We narrowed to 9,753 results for: try

1. pENTR1A-3xFlag-M694V-MEFV

   Plasmid

   #167118

   Purpose

   Gateway entry plasmid with 3xFlag-pyrin (M694V/FMF variant)

   Depositor

   Thomas Henry

   Article

   Chirita et al Methods Mol Biol. 2022;2523:179-195. doi

   Insert

   MEFV (MEFV Human)
   

   Use

   Gateway entry vector

   Tags

   3xFlag

   Mutation

   M694V Methionine 694 to valine (FMF mutation)

   Available Since

   April 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. IF701: pMVP (L5-L4) APEX2

   Plasmid

   #121725

   Purpose

   pMVP L5-L4 entry plasmid, contains APEX2 for 4-component MultiSite Gateway Pro assembly. Allows fusion of N-term APEX2 to gene of interest.

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   APEX2 (open)
   

   Use

   Synthetic Biology; Pmvp gateway entry plasmid

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. LC501: pMVP (L3-L2) mCherry + polyA

   Plasmid

   #121765

   Purpose

   pMVP L3-L2 entry plasmid, contains mCherry + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term mCherry fusion to gene of interest.

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   mCherry + polyA
   

   Use

   Synthetic Biology; Pmvp gateway entry plasmid

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLew90β-GW/T7/PARP SAS/luciferase/Aldolase 3′UTR

   Plasmid

   #48157

   Purpose

   Transgenically expresses firefly luciferase protein in T. cruzi

   Depositor

   Rick Tarleton

   Article

   Canavaci et al PLoS Negl Trop Dis. 2010 Jul 13;4(7):e74

   Insert

   luciferase
   

   Use

   Luciferase; T. cruzi expression

   Promoter

   T7

   Available Since

   April 10, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. R10-3 /pQE-30

   Plasmid

   #79859

   Purpose

   The first mutant of the Aequorea victoria green fluorescent protein that forms a red chromophore.

   Depositor

   Konstantin Lukyanov

   Article

   Mishin et al Biochemistry. 2008 Apr 22;47(16):4666-73

   Insert

   R10-3
   

   Tags

   HisTag

   Expression

   Bacterial

   Mutation

   Aequorea victoria green fluorescent protein (avGF…

   Promoter

   T5 promoter / lac operator

   Available Since

   Sept. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEGFP-C1X-Nup93-mut

   Plasmid

   #87335

   Purpose

   To express human Nup93 in mammalian cells. It contains synonymous mutations that are not targeted by shRNA

   Depositor

   Lei Lu

   Article

   Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

   Insert

   NUP93 (NUP93 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   shRNA resistant changes

   Promoter

   CMV

   Available Since

   May 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. MAC-SARS-CoV-2 NSP8

   Plasmid

   #158359

   Purpose

   MAC-tagged gene expression

   Depositor

   Markku Varjosalo

   Article

   Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

   Insert

   SARS-CoV-2 NSP8 (ORF1ab )
   

   Tags

   MAC-tag

   Expression

   Mammalian

   Available Since

   Aug. 25, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. MAC-SARS-CoV-2 NSP10

   Plasmid

   #158361

   Purpose

   MAC-tagged gene expression

   Depositor

   Markku Varjosalo

   Article

   Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

   Insert

   SARS-CoV-2 NSP10 (ORF1ab )
   

   Tags

   MAC-tag

   Expression

   Mammalian

   Available Since

   Aug. 25, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pJCC_058 FnCas12a D917A

   Plasmid

   #171667

   Purpose

   For bacterial expression of FnCas12a D917A (RuvC-inactivating mutation) with a C-terminal sorting motif and His tag

   Depositor

   Jennifer Doudna

   Article

   Cofsky et al Elife. 2020 Jun 10;9. pii: 55143. doi: 1

   Insert

   FnCas12a D917A
   

   Tags

   6X His and sortase A sorting motif

   Expression

   Bacterial

   Mutation

   changed aspartate 917 to alanine

   Available Since

   July 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBad-CA TAG93

    Plasmid

    #105667

    Purpose

    expresses thermostable variant of human carbonic anhydrase II with TAG stop codon at site 93 and C-terminal 6x his tag

    Depositor

    Ryan Mehl

    Article

    Mehl et al 2020

    Insert

    human carbonic anhydrase II thermostable variant (CA2 Human)
    

