We narrowed to 9,247 results for: CAG

1. pCAG-lox-rox-STOP-rox-tRFP-pA-lox-ZsGreen-pA

   Plasmid

   #175438

   Purpose

   dual plasmid labelling cells of different neuronal population with different fluorophores

   Depositor

   Sebastián Dupraz, Frank Bradke

   Article

   Alfadil et al J Vis Exp. 2021 Oct 14;(176). doi: 10.37

   Insert

   rox-STOP-rox-tRFP-pA
   

   Use

   Cre/Lox; Dre/rox

   Tags

   ZsGreen on backbone

   Expression

   Mammalian

   Mutation

   Promoter

   pCAG

   Available since

   Oct. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG-hdAsCas12a(D908A)-NLS(nucleoplasmin)-gs-3xHA-gs-VPR (RTW876)

   Plasmid

   #114078

   Purpose

   CAG promoter expression plasmid for human codon optimized AsCas12a-VPR(1.1)

   Depositor

   Benjamin Kleinstiver, Keith Joung

   Article

   Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

   Insert

   human codon optimized DNase-inactive (D908A) enAsCas12a fused to VPR activation domain
   

   Use

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. R26-CAG-loxP-FNF-bpA-3pA-loxP-tdTomato

   Plasmid

   #180152

   Purpose

   tdTomato cre reporter with Rosa26 recombineering homology arms

   Depositor

   Mario Capecchi

   Insert

   tdTomato
   

   Use

   Cre/Lox and Mouse Targeting

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   Feb. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCA26-pLenti.CAG.-NLS-dCas13b-NLS-VPR-T2A-HaloTag

   Plasmid

   #199457

   Purpose

   Expression of dCas13-VPR in mammalian cells

   Depositor

   Baohui Chen

   Article

   Liang et al Nat Commun. 2022 Nov 28;13(1):7329. doi:

   Insert

   dCas13
   

   Use

   Lentiviral

   Tags

   VPR-T2A-HaloTag

   Expression

   Mammalian

   Mutation

   Nuclease-dead H133A and H1058A

   Promoter

   CAG

   Available since

   May 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. PBRPyCAG-LoxP-ERAS-STOPcassette-Loxp-DsRed-IRES-PURO

   Plasmid

   #52419

   Purpose

   Constitutive mammalian expression of ERAS. Upon CRE mediated deletion of ERAS insert, dsRED expression markers is truned on due to concomittant removal of stop cassette.

   Depositor

   Jacob Hanna

   Article

   Gafni et al Nature. 2013 Dec 12;504(7479):282-6. doi

   Insert

   ERAS (ERAS Human)
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   CAGGS

   Available since

   Sept. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-CAG-Flex-mRuby2-GSG-P2A-GCaMP6s-WPRE-pA

   Plasmid

   #68717

   Purpose

   Cre-dependent bicistronic vector expressing mRuby2 and GCaMP6s from a single open reading frame.

   Depositor

   Mark Huebener, Tobias Bonhoeffer, Tobias Rose

   Article

   Rose et al Science. 2016 Jun 10;352(6291):1319-22.

   Has Service

   AAV1

   Insert

   mRuby2-P2A-GCaMP6s
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Cre-dependent expression from inverted open readi…

   Promoter

   CAG-FLEX

   Available since

   June 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-CAG-Flex-mRuby2-GSG-P2A-GCaMP6f-WPRE-pA

   Plasmid

   #68719

   Purpose

   Cre-dependent bicistronic vector expressing mRuby2 and GCaMP6f from a single open reading frame.

   Depositor

   Mark Huebener, Tobias Bonhoeffer, Tobias Rose

   Article

   Rose et al Science. 2016 Jun 10;352(6291):1319-22.

   Has Service

   AAV1

   Insert

   mRuby2-P2A-GCaMP6f
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   CAG-FLEX

   Available since

   June 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMB953:PB-CAG-PuroR-nGFP-FLEx-mCherry-KASH-WPRE

   Plasmid

   #168106

   Purpose

   PiggyBac transposon vector constitutively driving PuroR-P2A-eGFP and Cre conditional expression of KASH nuclear membrane-bound mCherry. Conditional insert flanked by MCS for cloning

   Depositor

   Erich Jarvis

   Article

   Biegler et al Sci Rep. 2022 Mar 14;12(1):4369. doi: 10

   Insert

   mCherry-KASH
   

   Use

   CRISPR and Cre/Lox

   Tags

   Expression

   Mutation

   Promoter

   CAG

   Available since

   May 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. H11-CAG-loxP-FNF-bpA-3pA-loxP-tdTomato

   Plasmid

   #180156

   Purpose

   tdTomato cre reporter with Hipp11 recombineering homology arms

   Depositor

   Mario Capecchi

   Insert

   tdTomato
   

   Use

   Cre/Lox and Mouse Targeting

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   April 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-CMV-BDLacZ-SAS620-3'Luciferase (split CAGGT)-SV40pA

    Plasmid

    #216321

    Purpose

    Split luciferase assay to test reconstitution via mRNA trans-splicing (REVeRT system).

