We narrowed to 4,283 results for: biorxiv

1. WPRE (MTK 4b) (pAN2410)

   Plasmid

   #194235

   Purpose

   Mammalian Toolkit Part 4b encoding the WPRE for use with lentiviral backbones

   Depositor

   Hana El-Samad

   Article

   Weinberg et al bioRxiv 2021.04.20.440678

   Insert

   WPRE terminator
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   None

   Available since

   Sept. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBK2043-AAV-2xSp1-2xNFkB-EFSNC-dSaCas9

   Plasmid

   #223163

   Purpose

   2nd gen. AAV backbone for dSaCas9 (endonuclease dead Cas9 from Staphylococcus aureus) and Sa-gRNA Scaffold

   Depositor

   Boris Kantor

   Article

   Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

   Type

   Empty backbone

   Use

   AAV and CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Encodes aa 204-310 (MECP2)

   Promoter

   Available since

   Aug. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. HA-MBP-TEVpCS-ADGRC1 CTFdeltaTA-FLAG

   Plasmid

   #217717

   Purpose

   Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

   Depositor

   Stephen Blacklow

   Article

   Dates et al bioRxiv 2022.07.14.500097

   Insert

   CELSR1 (CELSR1 Human)
   

   Use

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   Deletion of residues 1-2453

   Promoter

   Available since

   Aug. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. FKBP-LBR(1-245)-GFP

   Plasmid

   #222425

   Purpose

   To express a truncated LBR (encoding amino acids 1-245) with N-terminal FKBP tag accessible to the cytoplasm and C-terminal GFP in mammalian cells

   Depositor

   Stephen Royle

   Article

   Downie et al bioRxiv 2024.05.31.596797

   Insert

   LBR(1-245aa) (LBR Human)
   

   Use

   Tags

   FKBP and GFP

   Expression

   Mammalian

   Mutation

   Truncation (Includes aa 1-245)

   Promoter

   Available since

   July 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pPB-EF1A-Puro-mCherry-RAB7-S72A

   Plasmid

   #221556

   Purpose

   Expresses Cherry-tagged human Rab7 with S72A mutation in mammalian cells. Compatible with piggybac transposase for genome integration.

   Depositor

   Shawn Ferguson

   Article

   Talaia et al bioRxiv [Preprint]. 2023 Dec 17:2023.12.

   Insert

   Rab7A (RAB7A Human)
   

   Use

   Piggybac

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Change serine 72 to alanine

   Promoter

   EF1a

   Available since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pSpCas9-2A-Puro-RAB7A-gRNA2 (PX459)

   Plasmid

   #221552

   Purpose

   Expresses Cas9 and gRNA for disruption of Rab7A gene in human cells

   Depositor

   Shawn Ferguson

   Article

   Talaia et al bioRxiv [Preprint]. 2023 Dec 17:2023.12.

   Insert

   gRNA targeting human Rab7A (RAB7A Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAAV-CMV-dSa VP64 Hbb

   Plasmid

   #99693

   Purpose

   Expresses dSa Cas9 fused to gold standard VP64 activator domain and Sa sgRNA for Hbb promoter, vector allows for activation of mouse Hbb, can be packaged and delivered as AAV

   Depositor

   George Church

   Article

   Vora et al bioRxiv 2018. 298620

   Insert

   dCas9 and gRNA targeting Hbb (gRNA: GGGGTAAGGGGAGCAAGGTC) (Hbb Mouse, Synthetic)
   

   Use

   AAV and CRISPR

   Tags

   VP64

   Expression

   Mutation

   dead Cas9

   Promoter

   Available since

   May 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pITESceIcmd

   Plasmid

   #218288

   Purpose

   Introduction of a cyanamide-inducible I-SceI expression cassette, use in combination with pITEdv1

   Depositor

   Claudia Vickers

   Article

   McDonnell et al bioRxiv 2024.03.26.586389

   Insert

   HO(-253, -1)-tSynth3

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pC3.1_CAG_oROS-Gr_LF(C199S)

   Plasmid

   #216114

   Purpose

   Expresses the loss-of-function version of the genetically encoded, ratiometric hydrogen peroxide sensor oROS-Gr in mamalian cells.

