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The development of a single molecule fluorescence standard and its application in estimating the stoichiometry of the nuclear pore complex.
Tie HC, Madugula V, Lu L
Biochem Biophys Res Commun. 2016 Sep 30;478(4):1694-9. doi: 10.1016/j.bbrc.2016.09.005. Epub 2016 Sep 6.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
87328pSUPER-retro-puro-shNup88-XbaITo express shRNA against human Nup88
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87329pSUPER-retro-puro-shNup88-HindIIITo express shRNA against human Nup88
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87330pSUPER-retro-puro-shNup93-XhoITo express shRNA against human Nup93
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87331pSUPER-retro-puro-shNup93-SpeIExpression of shRNA targeting at Nup93
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87332pSUPER-retro-puro-shNup35-EcoRITo express shRNA against human Nup35
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87333pSUPER-retro-puro-shNup133-2To express shRNA against human Nup133
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87334pSUPER-retro-puro-shNup133-1To express shRNA against human Nup133
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87335pEGFP-C1X-Nup93-mutTo express human Nup93 in mammalian cells. It contains synonymous mutations that are not targeted by shRNA
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87336pEGFP-C1X-Nup93To express human Nup93 in mammalian cells
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87337pEGFP-C1X-Nup88-mutTo express human Nup88 in mammalian cells. It contains synonymous mutations that are not targeted by shRNA
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87338pEGFP-C1X-Nup88To express human Nup88 in mammalian cells
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87339pEGFP-C1-KSENB-Nup133-mutTo express human Nup133 in mammalian cells. It contains synonymous mutations that are not targeted by shRNA
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87340pEGFP-C1-KSENB-Nup133To express human Nup133 in mammalian cells
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87341pEGFP-C1-Nup35-mutTo express human Nup35 in mammalian cells. It contains synonymous mutations that are not targeted by shRNA
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87342pEGFP-C1-Nup35To express human Nup35 in mammalian cells
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87343pEGFP-C1-6xHisTo express EGFP-6xHis in mammalian cell
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