    Purpose

    E. coli - Eggerthella lenta shuttle plasmid (KanR), promoterless-lacZ

    Depositor

    Emily Balskus

    Article

    Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

    Insert

    lacZ
    

    Expression

    Bacterial

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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