We narrowed to 6,714 results for: hwa

1. pLX317-hcRED

   Plasmid

   #115440

   Purpose

   Constitutive lentiviral expression

   Depositor

   William Hahn

   Article

   Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

   Insert

   hcRED
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Dec. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCarCBADE_ProB(I69E)A

   Plasmid

   #117756

   Purpose

   ProB(I69E)A and codon optimized CarCBADE enzymes (in that order) in pBbaA5k backbone

   Depositor

   Greg Bokinsky

   Article

   Shomar et al Nat Chem Biol. 2018 Aug;14(8):794-800. d

   Insert

   Glutamate kinase (ProA), mutant pyrroline-5-carboxylate synthase (ProBI69E) from E. coli and codon optimised CarCBADE operon
   

   Expression

   Bacterial

   Promoter

   PLac(UV5)

   Available Since

   Dec. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRevTRE MKL1-N100

   Plasmid

   #19848

   Depositor

   Ron Prywes

   Article

   Muehlich et al Mol Cell Biol. 2008 Oct;28(20):6302-13.

   Insert

   MKL1-N100 (MRTFA Human)
   

   Use

   Retroviral

   Tags

   3xFLAG

   Mutation

   deleted amino acids 1-100

   Available Since

   Jan. 13, 2009

   Availability

   Academic Institutions and Nonprofits only

4. pQE80L-SH3

   Plasmid

   #160331

   Purpose

   This plasmid encodes an engineered oxygen-independent flavin binding fluorescent protein with N-terminal 6xHis tag. Its thermostability and quantum yield is improved compared to wild type.

   Depositor

   Sang Taek Jung

   Article

   Ko et al J Agric Food Chem. 2019 Oct 30;67(43):12

   Insert

   SH3
   

   Tags

   6xHis tag

   Expression

   Bacterial

   Promoter

   T5

   Available Since

   March 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBabe(puro) mouse Egr3

   Plasmid

   #108102

   Purpose

   Retroviral vector for expression of mouse Egr3

   Depositor

   Peter Johnson

   Article

   Salotti et al Mol Cell Biol. 2015 Mar;35(5):866-83. do

   Insert

   Egr3 (Egr3 Mouse)
   

   Use

   Retroviral

   Expression

   Mammalian

   Promoter

   SV40

   Available Since

   April 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRSFDuet-1_VNp15-StefinA

   Plasmid

   #182405

   Purpose

   Bacterial expression of Vesicle Nucleating peptide15-stefinA fusion

   Depositor

   Dan Mulvihill

   Article

   Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

   Insert

   VNp15-stefin (CSTA Human)
   

   Tags

   Vesicle Nucleating peptide 15 (VNp15)

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCVL.Active/Repressed TLR (Sce)

   Plasmid

   #45575

   Purpose

   Codes for the Active/Repressed TLR with the target site for I-Sce I nuclease

   Depositor

   Andrew Scharenberg

   Article

   Kuhar et al Nucleic Acids Res. 2013 Oct 10.

   Inserts

   eGFP with I-Sce I TS
   +3 mCherry
   

   Use

   Lentiviral

   Tags

   T2A dislinker and iRFP

   Expression

   Bacterial and Mammalian

   Mutation

   embedded I-Sce I TS from 163-185

   Promoter

   SFFV

   Available Since

   Nov. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. THOC1

   Plasmid

   #156081

   Purpose

   For use in RBP tethering screen

   Depositor

   Eugene Yeo

   Article

   Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

   Insert

   THOC1 (THOC1 Human)
   

   Tags

   V5-MCP

   Expression

   Mammalian

   Mutation

   none

   Promoter

   EF-1a

   Available Since

   Nov. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCDNA3 Flag MKK4(ala)

   Plasmid

   #15314

   Depositor

   Roger Davis

   Article

   Whitmarsh et al Science. 1995 Jul 21. 269(5222):403-7.

   Insert

   MKK4(ala) (MAP2K4 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Ser-220Ala Thr-224Ala

   Available Since

   July 25, 2007

   Availability

   Academic Institutions and Nonprofits only

10. 3341 pcDNA3 Bcl-2 T69A S70A S87A

    Plasmid

    #13340

    Depositor

    Stanley Korsmeyer

    Article

    Yamamoto et al Mol Cell Biol 1999 Dec;19(12):8469-78.

