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pEF005-sgRNA-shuffle-in
Plasmid#104441PurposeProvide and shuffle a cassette of AtU6:sgRNA-transRNA into SM-destination vectors (pRW006 and pRW004) with golden gate cloning strategy. Work together with pEF004.DepositorInsertsgRNA-transRNA transcription by U6 promoter
UseCRISPRTagsExpressionBacterial and PlantMutationPromoterAvailable sinceJan. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEF004-sgRNA-shuffle-in
Plasmid#104440PurposeProvide and shuffle a cassette of AtU6:sgRNA-transRNA into SM-destination vectors (pRW006 and pRW004) with golden gate cloning strategy. Work together with pEF005DepositorInsertsgRNA-transRNA transcription by U6 promoter
UseCRISPRTagsExpressionBacterial and PlantMutationPromoterU6 promoterAvailable sinceDec. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCDNAIIIB rhoA QL
Plasmid#61632Purposemammalian expression of rhoA constitutively active mutantDepositorInsertrhoA (RHOA Human)
UseTagsExpressionMammalianMutationQ63L, constitutively activePromoterCMVAvailable sinceJan. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
1 kb Axin2 basic
Plasmid#25701DepositorInsertAxin 2 promoter (AXIN2 Human)
UseLuciferaseTagsLuciferaseExpressionMammalianMutationPromoterAvailable sinceJune 24, 2010AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-Flex-PAK1-AID-ires-EGFP
Plasmid#83489Purposecre-dependent PAK1-AID under EF1a promoterDepositorInsertPAK1-AID-ires-EGFP
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterAvailable sinceOct. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBeast-pT7-soxA
Plasmid#190057PurposeExpress the bacterial monomeric sarcosine oxidase enzyme under the control of the strong constitutif phage promoter T7DepositorInsertSoxA
UseSynthetic Biology; Cell-free protein synthesisTagsExpressionMutationPromoterAvailable sinceNov. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
CABE-T1.17
Plasmid#193260PurposeExpression of CABE-T1.17 base editor in mammalian cells, CMV promoterDepositorInsertCABE-T1.17
UseTagsExpressionMammalianMutationD10A (Cas9), TadA mutations withinPromoterCMVAvailable sinceJan. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV-REPORT (PGK) 5xGRE-NanoLuc
Plasmid#207170PurposeGAl4 response element driven NanoLuc Lentiviral reporterDepositorInsertsNanoLuciferase
d2eGFP
5x GRE
UseLentiviral, Luciferase, and Synthetic BiologyTags3x SV40 NLS and PESTExpressionMutationPromoterGal4 response element minP and PGKAvailable sinceApril 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV5-ratERK2-Q103A
Plasmid#40816DepositorInsertMAPK1 (Mapk1 Rat)
UseTagsHis6ExpressionMammalianMutationQ103APromoterCMVAvailable sinceOct. 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
pFRT/TO/HIS/FLAG/HA-PES1
Plasmid#38085DepositorInsertPES1 pescadillo ribosomal biogenesis factor 1 (PES1 Human)
UseTagsHIS/FLAG/HAExpressionMammalianMutationPromoterAvailable sinceSept. 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
p4xSMAD_RE
Plasmid#118051PurposeGBPart - Operator/DNA Response element - 4 copies the SMAD DNA binding motifDepositorInsert4 copies the SMAD DNA binding motif
UseSynthetic Biology; Domestication of dna parts for…TagsExpressionMammalianMutationPromoterAvailable sinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pRSFDuet-1_VNp6-StefinA
Plasmid#182404PurposeBacterial expression of Vesicle Nucleating peptide6-stefinA fusionDepositorInsertVNp6-stefin (CSTA Human)
UseTagsVesicle Nucleating peptide 6 (VNp6)ExpressionBacterialMutationPromoterT7Available sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
HA-FOXO3a TM-ER-delta DB
Plasmid#8354DepositorInsertFOXO3a (FOXO3 Human)
UseTagsHAExpressionMammalianMutationT32A, S253A, S315A, ER (G525R), delta DB (deletio…PromoterAvailable sinceApril 20, 2006AvailabilityAcademic Institutions and Nonprofits only -
pBeast-pAhpC-RBS- LacZ
Plasmid#190060PurposeLacZ containing colorimetric reporter plasmid expressing it under the control of the OxyR/H2O2 inducible pAhpC promoterDepositorInsertlacZ
UseSynthetic Biology; Cell-free protein synthesisTagsExpressionMutationPromoterAvailable sinceNov. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV5-rat ERK2-K52R
Plasmid#40813DepositorInsertMAPK1 (Mapk1 Rat)
UseTagsHis6ExpressionMammalianMutationK52RPromoterCMVAvailable sinceOct. 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
Lck-mCherry-RGS
Plasmid#112935PurposePlasmid encodes a membrane located inhibitor of Ga12/13 signaling (RGS domain from p115)DepositorInsertp115RhoGEF-RGS domain
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceSept. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRK5 CASTOR2-Flag Cterm
Plasmid#84490Purposeoverexpression of CASTOR2 with Flag tagDepositorInsertCASTOR2 (GATSL2 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceOct. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUC CBA-SpCas9D10Anickase.EF1a-BFP.sgLMNApair1
Plasmid#98972PurposeCas9 Homologous Recombination Reporter. SpCas9D10A nickase and a pair of sgRNA targeting hLMNA. Generates breaks with 44bp 5' overhangs. TagBFP.DepositorInsertLMNA sgRNAs pair 1 and Cas9(D10A)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 29, 2017AvailabilityAcademic Institutions and Nonprofits only
