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(Plasmid #72640)


Item Catalog # Description Quantity Price (USD)
Plasmid 72640 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
    pDONR 221
  • Backbone manufacturer
  • Total vector size (bp) 11596

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    elavl3 enhancer
  • Alt name
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
  • Entrez Gene
    elavl3 (a.k.a. HuC, elrc, id:ibd1248, wu:fb77b03, zHuC)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (unknown if destroyed)
  • 3′ cloning site SalI (unknown if destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T3
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The p5E-elavl3 enhancer clone was generated by inserting the XhoI/SalI fragment from pCS2-HuC:Kaede (Sato et al., 2006) (Hitoshi Okamoto lab) into the XhoI site of p5E-MCS.
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Fujimoto E, Gaynes B, Brimley CJ, Chien CB, Bonkowsky JL. Gal80 intersectional regulation of cell-type specific expression in vertebrates. Dev Dyn. 2011 Oct;240(10):2324-34. doi: 10.1002/dvdy.22734. Epub 2011 Sep 8. PubMed PMID:21905164; PubMed Central PMCID: PMC3178006.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p5E-elavl3 was a gift from Joshua Bonkowsky (Addgene plasmid # 72640 ; ; RRID:Addgene_72640)