We narrowed to 1,108 results for: pFAST

1. pFastBac-Gm129-Flag

   Plasmid

   #53766

   Purpose

   baculovirus expression of Gm129

   Depositor

   Aziz Sancar

   Article

   Annayev et al J Biol Chem. 2014 Feb 21;289(8):5013-24.

   Insert

   Gm129 (Ciart Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Insect

   Mutation

   Promoter

   Polyhedrin

   Available since

   Sept. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

2. pFastBac Dual α1B/β3 Tubulin

   Plasmid

   #170552

   Purpose

   Encodes codon-optimized human α1B and β3 tubulin for expression in and purification from insect cells.

   Depositor

   Tarun Kapoor

   Article

   Ti et al Dev Cell. 2018 Oct 22;47(2):175-190.e5.

   Inserts

   TUBA1B (TUBA1B Human)
   TUBB3 (TUBB3 Human)
   

   Use

   Tags

   AP linker, GS linker (GGSGG), His10 tag, L21 enha…

   Expression

   Insect

   Mutation

   Promoter

   Available since

   June 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFastBac Dual wt p110α/p85α

   Plasmid

   #199302

   Purpose

   co-expression of His-tagged p110α wt and Avi-tagged p85α wt proteins

   Depositor

   Peter Vogt

   Article

   Favalli et al Nat Chem. 2021 Jul;13(7):714. doi: 10.10

   Insert

   His-tagged p110α wt and Avi-tagged p85α wt (PIK3CA, PIK3R1 Human)
   

   Use

   Tags

   Avi and His

   Expression

   Insect

   Mutation

   Promoter

   Available since

   June 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pFastBac_HT_B_6xHis-TEV-GFP-NBR1

   Plasmid

   #190928

   Purpose

   Plasmid for the expression and purification of GFP labelled NBR1. Internal reference: SMC 914

   Depositor

   Sascha Martens

   Article

   Turco et al Nat Commun. 2021 Sep 1;12(1):5212. doi:

   Insert

   NBR1 (NBR1 Human)
   

   Use

   Tags

   Strep-TEV-GFP

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFastBac1-OSF-TRIM5alpha(human)

   Plasmid

   #79034

   Purpose

   Baculoviral entry vector to produce Bac-to-bac baculoviruses

   Depositor

   Wesley Sundquist

   Article

   Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

   Insert

   OSF-TRIM5alpha(human) (TRIM5 Human)
   

   Use

   Tags

   OneSTrEP-FLAG

   Expression

   Insect

   Mutation

   Promoter

   polyhedrin

   Available since

   March 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFastBac LIC cloning vector (4A)

   Plasmid

   #30111

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   June 9, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pFastBac-GST-DAXX-His

   Plasmid

   #175781

   Purpose

   DAXX protein for baculovirus expression system

   Depositor

   Xiaolu Yang

   Article

   Huang et al Nature. 2021 Aug 18. pii: 10.1038/s41586

   Insert

   death domain associated protein (DAXX Human)
   

   Use

   Tags

   GST and TEV

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Oct. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pFastbac-Dual-StrepII-BARD1

   Plasmid

   #137166

   Purpose

   Expresses human StrepII-tagged BARD1 for purification from baculovirus/insect cell system

   Depositor

   Andrew Deans

   Article

   Tan et al PLoS One. 2020 Feb 24;15(2):e0229000. do

   Insert

   BARD1 (BARD1 Human)
   

   Use

   Tags

   StrepII

   Expression

   Insect

   Mutation

   fully synthetic, codon optimized for insect expre…

   Promoter

   p10

   Available since

   April 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pFastbacHTB HA-human PP2A Cα D88N

   Plasmid

   #132633

   Purpose

   expresses HA-human PP2A Cα D88N in Hi5 insect cell

   Depositor

   Wenqing Xu

   Article

   Cho et al Nature. 2007 Jan 4;445(7123):53-7. doi:

   Insert

   PP2A Cα D88N
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Oct. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pFastBac (GST-SNAP-Shp2)

    Plasmid

    #177897

    Purpose

    Baculo expression of GST tag fused to SNAP tag and human Shp2

    Depositor

    Ron Vale

    Article

    Hui et al Science. 2017 Mar 31;355(6332):1428-1433

    Insert

    Shp2 (PTPN11 Human)
    

    Use

    Tags

    TEV-GST-PreScission cut site-SNAP tag

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin

    Available since

    Dec. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pFastBac(His10-SNAP-Csk)