    Insert

    Bcl-2 (BCL2 Human)
    

    Expression

    Mammalian

    Mutation

    changed Threonine 69 to Alanine; changed Serine 7…

    Available Since

    Nov. 9, 2006

    Availability

    Academic Institutions and Nonprofits only

11. SR140

    Plasmid

    #156037

    Purpose

    For use in RBP tethering screen

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    SR140 (U2SURP Human)
    

    Tags

    V5-MCP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    EF-1a

    Available Since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pQlinkH-PTPIP51(36-235)

    Plasmid

    #170533

    Purpose

    Expresses Cleavable His-tagged PTPIP51_ΔTPR domain (36-235) in E. Coli

    Depositor

    Byung Il Lee

    Article

    Yeo et al EMBO Rep. 2021 Jun 4;22(6):e51323. doi:

    Insert

    Protein tyrosine phosphatase interacting protein 51 (RMDN3 Human)
    

    Tags

    His tag

    Expression

    Bacterial

    Promoter

    T5 promoter

    Available Since

    Aug. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRK5-FLAG-Sestrin3

    Plasmid

    #72592

    Purpose

    overexpression, codon optimized for mammalian expression

    Depositor

    David Sabatini

    Article

    Chantranupong et al Cell Rep. 2014 Oct 9;9(1):1-8. doi: 10.1

    Insert

    SESN3 (SESN3 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    codon optimized for mammalian expression, see dep…

    Promoter

    CMV

    Available Since

    March 28, 2016

    Availability

    Academic Institutions and Nonprofits only

14. CABE-T2.19

    Plasmid

    #193265

    Purpose

    Expression of CABE-T2.19 base editor in mammalian cells, CMV promoter

    Depositor

    Nicole Gaudelli

    Article

    Lam et al Nat Biotechnol. 2023 Jan 9. doi: 10.1038

    Insert

    CABE-T2.19
    

    Expression

    Mammalian

    Mutation

    D10A (Cas9), TadA mutations within

    Promoter

    CMV

    Available Since

    Feb. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. YF-Cdc42(CA)

    Plasmid

    #20152

    Depositor

    Tobias Meyer

    Article

    Inoue et al Nat Methods. 2005 Jun . 2(6):415-8.

    Insert

    YFP-FKBP-Cdc42(CA) (CDC42 Human)
    

    Expression

    Mammalian

    Mutation

    Cdc42H was inserted into the multiple cloning sit…

    Available Since

    March 26, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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16. TTR C10S S85C

    Plasmid

    #184410

    Purpose

    bacterial expression of TTR

    Depositor

    Peter Wright

    Article

    Sun et al Proc Natl Acad Sci U S A. 2018 Jul 3;115

    Insert

    TTR (TTR Human)
    

    Expression

    Bacterial

    Mutation

    C10S, S85C

    Promoter

    T7

    Available Since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV5 MEKK2 WT

    Plasmid

    #12182

    Depositor

    Gary Johnson

    Article

    Blank et al J Biol Chem. 1996 Mar 8. 271(10):5361-8.

    Insert

    MEKK2 (Map3k2 Mouse)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    Sept. 22, 2006

    Availability

    Academic Institutions and Nonprofits only

18. pCMV5 MEKK4alpha WT

    Plasmid

    #12187

    Depositor

    Gary Johnson

    Article

    Gerwins et al J Biol Chem. 1997 Mar 28. 272(13):8288-9

    Insert

    MEKK4 (Map3k4 Mouse)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    Sept. 22, 2006

    Availability

    Academic Institutions and Nonprofits only

19. pCMV-flag Mst2 (KR)

    Plasmid

    #27372

    Depositor

    Kunliang Guan

    Article

    Zhao et al Genes Dev. 2007 Nov 1. 21(21):2747-61.

    Insert

    Mst2 (STK3 Human)
    

    Tags

    flag

    Expression

    Mammalian

    Mutation

    K56R (catalytically inactive)

    Available Since

    Feb. 2, 2011

    Availability

    Academic Institutions and Nonprofits only

20. pLXSN-H-Ras_N17

    Plasmid

    #39537

    Depositor

    Julian Downward

    Article

    Rodriguez-Viciana et al Cell. 1997 May 2;89(3):457-67.

    Insert

    HRAS N17 (HRAS Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    N17

    Available Since

    Aug. 15, 2012

    Availability

    Academic Institutions and Nonprofits only