We narrowed to 4,698 results for: golden gate
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Plasmid#117530PurposeLevel0 Golden Gate part: CDS, Sp-xCas9 3.7 (no stop codon)DepositorInsertSp-xCas9 3.7 (no stop codon)
UseCRISPRMutationA262T, R324L, S409I, E480K, E543D, M694I, E1219VAvailable SinceNov. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEB0_11_5xQUAS_CYC1cp
Plasmid#236215PurposePlasmid encoding 5xQUAS_CYC1cp (five repeats of the binding sequence of QF directly upstream of the CYC1 core promoter) as a Type 2 part to be used in the Dueber Yeast MoClo Toolkit.DepositorInsertCYC1 core promoter with 5x QUAS binding sites
UseSynthetic BiologyExpressionBacterialAvailable SinceMarch 19, 2026AvailabilityAcademic Institutions and Nonprofits only -
M_3HN5SR
Plasmid#249741PurposeMinimal synthetic construct for assessing speckle localization containing a 3' splice site, an HNRNP motif-rich region, 5' splice site, and an SR motif-rich regionDepositorInsertM_3HN5SR
ExpressionMammalianPromoterTRE+CMVAvailable SinceMarch 11, 2026AvailabilityAcademic Institutions and Nonprofits only -
M_3SR5HN
Plasmid#249737PurposeMinimal synthetic construct for assessing speckle localization containing a 3' splice site, SR motif-rich region, 5' splice site, and an HNRNP motif-rich regionDepositorInsertM_3SR5HN
ExpressionMammalianPromoterTRE+CMVAvailable SinceMarch 10, 2026AvailabilityAcademic Institutions and Nonprofits only -
BeYDV-RT-pUPD2
Plasmid#240747PurposeBeYDV viral vector containing a repair template.DepositorInsertBeYDV:RT
UseSynthetic BiologyAvailable SinceFeb. 2, 2026AvailabilityAcademic Institutions and Nonprofits only -
BCTV-CS-pUPD2
Plasmid#240746PurposeBCTV complementary sense ORFs with C1 Goldenbraid grammarDepositorInsertBCTV CS
UseSynthetic BiologyAvailable SinceFeb. 2, 2026AvailabilityAcademic Institutions and Nonprofits only -
BCTVΔIR-pUPD2
Plasmid#240717PurposeBCTV complementary sense ORFs lacking the IR. Has C1 Goldenbraid grammar.DepositorInsertBCTV CS
UseSynthetic BiologyAvailable SinceFeb. 2, 2026AvailabilityAcademic Institutions and Nonprofits only -
BCTVΔVS-pUPD2
Plasmid#240718PurposeBCTV IR lacking virion sense ORFs.DepositorInsertBCTV IR
UseSynthetic BiologyAvailable SinceFeb. 2, 2026AvailabilityAcademic Institutions and Nonprofits only -
RT B2-B6-pUPD2
Plasmid#240719PurposeGUS:NPTII repair template used for HDR experiments with compatible reporter lines. Has B2-B6 Goldenbraid grammar.DepositorInsertRT
UseSynthetic BiologyAvailable SinceFeb. 2, 2026AvailabilityAcademic Institutions and Nonprofits only -
RT B6-pUPD2
Plasmid#240720PurposeGUS:NPTII repair template used for HDR experiments with compatible reporter lines. Has B6 Goldenbraid grammar.DepositorInsertRT
UseSynthetic BiologyAvailable SinceFeb. 2, 2026AvailabilityAcademic Institutions and Nonprofits only -
ZFN-pUPD2
Plasmid#240721PurposeZFN that targets the broken GUS:NPTII reporter with B2-B5 Goldenbraid grammar.DepositorInsertZFN
UseSynthetic BiologyAvailable SinceFeb. 2, 2026AvailabilityAcademic Institutions and Nonprofits only -
BCTV-YPET-pDGB3alpha2
Plasmid#240722PurposeBCTV vector for YPET expression.DepositorInsertYPET
ExpressionPlantAvailable SinceFeb. 2, 2026AvailabilityAcademic Institutions and Nonprofits only -
BCTVΔVS-YPET-pDGB3alpha1
Plasmid#240723PurposeBCTV vector for YPET expression. Lacks the BCTV virion sense ORFs.DepositorInsertYPET
ExpressionPlantAvailable SinceFeb. 2, 2026AvailabilityAcademic Institutions and Nonprofits only -
BCTV-RT-pDGB3alpha2
Plasmid#240724PurposeBCTV vector containing the GUS:NPTII repair template for HDR experiments.DepositorInsertRT
ExpressionPlantAvailable SinceFeb. 2, 2026AvailabilityAcademic Institutions and Nonprofits only -
pKG0893_pHarbor-LentiX1_backbone
Plasmid#239704PurposePaqCI Golden Gate assembly plasmid encoding the LentiX1 backboneDepositorTypeEmpty backboneUseSynthetic BiologyAvailable SinceJan. 28, 2026AvailabilityAcademic Institutions and Nonprofits only -
pTL52_pyCAG-s36GFPHaloTag-IRES-mCherry3NLS-polyA
Plasmid#223544PurposeMinimal PUFFHalo cassette with s36GFP-HaloTag and mCherry-3NLS, strong constitutive promoter CAG, no insulation or mammalian selection geneDepositorInserts36GFP-HaloTag, mCherry-3NLS
ExpressionMammalianPromoterCAGAvailable SinceJan. 21, 2026AvailabilityAcademic Institutions and Nonprofits only -
CAG-rtTA-2A-tagBFP-bGH_pKG3264
Plasmid#246372PurposeExpression plasmid for rtTA-2A-tagBFP driven from the CAG promoterDepositorInsertrtTA
UseSynthetic BiologyExpressionMammalianMutationnoneAvailable SinceNov. 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
EFS-rtTA-2A-mRuby2-bGH_pKG2064
Plasmid#246373PurposeExpression plasmid for rtTA-2A-mRuby2 driven from the EFS promoterDepositorInsertrtTA
UseSynthetic BiologyExpressionMammalianMutationnoneAvailable SinceNov. 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
EF1a-iRFP670-SV40_pKG726
Plasmid#246374PurposeExpression plasmid for iRFP670 driven from the EF1a promoter. Co-transfection marker for transfection experiments.DepositorInsertiRFP670
UseSynthetic BiologyExpressionMammalianMutationnoneAvailable SinceNov. 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGMF2-D
Plasmid#242206PurposeAtU6-26p::sgRNA scaffold in L1 vector backbone, for subcloning of 2nd 20 bp target sequence. For use in dicots.DepositorInsertLevel1 AtU6-26p::sgRNA2 for subcloning of 20 bp target sequence
UseCRISPR and Synthetic BiologyExpressionPlantPromoterAtU6-26pAvailable SinceNov. 10, 2025AvailabilityAcademic Institutions and Nonprofits only