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We narrowed to 3,637 results for: golden gate

Showing: 2981 - 3000 of 3637 results

  1. pTU2-A-RFP (colE1 origin)

    Plasmid
    #74093
    Purpose
    pTU2-A backbone, with RFP negative selection and colE1 (medium-copy) origin of replication
    Depositor
    Paul Freemont
    Article
    Moore et al ACS Synth Biol. 2016 May 2.
    Insert
    Golden Gate destination vector - Level 2, 4 TU's with RFP and colE1 origin of replication
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    R0010 Const. Promoter controlling RFP
    Available since
    April 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    74093-plasmid-map-sequence-id-138340

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  3. pTU2S-b (p15A origin)

    Plasmid
    #74089
    Purpose
    Secondary module (BpiI) site in pTU2-a backbone with p15A (low-copy) origin of replication
    Depositor
    Paul Freemont
    Article
    Moore et al ACS Synth Biol. 2016 May 2.
    Insert
    Golden Gate destination vector - Level 2, 3 TU's with secondary module and p15A origin
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    None
    Available since
    April 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    74089-plasmid-map-sequence-id-138299

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  10. pShCAST(Cas12k-TniQ)-sgRNA_entry (CJT11)

    Plasmid
    #181787
    Purpose
    Expresses 3-component ShCAST containing a Cas12k-TniQ fusion. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).
    Depositor
    Benjamin Kleinstiver
    Article
    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Insert
    ShTnsB, ShTnsC, ShCas12k-ShTniQ
    Use
    Tags
    Expression
    Bacterial
    Mutation
    nAniI = K227M, F80K, L232K
    Promoter
    Lac and J23119
    Available since
    Feb. 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    181787-plasmid-map-sequence-id-354706

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  13. pSEM398 - mosTI unc-119 - NNN - operon - mScarlet - tbb-2

    Plasmid
    #182355
    Purpose
    mScarlet co-expression cloning vector for mosTI(unc-119). Transgene inserted upstream of gpd-2 operon::mScarlet::tbb-2 3'UTR. MCS or Golden Gate (BsaI: tgcc - MCS - tgag)
    Depositor
    Christian Froekjaer-Jensen
    Article
    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068
    Type
    Empty backbone
    Use
    Tags
    Expression
    Worm
    Mutation
    Promoter
    Available since
    May 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    182355-plasmid-map-sequence-id-361664

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  14. pSEM387 - mosTI unc-119 - NNN - gfp - nls - tbb-2

    Plasmid
    #182347
    Purpose
    GFP transcriptional reporter cloning vector for mosTI(unc-119). Transgene inserted upstream of gfp(2xNLS) and tbb-2 3' UTR. MCS or Golden Gate (BsaI: tgcc - MCS - aaaa)
    Depositor
    Christian Froekjaer-Jensen
    Article
    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068
    Type
    Empty backbone
    Use
    Tags
    Expression
    Worm
    Mutation
    Promoter
    Available since
    May 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    182347-plasmid-map-sequence-id-361781

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  15. pLaNGo11-1

    Plasmid
    #126053
    Purpose
    A self-assembling split-GFP based Golden Gate vector to determine protein targeting to plastids in plant cell
    Depositor
    Ralf-Bernd Klösgen
    Article
    Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs
    Inserts
    chloroplastic FNR transit peptide
    ccdB cassette (BsaI)
    GFP11
    Use
    Binary
    Tags
    GFP1-10
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Sept. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    126053-plasmid-map-sequence-id-255676

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  16. pTU2S-b (colE1 origin)

    Plasmid
    #74092
    Purpose
    Secondary module (BpiI) site in pTU2-a backbone with colE1 (medium-copy) origin of replication
    Depositor
    Paul Freemont
    Article
    Moore et al ACS Synth Biol. 2016 May 2.
    Insert
    Golden Gate destination vector - Level 2, 3 TU's with secondary module and colE1 origin
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    None
    Available since
    April 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    74092-plasmid-map-sequence-id-141928

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  17. pICSL80036

    Plasmid
    #68259
    Purpose
    Level 0 Golden Gate Part: Coding sequence, hygromycin phophotransferase II (Escherichia coli)
    Depositor
    Nicola Patron
    Article
    Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:
    Insert
    Coding sequence, hygromycin phophotransferase II (Escherichia coli)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 20, 2015
    Availability
    Academic Institutions and Nonprofits only
    68259-plasmid-map-sequence-id-119204

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Showing: 2981 - 3000 of 3637 results