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High-Throughput Allelic Replacement Screening in Bacillus subtilis.
Diebold-Durand ML, Burmann F, Gruber S
Methods Mol Biol. 2019;2004:49-61. doi: 10.1007/978-1-4939-9520-2_5.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
117116pET-Gate2 ccdBDestination vector
117117pET-Gate2 mazEFDestination vector with mazEF toxin antitoxin system
117119pJet1.2 cat cassetteChloramphenicol resistance cassette
117120pJet1.2 specR cassetteSpectinomycin resistance cassette
117121pJet1.2 ermB cassetteErythromycin resistance cassette
117122pJet1.2 kanR cassetteKanamycin resistance cassette
117123pJet1.2 sfGFPC-terminal GFP tag
117124pJet1.2 Tev-Halo tagC-terminal Tev Halo-Tag
117126pJet1.2 HA6-TagC-terminal HA-tag
117127pJet1.2 Pk6-tagC-terminal Pk6-tag
117128pJet1.2 Tev-Avi-tagC-terminal Tev-Avi tag Tag
117129pJet1.2 'rncSsmc gene upstream homology region
117130pJet1.2 ftsYFtsY gene cassette
117131pJet1.2 sivCsmc downstream homology region

Antibodies from Article