Addgene

We narrowed to 31,071 results for: uros

Showing: 281 - 300 of 31071 results

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pcDNA3.1_V5-NEUROD1

Plasmid

#215031

Purpose

Mammalian expression of human NEUROD1

Depositor

Joanna Wysocka

Article

Kim et al Cell. 2024 Feb 1;187(3):692-711.e26. doi

Insert

neuronal differentiation 1 (NEUROD1 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pePEmax-pPuro

Plasmid

#215688

Purpose

Cloning vector for Uni-Vector prime editing system

Depositor

Kou-Juey Wu

Article

Huang et al Biotechnol J. 2024 Feb;19(2):e2300353. d

Insert

PEmax

Use

Unspecified

Tags

Expression

Mutation

Promoter

EFS promoter

Available since

Dec. 16, 2024

Availability

Academic Institutions and Nonprofits only

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AAVS1_Puro_hPGK1_mScarlet-I-TOSI_Donor

Plasmid

#178087

Purpose

HDR donor plasmid to introduce the mScarlet-I mTOR signaling indicator (TOSI) cassette to AAVS1 using AAVS1 T2 sgRNA.

Depositor

Yannick Doyon

Article

Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

Insert

mScarlet-I mTOR signaling indicator (TOSI)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Human PGK1

Available since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pHR_TRE-->2xNLS_IFP_PGK_PuroR

Plasmid

#162238

Purpose

Lentiviral vector for the expression of TRE-->2xNLS_IFP_PGK_PuroR in mammalian cells

Depositor

Wendell Lim

Article

Toda et al Science. 2020 Oct 16;370(6514):327-331.

Insert

NLS IFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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pBABEpuro-CRAF

Plasmid

#51124

Purpose

mammalian expression of wt CRAF

Depositor

Matthew Meyerson

Insert

CRAF (RAF1 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 4, 2014

Availability

Academic Institutions and Nonprofits only

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pEGFP-Aurora A

Plasmid

#198402

Purpose

Expression of EGFP-tagged Aurora A in mammalian cells

Depositor

Kenji Sugimoto

Article

Sugimoto et al Cell Struct Funct. 2002 Dec;27(6):457-67

Insert

Aurora A

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

May 1, 2023

Availability

Academic Institutions and Nonprofits only

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Opie2-PuroR

Plasmid

#131613

Purpose

Expresses PuroR and dsRed.

Depositor

Omar Akbari

Article

Kandul et al Nat Commun. 2020 Apr 30;11(1):2106. doi:

Inserts

PuroR
dsRed

Use

Tags

Expression

Insect

Mutation

Promoter

Opie2 and hr5Ie1

Available since

May 7, 2020

Availability

Academic Institutions and Nonprofits only

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pLP_mod_mNeonGreen11_P2A_puroR_CRE-GFP

Plasmid

#229227

Purpose

Modular BxB1 landing pad vector containing C-term mNeonGreen11 tag, puroR marker, and a cAMP GFP reporter.

Depositor

Willow Coyote-Maestas

Article

Howard et al bioRxiv [Preprint]. 2024 Apr 18:2024.04.

Type

Empty backbone

Use

Tags

mNeonGreen11

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pBabe.puro.CDK8.flag

Plasmid

#19758

Depositor

William Hahn

Article

Firestein et al Nature. 2008 Sep 25. 455(7212):547-51.

Insert

cyclin-dependent kinase 8 (CDK8 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 11, 2008

Availability

Academic Institutions and Nonprofits only

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pMSCVpuro-mTagBFP2

Plasmid

#96935

Purpose

Retroviral plasmid containing mTagBFP2 for labeling of cells

Depositor

Lee Grimes

Article

Kleeman et al Cytometry A. 2018 Mar 13. doi: 10.1002/c

Insert

Monomeric Tag Blue Fluorescent Protein

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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Puro-iNotch1IC

Plasmid

#75338

Purpose

doxycycline inducible Notch1 intracellular domain gene over-expression

Depositor

Danwei Huangfu

Article

Zhu et al Cell Stem Cell. 2016 Apr 27. pii: S1934-

Insert

Notch1 Intracellular domain (Notch1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

TRE-tight

Available since

May 24, 2016

Availability

Academic Institutions and Nonprofits only

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LentiGuide_CjCas9-Puro

Plasmid

#202564

Purpose

hU6-BsmBI-gRNA-Puro

Depositor

Gerald Schwank

Article

Schmidheini et al Nat Chem Biol. 2023 Sep 21. doi: 10.1038

Insert

CjCas9 sgRNA cassette

Use

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

Sept. 21, 2023

Availability

Academic Institutions and Nonprofits only

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qTAG-C-V5-Puro

Plasmid

#207730

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a V5-2A-Puro cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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HP138-puro

Plasmid

#134246

Purpose

Cas9 in a transposon array under a Tet inducible promoter. For making inducible Cas9 cell lines. Puro selection.

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

Contains Cas9

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pMSCVpuro-TagRFP657

Plasmid

#96939

Purpose

Retroviral plasmid containing TagRFP657 for labeling of cells

Depositor

Lee Grimes

Article

Kleeman et al Cytometry A. 2018 Mar 13. doi: 10.1002/c

Insert

Tag Red Fluorescent Protein 657

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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pPBpuro-ErbB1

Plasmid

#197358

Purpose

Encoding human ErbB1.

Depositor

Michiyuki Matsuda

Article

Matsuda et al J Cell Sci. 2023 Jul 31:jcs.261199. doi:

Insert

EGFR (EGFR Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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CAG-NeuroD1iresGFP

Plasmid

#45025

Depositor

Connie Cepko

Article

Cherry et al J Neurosci. 2011 May 18;31(20):7365-79.

Insert

NeuroD1 (Neurod1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

May 21, 2013

Availability

Academic Institutions and Nonprofits only

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qTAG-N-Puro-V5

Plasmid

#207697

Purpose

Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-V5 cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pRRLSIN.cPPT.PGK.NeuroD2.WPRE

Plasmid

#122054

Purpose

Lentiviral expression of NeuroD2

Depositor

Stephen Tapscott

Article

Fong et al Cell Rep. 2015 Mar 31;10(12):1937-46. do

Insert

NeuroD2 (Neurod2 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pSUPER.retro.puro-shNC

Plasmid

#113535

Purpose

Control retroviral vector.

Depositor

Blerta Xhemalce

Article

Shelton et al Cell Rep. 2018 Feb 6;22(6):1374-1383. do

Insert

shNC (scrambled control shRNA)

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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