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pWZL Neo Myr Flag CAMK1G

Plasmid

#20438

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

CAMK1G (CAMK1G Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
YC 3.6 Cameleon

Plasmid

#58182

Purpose

Localization: Cytosolic Ca Biosensor, Excitation: 433 / 515, Emission: 475 / 528

Depositor

Michael Davidson

Type

Empty backbone

Tags

ECFP and Venus

Expression

Mammalian

Available Since

April 28, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pcDNA3.1 6B codon-optimized NLRP1 CARD-mCherry WT

Plasmid

#163991

Purpose

Mammalian expression vector encoding codon-optimized human NLRP1 CARD with a C-terminal mCherry tag for imaging

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

NLRP1 CARD (NLRP1 Human)

Tags

mCherry

Expression

Mammalian

Available Since

Feb. 12, 2021

Availability

Academic Institutions and Nonprofits only

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EPHA3_HUMAN_D0

Plasmid

#79708

Purpose

This plasmid encodes the kinase domain of EPHA3. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

EPHA3 (EPHA3 Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
SRC_HUMAN_D0

Plasmid

#79700

Purpose

This plasmid encodes the kinase domain of SRC. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

SRC (SRC Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
DYR1A_HUMAN_D0

Plasmid

#79690

Purpose

This plasmid encodes the kinase domain of DYR1A. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

DYRK1A (DYRK1A Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA3.1 NLRP1-delPYD-delCARD-TEV-GFP-FLAG codon-optimized

Plasmid

#166834

Purpose

Expression and purification of NLRP1 (without the PYD and CARD) from mammalian cells

Depositor

Hao Wu

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

NLRP1 (NLRP1 Human)

Tags

TEV-GFP-FLAG

Expression

Mammalian

Promoter

CMV

Available Since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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pET His6 MBP TEV HDAC8_374

Plasmid

#122174

Purpose

Expresses a truncated construct of histone deacetylase 8 for crystallography bearing an N-terminal tobacco etch virus protease-cleavable hexahistidine-maltose binding protein tag

Depositor

David Christianson

Article

Porter et al ACS Chem Biol. 2017 Sep 15;12(9):2281-22

Insert

His6 MBP TEV HDAC8_374 (HDAC8 Human)

Tags

hexahistidine tag and tobacco etch virus protease…

Expression

Bacterial

Mutation

Residues Met1–Pro7 and H375-V377 have been delete…

Promoter

T7

Available Since

March 5, 2019

Availability

Academic Institutions and Nonprofits only

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ABL1_HUMAN_D0

Plasmid

#79727

Purpose

This plasmid encodes the kinase domain of ABL1. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

ABL1 (ABL1 Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA3.1 6A NLRP1 CARD-FLAG WT

Plasmid

#163978

Purpose

Mammalian expression vector encoding human NLRP1 CARD with a C-terminal FLAG tag

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

NLRP1 CARD (NLRP1 Human)

Tags

FLAG

Expression

Mammalian

Available Since

Jan. 24, 2021

Availability

Academic Institutions and Nonprofits only

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AURKA_HUMAN_D0

Plasmid

#79739

Purpose

This plasmid encodes the kinase domain of AURKA. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

AURKA (AURKA Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
OA-1136J

Plasmid

#153023

Purpose

Express His-MBP-TEV-CasRx recombinant protein

Depositor

Omar Akbari

Article

Brogan et al ACS Sens. 2021 Nov 26;6(11):3957-3966. d

Insert

CasRx

Tags

His-MBP-TEV

Expression

Bacterial

Promoter

T7

Available Since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 6A NLRP1 UPA-CARD-FLAG WT

Plasmid

#163979

Purpose

Mammalian expression vector encoding human NLRP1 UPA-CARD with a C-terminal FLAG tag

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

NLRP1 UPA-CARD (NLRP1 Human)

Tags

FLAG

Expression

Mammalian

Available Since

Jan. 24, 2021

Availability

Academic Institutions and Nonprofits only

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NEK1_HUMAN_D0

Plasmid

#79725

Purpose

This plasmid encodes the kinase domain of NEK1. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

NEK1 (NEK1 Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
MERTK_HUMAN_D0

Plasmid

#79705

Purpose

This plasmid encodes the kinase domain of MERTK. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

MERTK (MERTK Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA3.1 6A NLRP1 UPA-CARD-FLAG E1461R

Plasmid

#163990

Purpose

Mammalian expression vector encoding human NLRP1 UPA-CARD (dimer interface mutant) with a C-terminal FLAG tag

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

NLRP1 UPA-CARD (NLRP1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

E1461R

Available Since

Feb. 12, 2021

Availability

Academic Institutions and Nonprofits only

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MARK3_HUMAN_D0

Plasmid

#79740

Purpose

This plasmid encodes the kinase domain of MARK3. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

MARK3 (MARK3 Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA3.1 6A NLRP1 UPA-CARD-FLAG M1457A

Plasmid

#163988

Purpose

Mammalian expression vector encoding human NLRP1 UPA-CARD (dimer interface mutant) with a C-terminal FLAG tag

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

NLRP1 UPA-CARD (NLRP1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

M1457A

Available Since

May 5, 2021

Availability

Academic Institutions and Nonprofits only

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EPHB1_HUMAN_D0

Plasmid

#79694

Purpose

This plasmid encodes the kinase domain of EPHB1. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

EPHB1 (EPHB1 Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
STK3_HUMAN_D0

Plasmid

#79689

Purpose

This plasmid encodes the kinase domain of STK3. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

STK3 (STK3 Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only