   Depositor

   Andre Berndt

   Article

   Lee et al bioRxiv 2024.01.31.578117

   Insert

   oROS-Gr_LF(C199S)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   April 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGEX-4T1-GST-TEV-NAP1

    Plasmid

    #217610

    Purpose

    For expression in E. coli of NAP1

    Depositor

    Sascha Martens

    Article

    Adriaenssens et al bioRxiv September 25, 202

    Insert

    NAP1 (AZI2 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    TAC promoter

    Available since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pGEX-4T1-GST-TEV-GFP-NAP1

    Plasmid

    #208870

    Purpose

    For expression in E. coli of GFP-NAP1

    Depositor

    Sascha Martens

    Article

    Adriaenssens et al bioRxiv September 25, 202

    Insert

    NAP1 (AZI2 Human)
    

    Use

    Tags

    GFP and GST-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. HA-MBP-TEVpCS-ADGRV1 CTFdeltaTA-FLAG

    Plasmid

    #217743

    Purpose

    Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

    Depositor

    Stephen Blacklow

    Article

    Dates et al bioRxiv 2022.07.14.500097

    Insert

    ADGRV1 (ADGRV1 Human)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Deletion of residues 1-5896

    Promoter

    Available since

    April 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. HA-MBP-TEVpCS-ADGRC3 CTF-FLAG

    Plasmid

    #217686

    Purpose

    Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

    Depositor

    Stephen Blacklow

    Article

    Dates et al bioRxiv 2022.07.14.500097

    Insert

    CELSR3 (CELSR3 Human)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Deletion of residues 1-2516

    Promoter

    Available since

    April 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. HA-MBP-TEVpCS-ADGRC2 CTF-FLAG

    Plasmid

    #217685

    Purpose

    Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

    Depositor

    Stephen Blacklow

    Article

    Dates et al bioRxiv 2022.07.14.500097

    Insert

    CELSR2 (CELSR2 Human)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Deletion of residues 1-2356

    Promoter

    Available since

    April 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. HA-MBP-TEVpCS-ADGRC1 CTF-FLAG

    Plasmid

    #217684

    Purpose

    Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

    Depositor

    Stephen Blacklow

    Article

    Dates et al bioRxiv 2022.07.14.500097

    Insert

    CELSR1 (CELSR1 Human)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Deletion of residues 1-2447

    Promoter

    Available since

    April 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. HA-MBP-TEVpCS-ADGRC3 CTFdeltaTA-FLAG

    Plasmid

    #217719

    Purpose

    Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

    Depositor

    Stephen Blacklow

    Article

    Dates et al bioRxiv 2022.07.14.500097

    Insert

    CELSR3 (CELSR3 Human)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Deletion of residues 1-2522

    Promoter

    Available since

    April 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. HA-MBP-TEVpCS-ADGRV1 CTF-FLAG

    Plasmid

    #217710

    Purpose

    Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

    Depositor

    Stephen Blacklow

    Article

    Dates et al bioRxiv 2022.07.14.500097

    Insert

    ADGRV1 (ADGRV1 Human)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Deletion of residues 1-5890

    Promoter

    Available since

    April 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. HA-MBP-TEVpCS-ADGRC2 CTFdeltaTA-FLAG

    Plasmid

    #217718

    Purpose

    Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

    Depositor

    Stephen Blacklow

    Article

    Dates et al bioRxiv 2022.07.14.500097

    Insert

    CELSR2 (CELSR2 Human)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Deletion of residues 1-2362

    Promoter

    Available since

    April 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. HA-MBP-TEVpCS-ADGRG2 CTF-FLAG

    Plasmid

    #217700

    Purpose

    Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

    Depositor

    Stephen Blacklow

    Article

    Dates et al bioRxiv 2022.07.14.500097

    Insert

    ADGRG2 (ADGRG2 Human)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Deletion of residues 1-606

    Promoter

    Available since

    April 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. HA-MBP-TEVpCS-ADGRG2 CTFdeltaTA-FLAG

    Plasmid

    #217733

    Purpose

    Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

    Depositor

    Stephen Blacklow

    Article

    Dates et al bioRxiv 2022.07.14.500097

    Insert

    ADGRG2 (ADGRG2 Human)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Deletion of residues 1-612

    Promoter

    Available since

    April 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge