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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 15,952 results for: grna

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Showing: 2701 - 2720 of 15952 results

  2. pJH2972

    Plasmid
    #100956
    Purpose
    Cas9 driven by PGK1 promoter. gRNA can be cloned into the BplI sites. URA3MX marker
    Depositor
    James Haber
    Article
    Ranjith et al Protocol Exchange (2017)
    Type
    Empty backbone
    Use
    CRISPR and Synthetic Biology
    Expression
    Yeast
    Available Since
    Sept. 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    100956-plasmid-map-sequence-id-222978

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  5. pDIRECT_22D

    Plasmid
    #91136
    Purpose
    Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9_dead + CmYLCV:gRNAs with Csy4 spacers, Plant Selection: 2x35S:npt II
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    Engineering Reagent: 35S:Csy4-P2A-AtCas9_dead + CmYLCV:gRNAs with Csy4 spacers
    Use
    CRISPR
    Expression
    Plant
    Mutation
    D10A, H840A
    Available Since
    July 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    91136-plasmid-map-sequence-id-182475

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  8. pLenti spCas9 T2A mNeonGreen P2A puro

    Plasmid
    #122183
    Purpose
    The plasmid codes for a Flag-spCas9 protein, a mNeonGreen fluorescent protein and a puromycin resistance. The plasmid has two BsmBI acceptor sites to insert gRNA expressing sequences.
    Depositor
    Raphael Gaudin
    Inserts
    spCas9 (NEWENTRY )
    mNeonGreen
    Use
    Lentiviral
    Tags
    T2A
    Expression
    Mammalian
    Available Since
    April 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    122183-plasmid-map-sequence-id-241282

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  9. pAc3_gRNA1

    Plasmid
    #178746
    Purpose
    Expression of a gRNA that targets next to PAM library, used for A. chroococcum type I-E #3 system
    Depositor
    Chase Beisel
    Article
    Wimmer et al Mol Cell. 2022 Feb 16. pii: S1097-2765(2
    Insert
    gRNA that targets next to PAM library, used for A. chroococcum type I-E #3 system
    Expression
    Bacterial
    Available Since
    March 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    178746-plasmid-map-sequence-id-355198

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  10. pAc2_gRNA1

    Plasmid
    #178740
    Purpose
    Expression of a gRNA that targets next to PAM library, used for A. chroococcum type I-E #2 system
    Depositor
    Chase Beisel
    Article
    Wimmer et al Mol Cell. 2022 Feb 16. pii: S1097-2765(2
    Insert
    gRNA that targets next to PAM library, used for A. chroococcum type I-E #2 system
    Expression
    Bacterial
    Available Since
    March 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    178740-plasmid-map-sequence-id-355194

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  13. pDIRECT_23D

    Plasmid
    #91141
    Purpose
    Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9_dead + CmYLCV:gRNAs with Csy4 spacers, Plant Selection: 2x35S:bar
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    Engineering Reagent: 35S:Csy4-P2A-AtCas9_dead + CmYLCV:gRNAs with Csy4 spacers
    Use
    CRISPR
    Expression
    Plant
    Mutation
    D10A, H840A
    Available Since
    July 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    91141-plasmid-map-sequence-id-182269

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  14. pV1093

    Plasmid
    #111428
    Purpose
    CaCas9/gRNA Solo entry plasmid for cloning guides - contains stuffer with BsmBI sites - integrates at ENO1
    Depositor
    Gerald Fink
    Article
    Vyas et al Sci Adv. 2015;1(3):e1500248.
    Insert
    CaCas9/sgRNA-BsmBI stuffer
    Use
    CRISPR
    Expression
    Yeast
    Available Since
    Oct. 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    111428-plasmid-map-sequence-id-212463

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  16. pJH2971

    Plasmid
    #100955
    Purpose
    Cas9 driven by PGK1 promoter. gRNA can be cloned into the BplI sites. KANMX marker
    Depositor
    James Haber
    Article
    Ranjith et al Protocol Exchange (2017)
    Type
    Empty backbone
    Use
    CRISPR and Synthetic Biology
    Expression
    Yeast
    Available Since
    Sept. 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    100955-plasmid-map-sequence-id-193248

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Showing: 2701 - 2720 of 15952 results