    Plasmid

    #177895

    Purpose

    Baculo expression of His10 tag fused to SNAPtag and human Csk

    Depositor

    Ron Vale

    Article

    Hui et al Nat Struct Mol Biol. 2014 Feb;21(2):133-

    Insert

    Csk (CSK Human)
    

    Use

    Tags

    10xHis-TEV-TVMV-SNAP tag

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin

    Available since

    Oct. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pFastBac HTB DPP9S S730A

    Plasmid

    #169946

    Purpose

    Purification of catalytically-dead DPP9S from insect cells

    Depositor

    Hao Wu

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    DPP9 (DPP9 Human)
    

    Use

    Tags

    HIS-TEV

    Expression

    Insect

    Mutation

    S730A

    Promoter

    polyhedrin

    Available since

    May 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pFastBac_Dual_GST-TEV-EGFP-TBK1

    Plasmid

    #187830

    Purpose

    Plasmid for the expression and purification of GST-TEV-EGFP-TBK1 in Spodoptera frugiperda cells (Sf9). Internal reference: SMC1631

    Depositor

    Sascha Martens

    Article

    Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

    Insert

    TBK1 (TBK1 Human)
    

    Use

    Tags

    GST and TEV-mEGFP-GG Linker

    Expression

    Bacterial and Insect

    Mutation

    Promoter

    Available since

    Oct. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pFastBac1-OSF-TRIM5alpha(chimpanzee)

    Plasmid

    #79032

    Purpose

    Baculoviral entry vector to produce Bac-to-bac baculoviruses

    Depositor

    Wesley Sundquist

    Article

    Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

    Insert

    OSF-TRIM5alpha(chimpanzee)
    

    Use

    Tags

    OneSTrEP-FLAG

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin

    Available since

    Jan. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pFastBac SpyTag-β2AR-SpyTag

    Plasmid

    #135620

    Purpose

    Expression of SpyTag-B2AR in insect cell

    Depositor

    Hee-Jung Choi

    Article

    Choi et al Science. 2019 Nov 29;366(6469):1150-1156

    Insert

    Beta 2 adrenergic receptor (ADRB2 Human)
    

    Use

    Tags

    HA-FLAG tag, Spytag, and eGFP-10xHis-R1D4 tag

    Expression

    Insect

    Mutation

    Deleted 1-24 aa, 366-413 aa

    Promoter

    Available since

    June 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFastBac HTB DPP9S D808N

    Plasmid

    #169947

    Purpose

    Purification of catalytically-dead DPP9S from insect cells

    Depositor

    Hao Wu

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    DPP9 (DPP9 Human)
    

    Use

    Tags

    HIS-TEV

    Expression

    Insect

    Mutation

    D808N

    Promoter

    polyhedrin

    Available since

    May 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pFastBac-His-Flag-Timeless

    Plasmid

    #22892

    Depositor

    Aziz Sancar

    Article

    Unsal-Kacmaz et al Mol Cell Biol. 2007 Apr . 27(8):3131-42.

    Insert

    Timeless (TIMELESS Human)
    

    Use

    Tags

    Flag and His

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Jan. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

18. PIK3CA - Exon9 E545K (pFastBac)

    Plasmid

    #16640

    Depositor

    Bert Vogelstein

    Article

    Samuels et al Science. 2004 Apr 23. 304(5670):554.

    Insert

    PIK3CA E545K (PIK3CA Human)
    

    Use

    Baculoviral

    Tags

    His

    Expression

    Mammalian

    Mutation

    E545K

    Promoter

    Available since

    March 10, 2008

    Availability

    Academic Institutions and Nonprofits only

19. pFastBac-CHD7 (787-1899)

    Plasmid

    #170145

    Purpose

    Expresses residues 787-1899 of CHD7 in insect cells

    Depositor

    Robert Kingston

    Article

    Bouazoune et al Proc Natl Acad Sci U S A. 2012 Nov 20;10

    Insert

    chromodomain helicase DNA binding protein 7 (CHD7 Human)
    

    Use

    Tags

    FLAG

    Expression

    Insect

    Mutation

    N-terminal truncation of residues 1-786; C-termin…

    Promoter

    Available since

    July 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pFastBac-CHD7 (787-2138)

    Plasmid

    #170146

    Purpose

    Expresses residues 787-2138 of CHD7 in insect cells

    Depositor

    Robert Kingston

    Article

    Bouazoune et al Proc Natl Acad Sci U S A. 2012 Nov 20;10

    Insert

    chromodomain helicase DNA binding protein 7 (CHD7 Human)
    

    Use

    Tags

    FLAG

    Expression

    Insect

    Mutation

    N-terminal truncation of residues 1-786; C-termin…

    Promoter

    Available since

    July 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge