We narrowed to 4,052 results for: gast

1. pAAV-Syn-AlstR-IRES2-EGFP

   Plasmid

   #14895

   Depositor

   Edward Callaway

   Article

   Tan et al Neuron. 2006 Jul 20. 51(2):157-70.

   Insert

   AlstR (AstA-R1 Fly)
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 11, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRSFDuet-1 IntS8 AA 1-308

   Plasmid

   #196905

   Purpose

   Expresses His-tagged Drosophila IntS8 amino acids 1-308

   Depositor

   Jeremy Wilusz

   Article

   Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

   Insert

   Integrator subunit 8 (IntS8 Fly)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. BII-ChBtW-ASCL2

   Plasmid

   #133375

   Purpose

   Piggybac vector for constitutive expression

   Depositor

   Blair Madison

   Article

   Fischer et al Am J Physiol Gastrointest Liver Physiol.

   Insert

   ASCL2 (ASCL2 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   CMV enhancer and hEf1a (CpG free)

   Available since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. BII-ChiNW-ASCL2

   Plasmid

   #133384

   Purpose

   Piggybac vector for constitutive expression

   Depositor

   Blair Madison

   Article

   Fischer et al Am J Physiol Gastrointest Liver Physiol.

   Insert

   ASCL2 (ASCL2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   CMV enhancer and hEf1a (CpG free)

   Available since

   Jan. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDropIn-T2A-split-intein-Gal4[1-20]-N-int

   Plasmid

   #199195

   Purpose

   Insert to create split-intein Gal4[N-int] knock-in vectors via Drop-In cloning, released via BsmBI digest

   Depositor

   Norbert Perrimon

   Article

   Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

   Insert

   T2A-split-intein-Gal4[1-20]-N-int, 3xP3-dsRed
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Aug. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAFW-Ago1

   Plasmid

   #50553

   Purpose

   Expression of FLAG tagged Ago1 in S2 cell

   Depositor

   Yukihide Tomari

   Article

   Kawamata et al Nat Struct Mol Biol. 2009 Sep;16(9):953-

   Insert

   Argonaute1 (AGO1 Fly)
   

   Use

   Tags

   FLAG

   Expression

   Insect

   Mutation

   Promoter

   Actin5C

   Available since

   Feb. 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUASp YFP Ras85D WT

   Plasmid

   #52242

   Purpose

   Drosophila expression of YFP-Ras85D

   Depositor

   Matthew Scott

   Insert

   Ras85D (Ras85D Fly)
   

   Use

   Drosophila

   Tags

   YFP

   Expression

   Insect

   Mutation

   Promoter

   GAL4

   Available since

   March 20, 2014

   Availability

   Academic Institutions and Nonprofits only

8. FLAG-Med29 pBacPAK8

   Plasmid

   #15369

   Depositor

   Ronald Conaway, Joan Conaway

   Article

   Sato et al J Biol Chem. 2003 Dec 12. 278(50):49671-

   Insert

   Med29 (MED29 Human)
   

   Use

   Baculovirus construction

   Tags

   FLAG

   Expression

   Mutation

   Promoter

   Available since

   Aug. 15, 2007

   Availability

   Academic Institutions and Nonprofits only

9. pGC(2.1)-U6.3

   Plasmid

   #170516

   Purpose

   PCR template vector for amplifying gRNAcore(2.1)-pU6.3 promoter fragment; used together with pAC-CR7T-gRNA2.1-nlsBFP

   Depositor

   Chun Han

   Article

   Koreman et al Proc Natl Acad Sci U S A. 2021 Apr 6;118

   Insert

   gRNAcore(2.1)-pU6.3
   

   Use

   Pcr template vector for amplifying grnacore(2.1)-…

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUASp YFP Ras85D DN

    Plasmid

    #52246

    Purpose

    Drosophila expression of YFP-Ras85D dominant negative mutant

    Depositor

    Matthew Scott

    Insert

    Ras85D (Ras85D Fly)
    

    Use

    Drosophila

    Tags

    YFP

    Expression

    Insect

    Mutation

    S17N; DN mutation

    Promoter

    GAL4

    Available since

    March 21, 2014

    Availability

    Academic Institutions and Nonprofits only

11. YFP Rab4DN

    Plasmid

    #99019

    Purpose

    Localization and visualization of the dominant negative form of Rab4

    Depositor

    Narasimhan Gautam

    Article

    O'Neill et al Dev Cell. 2018 Jun 15. pii: S1534-5807(1

    Insert

    Rab4 DN (Rab4 Fly, Synthetic)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    S22N Dominant negative

