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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 5,185 results for: golden gate

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Showing: 2681 - 2700 of 5185 results

  1. pMV_AA104

    Plasmid
    #245326
    Purpose
    Cas9 CRISPRa positive control guide; targets CD4
    Depositor
    John Doench, David Root
    Insert
    sgRNA CD4 (CD4 Synthetic)
    Use
    Lentiviral
    Mutation
    None
    Available Since
    Oct. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    245326-plasmid-map-sequence-id-488034

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  2. pMV_AA102

    Plasmid
    #245324
    Purpose
    Cas9 CRISPRa positive control guide; targets CD47
    Depositor
    John Doench, David Root
    Insert
    sgRNA CD47 (CD47 Synthetic)
    Use
    Lentiviral
    Mutation
    None
    Available Since
    Oct. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    245324-plasmid-map-sequence-id-488026

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  3. pRDB_248

    Plasmid
    #245335
    Purpose
    Cas12a CRISPRko positive control guide; targets CD63
    Depositor
    John Doench, David Root
    Insert
    sgRNA CD63 (CD63 Synthetic)
    Use
    Lentiviral
    Mutation
    None
    Available Since
    Oct. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    245335-plasmid-map-sequence-id-488021

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  4. MoClo Yeast Secretion Biosensor (YSB) Toolkit

    Plasmid Kit
    #1000000268
    Purpose
    Library of plasmids containing a variety of signal peptides, C-tags, and alpha-mating factor tags for use in protein secretion optimization in yeast.
    Depositor
    Joseph Brock
    Application
    Cloning and Synthetic Biology
    Vector Type
    Yeast Expression
    Cloning Type
    Golden Gate (MoClo)
    Available Since
    Sept. 17, 2025
    Availability
    Academic Institutions and Nonprofits only

  5. pAttPF3Hyg

    Plasmid
    #244929
    Purpose
    attP Hyg Resistant plasmid for RMI targeting an attB site
    Depositor
    Michael Nonet
    Article
    Nonet et al bioRxiv 2024.03.01.583017
    Type
    Empty backbone
    Use
    Phic31 recombinase mediated insertion (rmi)
    Expression
    Worm
    Promoter
    none
    Available Since
    Sept. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    244929-plasmid-map-sequence-id-486014

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  6. pAttBFHyg

    Plasmid
    #244926
    Purpose
    attB Hyg Resistant plasmid for RMI targeting an attP site
    Depositor
    Michael Nonet
    Article
    Nonet et al bioRxiv 2024.03.01.583017
    Type
    Empty backbone
    Use
    Phic31 recombinase mediated insertion (rmi)
    Expression
    Worm
    Promoter
    none
    Available Since
    Sept. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    244926-plasmid-map-sequence-id-486007

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  7. pBD_PYR1_156-F1

    Plasmid
    #241286
    Purpose
    Backbone for PYR1 sensor library assembly from oligopools; stop codon removed; PYR1 sensor library assembly from oligopools;
    Depositor
    Sean Cutler
    Type
    Empty backbone
    Expression
    Yeast
    Available Since
    Aug. 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    241286-plasmid-map-sequence-id-484590

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Showing: 2681 - 2700 of 5185 results