    Tags

    6x his tag

    Expression

    Bacterial

    Mutation

    TAG stop codon at site 93

    Promoter

    pBad/arabinose

    Available Since

    March 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDONR221-SAR1

    Plasmid

    #67344

    Purpose

    hSar1 with stop codon in Gateway Entry vector

    Depositor

    Richard Kahn

    Article

    Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

    Insert

    SAR1 (SAR1A Human)
    

    Use

    Gateway entry vector

    Expression

    Bacterial

    Promoter

    None

    Available Since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

12. MAC-GPR78

    Plasmid

    #158382

    Purpose

    MAC-tagged gene expression

    Depositor

    Markku Varjosalo

    Article

    Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

    Insert

    GPR78 (GPR78 Human)
    

    Tags

    MAC-tag

    Expression

    Mammalian

    Available Since

    Aug. 25, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pEN_TT Tgfbr3-HA

    Plasmid

    #58497

    Purpose

    Gateway entry vector for an inducible HA-tagged mouse Tgfbr3

    Depositor

    Kevin Janes

    Article

    Wang et al Nat Cell Biol. 2014 Apr;16(4):345-56. do

    Insert

    Tgfbr3_HA (Tgfbr3 Mouse)
    

    Use

    Entry vector

    Tags

    HA

    Available Since

    Aug. 12, 2014

    Availability

    Academic Institutions and Nonprofits only

14. pEPQD1CB0770

    Plasmid

    #177060

    Purpose

    MoClo Level 1, position 5, transcriptional unit for transient expression of loganic acid O-methyltransferase (CrLAMT) from Catharanthus roseus driven by 35S promoter; contains a chloroplast transit peptide

    Depositor

    Nicola Patron

    Article

    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

    Insert

    loganic acid O-methyltransferase (CrLAMT) from Catharanthus roseus
    

    Use

    Synthetic Biology

    Available Since

    Dec. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pmalc4x_I499F_TEV

    Plasmid

    #47703

    Purpose

    Expresses MBP myocilin olfactomedin domain fusion protein with a TEV protease recognition sequence and the mutation I499F

    Depositor

    Raquel Lieberman

    Article

    Burns et al Biochemistry. 2011 Jul 5;50(26):5824-33.

    Insert

    Olfactomedin-like domain with I499F mutation (MYOC Human)
    

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    changed I499F

    Promoter

    Ptac

    Available Since

    Sept. 9, 2013

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pTRCHisB-TRF2_M

    Plasmid

    #50490

    Purpose

    E. Coli N-term HexHis Tagged Protein Expression Vector

    Depositor

    Jack Griffith

    Article

    Bower et al Biochemistry. 2014 Sep 2;53(34):5485-95.

    Insert

    TRF2 Myb (TERF2 Human)
    

    Tags

    6x His, T7 tag, and TEV protease site

    Expression

    Bacterial

    Mutation

    Contains amino acids 438-500

    Promoter

    pTrc

    Available Since

    Feb. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. KP201: pMVP (L5-L4) eGFP-P2A

    Plasmid

    #121708

    Purpose

    pMVP L5-L4 entry plasmid, contains eGFP-P2A for 4-component MultiSite Gateway Pro assembly. Allows expression of N-term eGFP linked by P2A to gene of interest.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    eGFP-P2A (open)
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pL106P-LUC-ENTR1A(w451-1)

    Plasmid

    #17455

    Purpose

    Gateway entry vector, Degron N-term Luciferase

    Depositor

    Paul Kaufman, Eric Campeau

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Insert

    FKBP-Luciferase fusion (MTOR firefly)
    

    Use

    Luciferase; Entry vector

    Tags

    FKBP12DD

    Available Since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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19. 459 pME-etv2

    Plasmid

    #49002

    Purpose

    full length coding sequence for zebrafish etv2 gene in gateway middle entry vector

    Depositor

    Nathan Lawson

    Article

    Moore et al Dev Biol. 2013 Sep 11. pii: S0012-1606(1

    Insert

    ets variant gene 2 (etsrp Zebrafish)
    

    Use

    Gateway middle entry

    Available Since

    Oct. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. KP301: pMVP (L5-L4) mCherry-P2A

    Plasmid

    #121710

    Purpose

    pMVP L5-L4 entry plasmid, contains mCherry-P2A for 4-component MultiSite Gateway Pro assembly. Allows expression of N-term mCherry linked by P2A to gene of interest.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    mCherry-P2A (open)
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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