Purpose(Empty Backbone) Destination vector with mazEF toxin antitoxin system
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||117117||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size (bp) 6400
Vector typeBackbone for Golden gate assembly for gene targeting in Bacillus subtilis
Growth in Bacteria
Growth Strain(s)ccdB Survival
Copy numberLow Copy
- Cloning method Restriction Enzyme
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Backbone vector for reconstitution of gene targeting constructs by Golden gate assembly. The MazEF toxin-antitoxin system couter-selects for single cross-over events that leads to incorporation of the vector backbone into the genome. Overhangs generated upon BsaI cleavage : CGAG (RU) and CCAT (RD).
(Gruber lab reference pSG1525)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pET-Gate2 mazEF was a gift from Stephan Gruber (Addgene plasmid # 117117 ; http://n2t.net/addgene:117117 ; RRID:Addgene_117117)
For your References section:High-Throughput Allelic Replacement Screening in Bacillus subtilis. Diebold-Durand ML, Burmann F, Gruber S. Methods Mol Biol. 2019;2004:49-61. doi: 10.1007/978-1-4939-9520-2_5. 10.1007/978-1-4939-9520-2_5 PubMed 31147909