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pLJM1-SLC35B2-FLAG

Plasmid

#154863

Purpose

Transient or Lentiviral expression of sgRNA (Addgene#154860) resistant SLC35B2

Depositor

Yihong Ye

Article

Zhang et al Proc Natl Acad Sci U S A. 2020 May 19;11

Insert

SLC35B2 (SLC35B2 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

ctcctggtgcagtacttc => ttattagtccaatatttt

Promoter

CMV

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA6-E2F4CA-Myc

Plasmid

#80428

Purpose

Mammalian expression of constitutively active chick E2F4

Depositor

José María Frade

Article

Morillo et al Mol Cell Biol. 2012 Jul;32(14):2722-37.

Insert

E2F transcription factor 4 (E2F4 Chicken)

Tags

Myc

Expression

Mammalian

Mutation

T261E, T263E

Promoter

CMV

Available Since

July 27, 2016

Availability

Academic Institutions and Nonprofits only

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TAPBPL pEGFP-C3 (1765)

Plasmid

#62055

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Korfali et al PLoS One. 2011 Apr 14;6(4):e18762. doi:

Insert

TAPBPL (TAPBPL Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 22, 2015

Availability

Academic Institutions and Nonprofits only
pNUT N6His K206E hTFNG

Plasmid

#70130

Purpose

Expresses N-His tagged nonglycosylated human serum transferrin unable to release iron from the N-lobe

Depositor

Ross MacGillivray, Anne Mason

Article

He et al Biochemistry. 1999 Jul 27;38(30):9704-11

Insert

mutated human serum transferrin (TF Human)

Tags

N-terminal signal peptide, 4 aa link, 6 His, Fact…

Expression

Mammalian

Mutation

changed Lys206 to a glutamic acid, Asn413Asp, Asn…

Promoter

SV40

Available Since

Nov. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pHIV-IL13-TetCoil

Plasmid

#240244

Purpose

Lentiviral transfer plasmid encoding IL13 on tetrameric coiled coil

Depositor

Michael Birnbaum

Article

Dobson et al Nat Methods. 2022 Apr;19(4):449-460. doi

Insert

IL-13 (IL13 Human)

Use

Lentiviral

Tags

EGFP and HA

Expression

Mammalian

Promoter

EF1a

Available Since

July 18, 2025

Availability

Academic Institutions and Nonprofits only

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CMV-NF186(K519TAG)-HA

Plasmid

#239775

Purpose

Expresses rat NF186 with a TAG codon at position 519 and a C-terminal HA tag in mammalian cells. Can be used for click labeling.

Depositor

Ivana Nikić-Spiegel

Article

Stajkovic et al J Cell Sci. 2023 Jun 15;136(12):jcs26060

Insert

Nfasc (Nfasc Rat)

Tags

HA-tag

Expression

Mammalian

Mutation

K519TAG

Promoter

CMV

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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CMV-NF186(K534TAG)-HA

Plasmid

#239776

Purpose

Expresses rat NF186 with a TAG codon at position 534 and a C-terminal HA tag in mammalian cells. Can be used for click labeling.

Depositor

Ivana Nikić-Spiegel

Article

Stajkovic et al J Cell Sci. 2023 Jun 15;136(12):jcs26060

Insert

Nfasc (Nfasc Rat)

Tags

HA-tag

Expression

Mammalian

Mutation

K534TAG

Promoter

CMV

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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CMV-NF186(K571TAG)-HA

Plasmid

#239777

Purpose

Expresses rat NF186 with a TAG codon at position 571 and a C-terminal HA tag in mammalian cells. Can be used for click labeling.

Depositor

Ivana Nikić-Spiegel

Article

Stajkovic et al J Cell Sci. 2023 Jun 15;136(12):jcs26060

Insert

Nfasc (Nfasc Rat)

Tags

HA-tag

Expression

Mammalian

Mutation

K571TAG

Promoter

CMV

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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CMV-NF186(K604TAG)-HA

Plasmid

#239778

Purpose

Expresses rat NF186 with a TAG codon at position 604 and a C-terminal HA tag in mammalian cells. Can be used for click labeling.

Depositor

Ivana Nikić-Spiegel

Article

Stajkovic et al J Cell Sci. 2023 Jun 15;136(12):jcs26060

Insert

Nfasc (Nfasc Rat)

Tags

HA-tag

Expression

Mammalian

Mutation

K604TAG

Promoter

CMV

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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CMV-NF186(K680TAG)-HA

Plasmid

#239779

Purpose

Expresses rat NF186 with a TAG codon at position 680 and a C-terminal HA tag in mammalian cells. Can be used for click labeling.

