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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 4,072 results for: golden gate

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Showing: 2881 - 2900 of 4072 results

  6. PB-CT3G-ERP2

    Plasmid
    #175497
    Purpose
    All-in-one piggyBac transposon vector for mCMV+dox-inducible expression of Golden Gate cloned elements (hEF1a-driven rtTA and puromycin resistance)
    Depositor
    George Church
    Article
    Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    TRE3G-mCMV
    Available Since
    Oct. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    175497-plasmid-map-sequence-id-345451

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  9. pShHELIX_sgRNA_entry (CJT32)

    Plasmid
    #181782
    Purpose
    Expresses ShHELIX containing a nicking I-AniI fusion to ShTnsB. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).
    Depositor
    Benjamin Kleinstiver
    Article
    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Insert
    nAniI-ShTnsB, ShTnsC, ShTniQ, ShCas12k
    Expression
    Bacterial
    Mutation
    nAniI = K227M, F80K, L232K
    Promoter
    Lac and J23119
    Available Since
    Feb. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    181782-plasmid-map-sequence-id-354808

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  12. pRAM28

    Plasmid
    #234314
    Purpose
    pColE1 origin Cat-RNA U1376, Multiplexing ribozyme splicing reactions for the purpose of comparing host range of origins of replications
    Depositor
    James Chappell
    Article
    Kalvapalle et al Nat Biotechnol. 2025 Mar 18. doi: 10.103
    Insert
    U1367 Targeting RAM, Barcode 2
    Use
    Synthetic Biology
    Promoter
    P. CymRC
    Available Since
    March 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    234314-plasmid-map-sequence-id-466009

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  13. pC-GoldyTALEN

    Plasmid
    #38143
    Purpose
    destination vector for the Golden Gate TALEN kit, directs expression of TALENs from a truncated CAGs promoter
    Depositor
    Daniel Carlson
    Article
    Carlson et al Proc Natl Acad Sci U S A. 2012 Oct 1.
    Insert
    GoldyTALEN
    Tags
    AcV5 and FokI homodimer
    Expression
    Mammalian
    Mutation
    Truncate at N and C terminous
    Promoter
    miniCaggs
    Available Since
    July 30, 2012
    Availability
    Academic Institutions and Nonprofits only
    38143-plasmid-map-sequence-id-48254

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Showing: 2881 - 2900 of 4072 results