We narrowed to 22,436 results for: Sele

1. pEBTetDBl-CLIP-CP110

   Plasmid

   #136852

   Purpose

   Mammalian expression of the centrosomal protein Cep110 N-terminally fused to CLIP-tag

   Depositor

   Kai Johnsson

   Article

   Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

   Insert

   CLIP-CP110 (CCP110 Synthetic, Human)
   

   Use

   Tags

   FLAG-tag / His-tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV-TetO2

   Available since

   April 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pETDuet-1-6xHis-TEV-Nsp8

   Plasmid

   #201022

   Purpose

   Bacterial expression of codon optimized SARS-CoV-2 nsp8 tagged with an N-terminus His-tag followed by a TEV protease cleavage site.

   Depositor

   Sharon Rozovsky

   Article

   Ghelichkhani et al J Mol Biol. 2023 Apr 15;435(8):168008. d

   Insert

   non-structural protein 8 (ORF1ab Synthetic, Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   6xHis-TEV

   Expression

   Bacterial

   Mutation

   Gene insert is codon optimized for Ecoli.

   Promoter

   T7/LacO

   Available since

   May 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCEP4-myc-HLA-A2-VN

   Plasmid

   #135485

   Purpose

   Mammalian expression of VN-fused and myc-tagged HLA-A*02:01

   Depositor

   Erik Procko

   Article

   McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

   Insert

   HLA-A*02:01 (HLA-A Human)
   

   Use

   Tags

   Extracellular c-myc epitope tag, Signal peptide f…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-hGRM1-RFP

   Plasmid

   #22920

   Depositor

   Edward Callaway

   Article

   Nathanson et al Front Neural Circuits. 2009 . 3():19.

   Insert

   human receptor metabotropic 1 promoter driving DsRedExpress (GRM1 Human)
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 25, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-mA93-RFP

   Plasmid

   #22918

   Depositor

   Edward Callaway

   Article

   Nathanson et al Front Neural Circuits. 2009 . 3():19.

   Insert

   mouse riken gene A930038C07Rik promoter driving DsRedExpress (A930038C07Rik Mouse)
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 26, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDS170-enNTS1

   Plasmid

   #199152

   Purpose

   JZ73, bacterial expression construct expressing thermostabilised rat neurotensin receptor 1 (enNTS1)

   Depositor

   Daniel Scott

   Article

   Bumbak et al Cell Rep. 2023 Jan 31;42(1):112015. doi:

   Insert

   enNTS1 (Ntsr1 Rat)
   

   Use

   Tags

   10xHis tag, Avi tag, MBP, and muGFP

   Expression

   Bacterial

   Mutation

   V208M, C273S, C391S and C393S (over NTS1-B5)

   Promoter

   Lac/T5

   Available since

   May 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLG1-puro-sgULK1-1

   Plasmid

   #109004

   Purpose

   CRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)

   Depositor

   Jacob Corn

   Article

   Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

   Insert

   ULK1-sgRNA #1 (ULK1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mU6 promoter

   Available since

   Aug. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pYPQ131-STU-As

   Plasmid

   #138094

   Purpose

   Golden Gate entry vector for 1st crRNA cloning with AsCas12a crRNA scaffold flanked by HH and HDV ribozymes

   Depositor

   Yiping Qi

   Article

   Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   March 31, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEBTetBl-CLIP-CP110

   Plasmid

   #136851

   Purpose

   Mammalian expression of the centrosomal protein Cep110 N-terminally fused to CLIP-tag

   Depositor

   Kai Johnsson

   Article

   Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

   Insert

   CLIP-CP110 (CCP110 Synthetic, Human)
   

   Use

   Tags

   FLAG-tag / His-tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV-TetO2

   Available since

   April 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAAV-CAGGS-Flex-Turbo/GFP

    Plasmid

    #197886

    Purpose

    Can be used to generate AAV virus that will express Turbo/GFP in the presence of Cre

    Depositor

    Kazuto Kobayashi

    Article

    Matsushita et al Cell Rep Methods. 2023 Jan 18;3(2):10039

    Insert

    Turbo/GFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    N/A

    Promoter

    CAG

    Available since

    March 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEBTetBl-CLIP-Centrobin

    Plasmid

    #136878

    Purpose

    Mammalian expression of the centrosomal protein Centrombin N-terminally fused to CLIP-tag

    Depositor

    Kai Johnsson

    Article

    Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

    Insert

    CLIP-Centrobin (CNTROB Synthetic, Human)
    

    Use

    Tags

    FLAG-tag / His-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TetO2

    Available since

    March 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pQC hKGA.wt V5-His IRES G418

    Plasmid

    #110390

    Purpose

    γ-Retroviral transfer vector for expressing glutminase isoform KGA (wild type), C-terminal V5-6xHis tags, IRES-driven Geneticin selection.