Depositor

Ivana Nikić-Spiegel

Article

Stajkovic et al J Cell Sci. 2023 Jun 15;136(12):jcs26060

Insert

Nfasc (Nfasc Rat)

Tags

HA-tag

Expression

Mammalian

Mutation

K680TAG

Promoter

CMV

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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CMV-NF186(K809TAG)-HA

Plasmid

#239780

Purpose

Expresses rat NF186 with a TAG codon at position 809 and a C-terminal HA tag in mammalian cells. Can be used for click labeling.

Depositor

Ivana Nikić-Spiegel

Article

Stajkovic et al J Cell Sci. 2023 Jun 15;136(12):jcs26060

Insert

Nfasc (Nfasc Rat)

Tags

HA-tag

Expression

Mammalian

Mutation

K809TAG

Promoter

CMV

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pHIV-IL13-GAPGAS-PDGFR

Plasmid

#240246

Purpose

Lentiviral transfer plasmid encoding IL13 with GAPGAS linker and PDGFR TM

Depositor

Michael Birnbaum

Article

Dobson et al Nat Methods. 2022 Apr;19(4):449-460. doi

Insert

IL-13 (IL13 Human)

Use

Lentiviral

Tags

EGFP and HA

Expression

Mammalian

Promoter

EF1a

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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pJExpress401-KDAC8 D101N

Plasmid

#224348

Purpose

Expresses human KDAC8 (HDAC8) D101N in E. coli

Depositor

Terry Watt

Article

Toro et al Biochemistry. 2021 Aug 24;60(33):2524-25

Insert

KDAC8 (HDAC8 Human)

Tags

TEV-cleavable His6

Expression

Bacterial

Mutation

Aspartate 101 mutated to asparagine

Promoter

T5

Available Since

Feb. 19, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pME-zdgat2-CDS-FLAG

Plasmid

#232142

Purpose

Gateway middle entry vector containing zebrafish dgat2 with a C-terminal 3xFLAG tag

Depositor

Steven Farber

Article

Wilson et al J Biol Chem. 2024 Nov 6;300(12):107973.

Insert

dgat2 (dgat2 Zebrafish)

Use

Gateway cloning middle entry

Tags

3xFLAG

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pAcBac1-Pyl-TAA

Plasmid

#228783

Purpose

Pyl aaRS/tRNA system for mammalian TAA suppression

Depositor

Abhishek Chatterjee

Article

Osgood et al Angew Chem Int Ed Engl. 2023 May 2;62(19

Inserts

PylRS
PytR

Use

Baculoviral

Expression

Mammalian

Mutation

TAA suppressor

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pJExpress401-KDAC8 D101R

Plasmid

#224349

Purpose

Expresses human KDAC8 (HDAC8) D101R in E. coli

Depositor

Terry Watt

Article

Toro et al Biochemistry. 2021 Aug 24;60(33):2524-25

Insert

KDAC8 (HDAC8 Human)

Tags

TEV-cleavable His6

Expression

Bacterial

Mutation

Aspartate 101 mutated to arginine

Promoter

T5

Available Since

Oct. 28, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pJExpress401-KDAC8 D101A

Plasmid

#224347

Purpose

Expresses human KDAC8 (HDAC8) D101A in E. coli

Depositor

Terry Watt

Article

Toro et al Biochemistry. 2021 Aug 24;60(33):2524-25

Insert

KDAC8 (HDAC8 Human)

Tags

TEV-cleavable His6

Expression

Bacterial

Mutation

Aspartate 101 mutated to alanine

Promoter

T5

Available Since

Oct. 22, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pYPQ265E7

Plasmid

#213470

Purpose

Gateway entry clone (attL1 & attR5) TadA-CDd-32aa linker-zSpCas9-D10A-2xUGI for C-T base editing

Depositor

Yiping Qi

Article

Fan et al Nat Commun. 2024 Jun 14;15(1):5103. doi:

Insert

TadA-CDd-32aa linker-zSpCas9-D10A-2xUGI

Use

CRISPR; Gateway compatible tada-cdd-32aa linker-z…

Tags

No

Expression

Plant

Available Since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pYPQ265E8

Plasmid

#213471

Purpose

Gateway entry clone (attL1 & attR5) TadA-CDd_V106W-32aa linker-zSpCas9-D10A-2xUGI for C-T base editing

Depositor

Yiping Qi

Article

Fan et al Nat Commun. 2024 Jun 14;15(1):5103. doi:

Insert

TadA-CDd_V106W-32aa linker-zSpCas9-D10A-2xUGI

Use

CRISPR; Gateway compatible tada-cdd_v106w-32aa li…

Tags

No

Expression

Plant

Available Since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-DONSON-DEWS

Plasmid

#208799

Purpose

Xenopus laevis His6-Myc-DONSON (D374A, E377A, W381A, S437A)

Depositor

Aga Gambus

Article

Cvetkovic et al Mol Cell. 2023 Nov 16;83(22):4017-4031.e

Insert

DONSON (donson.L Frog)

Tags

His and Myc

Expression

Bacterial

Mutation

D374A; E377A; W381A; S437A

Promoter

T7

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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