|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||113880||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepDONR P2R-P3
- Backbone size w/o insert (bp) 2640
- Total vector size (bp) 4439
Vector typeZebrafish Danio expression
Growth in Bacteria
Growth instructions10 ug/ml kanamycin final concentration of LB media
Copy numberHigh Copy
Alt namehatching gland promoter mCerulean
SpeciesD. rerio (zebrafish)
Insert Size (bp)1799
Entrez Genehe1.1 (a.k.a. cb284, he1, he1a, wu:cegs2099, zgc:111847)
/ Fusion Protein
- ECFP (C terminal on insert)
- Cloning method Gateway Cloning
- 5′ sequencing primer M13F
- 3′ sequencing primer M13R (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Cloned from the construct provided by Jeff Mumm's Lab, Tol2 5xUAS-mCFP-p2A-NTRmut;HE:mCerulean
Reference for the he1a promoter was first described in:
Xie et al., "Silencer-delimited transgenesis: NRSE/RE1 sequences promote neural-specific transgene expression
in a NRSF/REST-dependent manner."
BMC Biology 2012 10:93
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:p3E_he1a:ECFP was a gift from Marnie Halpern (Addgene plasmid # 113880 ; http://n2t.net/addgene:113880 ; RRID:Addgene_113880)