Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more


(Plasmid #113880)


Item Catalog # Description Quantity Price (USD)
Plasmid 113880 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
    pDONR P2R-P3
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 2640
  • Total vector size (bp) 4439
  • Vector type
    Zebrafish Danio expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Growth instructions
    10 ug/ml kanamycin final concentration of LB media
  • Copy number
    High Copy


  • Gene/Insert name
  • Alt name
    hatching gland promoter mCerulean
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
  • Entrez Gene
    he1.1 (a.k.a. cb284, he1, he1a, wu:cegs2099, zgc:111847)
  • Tag / Fusion Protein
    • ECFP (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Cloned from the construct provided by Jeff Mumm's Lab, Tol2 5xUAS-mCFP-p2A-NTRmut;HE:mCerulean

Reference for the he1a promoter was first described in:
Xie et al., "Silencer-delimited transgenesis: NRSE/RE1 sequences promote neural-specific transgene expression
in a NRSF/REST-dependent manner​."
BMC Biology 2012 10:93

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p3E_he1a:ECFP was a gift from Marnie Halpern (Addgene plasmid # 113880 ; ; RRID:Addgene_113880)