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p3E_he1a:eYFP
(Plasmid #113879)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 113879 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pDONR P2R-P3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 2618
  • Total vector size (bp) 4292
  • Vector type
    Zebrafish Danio expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    10 ug/ml kanamycin final concentration of LB media
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    he1a
  • Alt name
    hatching gland promoter eYFP
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
    1674
  • Entrez Gene
    he1.1 (a.k.a. cb284, he1, he1a, wu:cegs2099, zgc:111847)
  • Tag / Fusion Protein
    • eYFP (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Cloned from the construct provided by Jeff Mumm's Lab, pTol2 5xUAS:YFP-p2A-NTRmutgi:HE:eYFP

Reference for the he1a promoter was first described in:
Xie et al., "Silencer-delimited transgenesis: NRSE/RE1 sequences promote neural-specific transgene expression
in a NRSF/REST-dependent manner​."
BMC Biology 2012 10:93
https://doi.org/10.1186/1741-7007-10-93

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p3E_he1a:eYFP was a gift from Marnie Halpern (Addgene plasmid # 113879 ; http://n2t.net/addgene:113879 ; RRID:Addgene_113879)