    Promoter

    CMV

    Available since

    June 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. FLAG-MED29

    Plasmid

    #15409

    Depositor

    Ronald Conaway, Joan Conaway

    Article

    Sato et al J Biol Chem. 2003 Dec 12. 278(50):49671-

    Insert

    MED29 (MED29 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    Available since

    Aug. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

13. K401-Micro

    Plasmid

    #122485

    Purpose

    Component of optically-induced motor dimerization system

    Depositor

    Matt Thomson

    Article

    Ross et al Nature. 2019 Aug;572(7768):224-229. doi:

    Insert

    His tag MBP Kinesin 1-401 SspB micro
    

    Use

    Tags

    H6-MBP-TEV and SspB micro

    Expression

    Bacterial

    Mutation

    Kinesin amino acids 1-401

    Promoter

    Available since

    Sept. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGEX-6P-2_dCrk

    Plasmid

    #131146

    Purpose

    Expresses GST::dCrk (based on crk isoform RA).

    Depositor

    Mark Peifer

    Article

    Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.

    Insert

    Crk (Crk Fly)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. OA-1045C

    Plasmid

    #125005

    Purpose

    Expresses gRNAs targeting hid, eve, and wingless

    Depositor

    Omar Akbari

    Article

    Buchman et al Nat Commun. 2021 Jun 2;12(1):3281. doi:

    Insert

    eve gRNA (eve Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    U6:3

    Available since

    Oct. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pT7-MS2-HA-C1-MBP-DmBam_13-36_AG

    Plasmid

    #148839

    Purpose

    Mammalian Expression of DmBam_13-36

    Depositor

    Elisa Izaurralde

    Article

    Sgromo et al RNA. 2018 Mar;24(3):381-395. doi: 10.126

    Insert

    DmBam_13-36 (bam Fly)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pUASTattB_NSnoFbox-vhhGFP4

    Plasmid

    #35578

    Depositor

    Markus Affolter

    Article

    Caussinus et al Nat Struct Mol Biol. 2011 Dec 11;19(1):1

    Insert

    NSnoFbox-vhhGFP4 (slmb Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    F box domain of slmb deleted.

    Promoter

    Available since

    April 3, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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18. pUAST-SIK3 T196E

    Plasmid

    #52975

    Purpose

    insect expression of SIK3 T196E constitutively active mutant

    Depositor

    Marc Montminy, Biao Wang

    Article

    Wang et al Cell. 2011 May 13;145(4):596-606. doi: 1

    Insert

    SIK3 (Sik3 Fly)
    

    Use

    Tags

    HA

    Expression

    Insect

    Mutation

    T196E constitutively active mutant

    Promoter

    Hsp70

    Available since

    May 20, 2014

    Availability

    Academic Institutions and Nonprofits only

19. pAFMW-Nbr-D435A/E437A

    Plasmid

    #40829

    Purpose

    Expresses Flag, Myc tagged mutant Nbr in Drosophila cells

    Depositor

    Phillip Zamore

    Article

    Han et al Curr Biol. 2011 Nov 22;21(22):1878-87. E

    Insert

    Nbr D435A/E437A (Nbr Fly)
    

    Use

    Tags

    3xFLAG and 6xMyc

    Expression

    Insect

    Mutation

    D435A/E437A, part of the DEDD motif; mutations p…

    Promoter

    Actin5C

    Available since

    Sept. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

20. 1098A=TI-pgSIT[sxl,bTub,Hasp70Bb-Cas9]

    Plasmid

    #149426

    Purpose

    The attB plasmid with the mini-white harboring two U6.3-gRNAs targeting Dmel sxl and bTub genes, and Hsp70Bb-Cas9-T2A-eGFP-p10.

    Depositor

    Omar Akbari

    Article

    Kandul et al CRISPR J. 2021 Dec;4(6):822-835. doi: 10

    Inserts

    U6.3-gRNAs[Sxl, bTub]
    Hsp70Bb-Cas9-T2A-eGFP-p10
    

    Use

    CRISPR

    Tags

    eGFP

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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