    Depositor

    Elvir Becirovic

    Article

    Riedmayr et al Nat Commun. 2023 Oct 18;14(1):6578. doi:

    Insert

    Split Luciferase + splice acceptor site
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGL3-sgRANGAP.C1-CAG-Cas9-T2A-mCherry-P2A-Puro

    Plasmid

    #216249

    Purpose

    Express Cas9 with CAG promoter to improve the expression in hES cells and sgRANGAP.C1 to tag endogenous RANGAP1 C-terminus

    Depositor

    Elina Ikonen

    Article

    Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:

    Insert

    Cas9 and sgRANGAP.C1 (RANGAP1 Human)
    

    Use

    CRISPR; Endogenous tagging

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. R26-CAG-rox-FNF-bpA-3pA-rox-td-sfGFP

    Plasmid

    #180153

    Purpose

    td-sfGFP dre reporter with Rosa26 recombineering homology arms

    Depositor

    Mario Capecchi

    Insert

    td-sfGFP
    

    Use

    Mouse Targeting; Dre/rox

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAG-lox-inv[Talpha1-iCre-pA]-lox-mNeonGreen-hCentrin1

    Plasmid

    #196890

    Purpose

    Neuron-specific expression of human (h)Centrin fused to mNeonGreen

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    mNeonGreen-hCentrin1 (CETN1 Human)
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Quimeric CAG

    Available since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBW2614_pCAG-PV1-GAI-L2-28-396-FlpO-ABI-NLS-BGHpA

    Plasmid

    #108833

    Purpose

    Expresses aa28 to aa396 of Flp recombinase fused to GAI and ABI CIDs

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

    Insert

    Flp recombinase fragment aa28 to aa396
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    ABI,NLS and GAI

    Expression

    Mammalian

    Mutation

    Promoter

    pCAG

    Available since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. LZF1604V2: CAG-spine-jRGECO1a-P2A-QuasAr3-dark Citrine

    Plasmid

    #104122

    Purpose

    synOptopatch construct

    Depositor

    Adam Cohen

    Article

    Fan et al Nat Methods. 2018 Oct;15(10):823-831. do

    Insert

    spine-jRGECO1a-P2A-QuasAr-dark Citrine
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. CAG-loxP-3xpA-loxP-tdTomato+RbG and HsbG syntrons

    Plasmid

    #172479

    Purpose

    tdTomato with synthetic introns derived from the rabbit beta-globin gene and the human beta-globin gene

    Depositor

    Mario Capecchi

    Insert

    tdTomato
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAG-hAsCas12a-RR(S542R/K607R)-NLS(nucleoplasmin)-3xHA (AAS1081)

    Plasmid

    #114094

    Purpose

    CAG promoter expression plasmid for human codon optimized AsCas12a-RR(S542R/K607R) nuclease with C-terminal NLS and HA tag

    Depositor

    Benjamin Kleinstiver, Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    human codon optimized AsCas12a-RR (S542R/K607R)
    

    Use

    Tags

    NLS(nucleoplasmin) and 3xHA

    Expression

    Mammalian

    Mutation

    S542R and K607R

    Promoter

    Available since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. AAVS1-Puro CAG-CLTX CD32a ITAM CD3z CAR

    Plasmid

    #171965

    Purpose

    Donor plasmid for CLTX CD32a-ITAM CD3z CAR expression in human cells

    Depositor

    Xiaoping Bao

    Article

    Chang et al Nat Commun. 2023 Apr 20;14(1):2266. doi:

    Insert

    Synthetic CLTX CD32a ITAM CD3z CAR
    

    Use

    CRISPR, Synthetic Biology, and TALEN

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. Cav2.1 HA EI,II,III,IVA (E320A, E670A, E1411A, E1707A) pCAGGS

    Plasmid

    #206115

    Purpose

    expression of rat Cav2.1 with an exofacial double HA tag in domain II and mutations in the P loop of Domains I, II, III and IV which abolish trafficking to the cell surface

    Depositor

    Annette Dolphin

    Article

    Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi

    Insert

    cacna1b (Cacna1a Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E320A, E670A, E1411A, E1707A

    Promoter

    CMV/B-actin

    Available since

    Nov. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCDH-Lenti-CAGGS-hPRDM6-2xGly-V5-EF1-Luc2-Blast

    Plasmid

    #213826

    Purpose

    Expresses human PRDM6 with a C-terminal V5 epitope tag.

    Depositor

    Bjoern Schwer

    Article

    Schmidt et al bioRxiv. 2023 Aug 31:2023.08.29.555389.

    Insert

    PRDM6 (PRDM6 Human)
    

    Use

    Lentiviral and Luciferase

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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