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We narrowed to 5,282 results for: golden gate

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Showing: 2661 - 2680 of 5282 results

  12. EBOV 4cis

    Plasmid
    #249332
    Purpose
    Integration plasmid encoding EBOV essential replication factors (NP, VP35, VP30, and L protein)
    Depositor
    Amy Kistler
    Insert
    EBOV NP-P2A-VP35-P2A-VP30-P2A-L protein
    Use
    Piggybac donor vector
    Expression
    Bacterial and Mammalian
    Mutation
    the EBOV NP, VP35, and VP30 ORF sequences within …
    Promoter
    CAG
    Available since
    Jan. 22, 2026
    Availability
    Academic Institutions and Nonprofits only
    249332-plasmid-map-sequence-id-497977

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  13. pTL50_cHS4-CAG-s36GFPmNG-t2A-PuroR-p2A-BFP-polyAcHS4

    Plasmid
    #223541
    Purpose
    PUFFFIN cassette with s36GFP-mNeonGreen and tagBFP, strong constitutive promoter CAG, insulated by cHS4 for random integration, puromycin resistance for selection in mammalian cells
    Depositor
    Sally Lowell
    Publication
    Lebek et al EMBO J. 2024 Jul 12. doi: 10.1038/s44318
    Insert
    s36GFP-mNeonGreen, tagBFP, PAC
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    Jan. 21, 2026
    Availability
    Academic Institutions and Nonprofits only
    223541-plasmid-map-sequence-id-442162

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  19. pEvolvR-nCas9(D10A)-PolI5MΔ

    Plasmid
    #249069
    Purpose
    Expresses nucleus-localized nCas9 (D10A) fused to PolI5MΔ and mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs.
    Depositor
    John Dueber, David Schaffer
    Publication
    Hurtado et al Nat Commun. 2025 Apr 19;16(1):3723. doi:
    Insert
    nCas9-PolI5MΔ
    Use
    CRISPR
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    deletion of PolI5M N-terminal flap endonuclease d…
    Promoter
    CMV
    Available since
    Jan. 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    249069-plasmid-map-sequence-id-496321

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  20. pEvolvR-mCherry-Slug-nCas9(D10A)-PolI5MΔ

    Plasmid
    #249078
    Purpose
    Expresses nucleus-localized Slug-nCas9 (D10A) fused to PolI5MΔ and mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs.
    Depositor
    John Dueber, David Schaffer
    Publication
    Hurtado et al Nat Commun. 2025 Apr 19;16(1):3723. doi:
    Insert
    Slug-nCas9-PolI5MΔ
    Use
    CRISPR
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    deletion of PolI5M N-terminal flap endonuclease d…
    Promoter
    CMV
    Available since
    Jan. 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    249078-plasmid-map-sequence-id-496311

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Showing: 2661 - 2680 of 5282 results