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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 4,802 results for: pcr

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Showing: 2581 - 2600 of 4802 results

  1. LTB4R-Tango

    Plasmid
    #66423
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    LTB4R (LTB4R Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only

  5. mutpRL-TK 1x perfect to bulged siRNA

    Plasmid
    #40765
    Purpose
    To evaluate the efficacy of silencing and the extent of cooperativity directed by a small silencing RNA bound at one or multiple sites on an mRNA
    Depositor
    Phillip Zamore
    Article
    Broderick et al RNA. 2011 Oct;17(10):1858-69. Epub 2011
    Insert
    CXCR4 small RNA perfect to bulged target site
    Use
    Luciferase
    Tags
    luciferase
    Mutation
    mutated from TAG to CTC at nt 387–389 of the Reni…
    Promoter
    TK
    Available Since
    Dec. 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  6. mutpRL-TK 1x perfect to seed siRNA

    Plasmid
    #40766
    Purpose
    To evaluate the efficacy of silencing and the extent of cooperativity directed by a small silencing RNA bound at one or multiple sites on an mRNA
    Depositor
    Phillip Zamore
    Article
    Broderick et al RNA. 2011 Oct;17(10):1858-69. Epub 2011
    Insert
    CXCR4 small RNA perfect to seed target site
    Use
    Luciferase
    Tags
    luciferase
    Mutation
    mutated from TAG to CTC at nt 387–389 of the Reni…
    Promoter
    TK
    Available Since
    Sept. 26, 2013
    Availability
    Academic Institutions and Nonprofits only

  11. mutpRL-TK 1x perfect to seed plus 13-16 siRNA

    Plasmid
    #40767
    Purpose
    To evaluate the efficacy of silencing and the extent of cooperativity directed by a small silencing RNA bound at one or multiple sites on an mRNA
    Depositor
    Phillip Zamore
    Article
    Broderick et al RNA. 2011 Oct;17(10):1858-69. Epub 2011
    Insert
    CXCR4 small RNA perfect to seed plus 13-16 target site
    Use
    Luciferase
    Tags
    luciferase
    Mutation
    mutated from TAG to CTC at nt 387–389 of the Reni…
    Promoter
    TK
    Available Since
    Sept. 26, 2013
    Availability
    Academic Institutions and Nonprofits only

  12. TRE-RXRa-Myc; pGK-H2B-RFP

    Plasmid
    #242887
    Purpose
    For lentiviral overexpression of Myc-tagged human RXRa coupled to H2B-RFP (in the opposite direction)
    Depositor
    Elaine Fuchs
    Article
    Stewart et al Nature. 2024 Sep;633(8029):407-416. doi:
    Insert
    Rxra (RXRA Human)
    Use
    Lentiviral
    Tags
    H2B-mRFP
    Expression
    Mammalian
    Mutation
    Human RXRa cDNA was PCR-amplified from pSV-Sport-…
    Promoter
    Tetracycline response element upstream of a minim…
    Available Since
    Aug. 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    242887-plasmid-map-sequence-id-484988

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  14. pCEP4-HA-Flag-natT1R2 FS1

    Plasmid
    #113955
    Purpose
    mammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin; expression-enhanced variant
    Depositor
    Erik Procko
    Article
    Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061
    Insert
    T1R2 (TAS1R2 Human)
    Tags
    FLAG and Signal/leader sequence from influenza he…
    Expression
    Mammalian
    Mutation
    delete N-terminal 21 residues, one base pair dele…
    Promoter
    cmv
    Available Since
    Feb. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    113955-plasmid-map-sequence-id-215169

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  15. GGE105

    Plasmid
    #120734
    Purpose
    Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Auxotrophy marker for selection after transformation in Y. lipolytica (Marker_Leu2)
    Depositor
    Tristan Rossignol
    Article
    Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.
    Insert
    Marker_Leu2
    Use
    Cloning/assembly plasmid
    Available Since
    Oct. 10, 2019
    Availability
    Academic Institutions and Nonprofits only
    120734-plasmid-map-sequence-id-259146

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  17. GGE151

    Plasmid
    #120751
    Purpose
    Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Promoter for gene expression in position 2 (P2_4UASxpr-TEF)
    Depositor
    Tristan Rossignol
    Article
    Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.
    Insert
    P2_4UASxpr-TEF
    Use
    Cloning/assembly plasmid
    Available Since
    Nov. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    120751-plasmid-map-sequence-id-262885

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  18. GGE142

    Plasmid
    #120735
    Purpose
    Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Metabolic marker for selection after transformation in Y. lipolytica (Marker_Suc2)
    Depositor
    Tristan Rossignol
    Article
    Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.
    Insert
    Marker_Suc2
    Use
    Cloning/assembly plasmid
    Available Since
    Oct. 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    120735-plasmid-map-sequence-id-259147

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Showing: 2581 - 2600 of 4802 results