    Depositor

    Sandra Martha Gomes Dias

    Insert

    KGA glutaminase (GLS Human)
    

    Use

    Retroviral

    Tags

    6xHis and V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEBTetD-SNAP-Centrin2

    Plasmid

    #136785

    Purpose

    Mammalian expression of the centrosomal protein Centrin2 N-terminally fused to SNAP-tag

    Depositor

    Kai Johnsson

    Article

    Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

    Insert

    SNAP-Centrin2 (CETN2 Synthetic, Human)
    

    Use

    Tags

    FLAG-tag / His-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TetO2

    Available since

    March 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEBTet-SNAP-Cep164

    Plasmid

    #136824

    Purpose

    Mammalian expression of the centrosomal protein Cep164 N-terminally fused to SNAP-tag

    Depositor

    Kai Johnsson

    Article

    Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

    Insert

    SNAP-Cep164 (CEP164 Synthetic, Human)
    

    Use

    Tags

    FLAG-tag / His-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TetO2

    Available since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pEBTet-SNAP-KIAA0841

    Plasmid

    #136810

    Purpose

    Mammalian expression of the centrosomal protein KIAA0841 N-terminally fused to SNAP-tag

    Depositor

    Kai Johnsson

    Article

    Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

    Insert

    SNAP-KIAA0841 (HAUS5 Synthetic, Human)
    

    Use

    Tags

    FLAG-tag / His-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TetO2

    Available since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pEBTet-SNAP-Centrin2

    Plasmid

    #136784

    Purpose

    Mammalian expression of the centrosomal protein Centrin2 N-terminally fused to SNAP-tag

    Depositor

    Kai Johnsson

    Article

    Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

    Insert

    SNAP-Centrin2 (CETN2 Synthetic, Human)
    

    Use

    Tags

    FLAG-tag / His-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TetO2

    Available since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. HOXB1 (human) HIS-tag pET

    Plasmid

    #8520

    Depositor

    Corey Largman

    Article

    Shen et al Nucleic Acids Res 1996 Mar 1;24(5):898-9

    Insert

    HOXB1 (HOXB1 Human)
    

    Use

    Tags

    His and T7

    Expression

    Bacterial

    Mutation

    PCR from cDNA clone

    Promoter

    Available since

    July 6, 2006

    Availability

    Academic Institutions and Nonprofits only

18. pJT131_GalL_cCDA1-NGBE3

    Plasmid

    #145095

    Purpose

    Expressing base editor cCDA1-NGBE3 in yeast cells

    Depositor

    Ralph Bock

    Article

    Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

    Insert

    cCDA1-NGBE3
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    spCas9(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1…

    Promoter

    GalL

    Available since

    July 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLenti-161K enhancer-ETS-CORE-MUT-minP:fLuc-TK:rLuc

    Plasmid

    #138367

    Purpose

    Lentiviral plasmid contains 161k modified human enhancer with with ETS binding sites mutated driving firefly Luciferase 2 and Renilla Luciferase driven by TK promoter

    Depositor

    Nathan Lawson

    Article

    Sissaoui et al Circ Res. 2020 Feb 17. doi: 10.1161/CIRC

    Insert

    161K human enhancer, with ETS binding sites mutated, driving firefly enhancer and TK driving refills luciferase
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCEP4-myc-HLA-A3-VN

    Plasmid

    #135506

    Purpose

    Mammalian expression of VN-fused and myc-tagged HLA-A*03:01

    Depositor

    Erik Procko

    Article

    McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

    Insert

    HLA-A*03:01 (HLA-A Human)
    

    Use

    Tags

    Extracellular c-myc epitope tag, Signal peptide f…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge