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pJPC12-ΔM13-PRM-B0034-geneVI

Plasmid

#80858

Purpose

Accessory plasmid (encodes M13 gene VI)

Depositor

Mark Isalan

Article

Brodel et al Nat Commun. 2016 Dec 16;7:13858. doi: 10

Insert

M13 gene VI

Expression

Bacterial

Promoter

Lambda Prm

Available Since

Dec. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pSEVA47_Helper

Plasmid

#231420

Purpose

M13 helper genes cloned into the pSEVA631 vector. This plasmid does not carry an M13 packaging signal. (This plasmid is a gift of the Jaramillo Lab, where it is called PAJ296.)

Depositor

Alfonso Jaramillo

Article

Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

Insert

gp1-gp11

Use

Synthetic Biology

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pBS-iNOS

Plasmid

#19295

Depositor

Charles Lowenstein

Article

Lowenstein et al Proc Natl Acad Sci U S A. 1992 Aug 1. 89

Insert

inducible nitric oxide synthase (Nos2 Mouse)

Expression

Bacterial

Available Since

Nov. 21, 2008

Availability

Academic Institutions and Nonprofits only

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pJMD14

Plasmid

#138654

Purpose

phiOZJ integrating Streptomyces expression vector, intact RDF

Depositor

Joshua Blodgett

Article

Ko et al J Ind Microbiol Biotechnol. 2019 Nov 8.

Type

Empty backbone

Expression

Bacterial

Promoter

PermE* variant sequence from pDA1652

Available Since

April 29, 2020

Availability

Academic Institutions and Nonprofits only

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PAJ698

Plasmid

#178947

Purpose

Expresses P2 tail fiber (gpH) in fusion with S16 tail fiber (gp37)

Depositor

Alfonso Jaramillo

Article

Cunliffe et al ACS Synth Biol. 2022 Oct 21;11(10):3207-

Insert

Chimeric Tail Fiber Fusion P2-gpH and S16 gp37

Use

Unspecified

Expression

Bacterial

Available Since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pCRISPR-EASY

Plasmid

#241237

Purpose

CRISPR-Cas9 genome editing

Depositor

Tamara O'Connor

Article

Hossain et al PLoS One. 2024 Mar 14;19(3):e0299513. do

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pR_Lux

Plasmid

#226457

Purpose

lambda immunity region to the luciferase operon (lux)

Depositor

Emily Balskus

Article

Silpe et al Nature. 2022 Mar;603(7900):315-320. doi:

Insert

lambda immunity region to the luciferase operon (lux) from Photorhabdus luminescens

Expression

Bacterial

Available Since

Nov. 8, 2024

Availability

Academic Institutions and Nonprofits only

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fabp10a:ER-CreER; cryaa:mCerulean

Plasmid

#230077

Purpose

Expression of ERCreER in zebrafish hepatocytes.

Depositor

Sumeet Pal Singh

Article

Hovhannisyan et al NPJ Regen Med. 2026 Mar 30. doi: 10.1038

Insert

ERT2-Cre-ERT2

Use

Zebrafish expression

Promoter

fabp10a

Available Since

May 30, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pBYK01

Plasmid

#138656

Purpose

phiOZJ integrating Streptomyces expression vector lacking RDF

Depositor

Joshua Blodgett

Article

Ko et al J Ind Microbiol Biotechnol. 2019 Nov 8.

Type

Empty backbone

Expression

Bacterial

Promoter

PermE* variant sequence from pDA1652

Available Since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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pJMD5

Plasmid

#138652

Purpose

phiBT1 integrating Streptomyces expression vector

Depositor

Joshua Blodgett

Article

Ko et al J Ind Microbiol Biotechnol. 2019 Nov 8.

Type

Empty backbone

Expression

Bacterial

Promoter

PermE* variant sequence from pDA1652

Available Since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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pJMD13

Plasmid

#138653

Purpose

phiC31 integrating Streptomyces expression vector

Depositor

Joshua Blodgett

Article

Ko et al J Ind Microbiol Biotechnol. 2019 Nov 8.

Type

Empty backbone

Expression

Bacterial

Promoter

PermE* variant sequence from pDA1652

Available Since

June 10, 2020

Availability

Academic Institutions and Nonprofits only

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pME VIVIT NS

Plasmid

#188699

Purpose

Gateway middle element encoding the VIVIT peptide (no stop codon)

Depositor

David Tobin

Article

Brewer et al Cell Rep. 2022 Dec 13;41(11):111817. doi

Insert

VIVIT

Use

Synthetic Biology

Expression

Mammalian

Available Since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pQE-WT-CGG-R12-KIRV

Plasmid

#63668

Purpose

CGG-R12-KIRV RNAP mutant

Depositor

Andrew Ellington

Article

Meyer et al ACS Synth Biol. 2014 Oct 7.

Insert

CGG-R12-KIRV RNAP

Mutation

Q744K, L747V, N748H, L749I, R756E, L757M, H772R, …

Promoter

T5/Lac

Available Since

April 15, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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4A3.S'U'

Plasmid

#197676

Purpose

Expresses P1 S' tail fibre and U' chaperone

Depositor

Baojun Wang

Article

Huan et al J Mol Biol. 2022 Sep 15;434(21):167829.

Inserts

S'
U'

Expression

Bacterial

Promoter

Late S promoter, LPS and N/A

Available Since

June 12, 2023

Availability

Academic Institutions and Nonprofits only

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pQE-WT-CTGA-R13-AKSRV

Plasmid

#63622

Purpose

CTGA-R13-AKSRV RNAP mutant

Depositor

Andrew Ellington

Article

Meyer et al ACS Synth Biol. 2014 Oct 7.

Insert

CTGA-R13-AKSRV RNAP

Expression

Bacterial

Mutation

V725A, Q744K, L747I, N748S, R756T, Q758K, H772R, …

Promoter

T5/Lac

Available Since

July 29, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pLV VEET-GFP

Plasmid

#188706

Purpose

Lentivirus transfer plasmid encoding VEET-GFP

Depositor

David Tobin

Article

Brewer et al Cell Rep. 2022 Dec 13;41(11):111817. doi

Insert

VEET-GFP

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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p142

Plasmid

#8385

Depositor

Jeffrey Green

Article

Kaczmarczyk et al Nucleic Acids Res 2001 Jun 15;29(12):E56

Insert

LOX I/LOX II

Use

Cre/Lox

Tags

EGFP

Expression

Mammalian

Available Since

April 28, 2006

Availability

Academic Institutions and Nonprofits only
pT7_EcDRT3_3a-IntHis6

Plasmid

#255317

Purpose

Expresses EcDRT3 from Escherichia coli GF3-3 with an internal His6 tag on Drt3a

Depositor

Alex Gao

Article

Deng et al Science. 2026 Apr 16:eaed1656. doi: 10.1

Insert

EcDRT3 (Drt3a + Drt3b + ncRNA) with an internal His6 tag between Drt3a Thr247 and Glu248

Tags

His6

Expression

Bacterial

Available Since

May 18, 2026

Availability

Academic Institutions and Nonprofits only

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pLenti-EBFP-VAV1-W495L

Plasmid

#242839

Purpose

Encodes human VAV1 and N-terminal EBFP2 tag

Depositor

Cheng-han Yu

Article

Qi et al Cell Mol Life Sci. 2025 Mar 24;82(1):127

Insert

VAV1 (VAV1 Human)

Use

Lentiviral

Tags

EBFP2

Expression

Mammalian

Mutation

W495L

Available Since

Oct. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-EBFP2-VAV1-E201A

Plasmid

#242838

Purpose

Encodes human VAV1 and N-terminal EBFP2 tag

Depositor

Cheng-han Yu

Article

Qi et al Cell Mol Life Sci. 2025 Mar 24;82(1):127

Insert

VAV1 (VAV1 Human)

Use

Lentiviral

Tags

EBFP2

Expression

Mammalian

Mutation

E201A

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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