We narrowed to 4,714 results for: Drosophila

1. P[acman]-Alpha2

   Plasmid

   #165859

   Purpose

   Alpha2 Basic cloning vector GB2.0 compatible P[acman]-derived backbone. Allows for copy induction from low to high using AutoFOS or similar. Requires EPI300 bacterial strain (DH10B Derived) or similar. uses blue-white screening

   Depositor

   Koen Venken

   Article

   Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCFJ909

   Plasmid

   #44480

   Depositor

   Erik Jorgensen

   Article

   Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

   Insert

   Minimal Mos1, unc-119, MCS
   

   Use

   Multiple cloning site vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 30, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBS-KS-attB1-2-PT-SA-SD-0-2xTY1-V5

   Plasmid

   #61255

   Purpose

   CRISPR-RMCE, second step attB plasmid, intron phase 0, exchange dsRed with 2xTY1 and V5 tags cassette

   Depositor

   Frank Schnorrer

   Article

   Zhang et al G3 (Bethesda). 2014 Oct 15. pii: g3.114.

   Insert

   2xTY1-V5
   

   Use

   CRISPR

   Tags

   TY1 and V5

   Expression

   Mutation

   Promoter

   Available since

   Nov. 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pT7-EGFP-C1-HsNXF-1_K

   Plasmid

   #146740

   Purpose

   Mammalian Expression of HsNxf-1

   Depositor

   Elisa Izaurralde

   Article

   Gatfield et al EMBO J. 2003 Aug 1. 22(15):3960-70.

   Insert

   HsNxf-1 (NXF1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   S144G relative to NM_006362.4

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCRISPaint-T2A-sfGFP-3xP3-RFP

   Plasmid

   #127557

   Purpose

   CRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-sfGFP-SV40 and 3xP3-RFP visible eye marker.

   Depositor

   Norbert Perrimon

   Article

   Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

   Insert

   sfGFP
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   July 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDEST-HemmarR2

   Plasmid

   #112813

   Purpose

   gateway cloning destination vector for expressing enhancer driven CD4-tdTomato

   Depositor

   Chun Han

   Article

   Poe et al Genetics. 2018 Nov 30. pii: genetics.118

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCRISPaint-T2A-FLPo-3xP3-RFP

   Plasmid

   #127561

   Purpose

   CRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-FLPo-SV40 and 3xP3-RFP visible eye marker.

   Depositor

   Norbert Perrimon

   Article

   Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

   Insert

   FLPo
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Oct. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDEST-HemmarG2

   Plasmid

   #112814

   Purpose

   gateway cloning destination vector for expressing enhancer driven CD4-tdGFP

   Depositor

   Chun Han

   Article

   Poe et al Genetics. 2018 Nov 30. pii: genetics.118

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCRISPaint-T2A-Gal80-3xP3-RFP

   Plasmid

   #127562

   Purpose

   CRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-Gal80-SV40 and 3xP3-RFP visible eye marker.

   Depositor

   Norbert Perrimon

   Article

   Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

   Insert

   Gal80
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Oct. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAC-CR7T-gRNA2.1-nlsBFP

    Plasmid

    #170515

    Purpose

    gRNA cloning vector containing a CR7T promoter, gRNA(2.1) scaffold, and a Ubi-mTagBFP-NLS marker; optimized for germline CRISPR.

    Depositor

    Chun Han

    Article

    Koreman et al Proc Natl Acad Sci U S A. 2021 Apr 6;118

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    CR7T

    Available since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMH3

    Plasmid

    #52528

    Purpose

    serves as PCR-template to generate HR donors for C-terminal GFP-tag

    Depositor

    Klaus Foerstemann

    Article

    Bottcher et al Nucleic Acids Res. 2014 Apr 19.

    Insert

    eGFP and Blasticigin resistance cassette
    

    Use

    Blunt cloning kit

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pZG61

    Plasmid

    #202575

    Purpose

    RNA editing in plants. Plasmid contains 35S-dADARcd-LIC1-ccdB-LIC2-35S-hptII

    Depositor

    Guoyong Xu

    Article

    Zhou et al Cell Discov. 2021 Aug 24;7(1):72. doi: 1

    Insert

    HyperADARcd
    

    Use

    Tags

    Expression

    Plant

    Mutation

    E488Q

    Promoter

    Available since

    Dec. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJFRC-20X-UAS-ADARcd

    Plasmid

    #81173

    Purpose

    identification of cell-specific targets of RNA binding proteins

    Depositor

    Michael Rosbash

    Article

    McMahon et al Cell. 2016 Mar 30. pii: S0092-8674(16)30

    Insert

    UAS-ADARcd
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    UAS

    Available since

    Aug. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBlastR-12xLexAOp-mCherry-MiniWhite

    Plasmid

    #165917

    Purpose

    Blasticidin resistant 12xLexAOp mCherry Mini-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screening

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    Selectable LexAOp Response Vector
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCRISPaint-T2A-Gal4DBD-3xP3-RFP

    Plasmid

    #127564

    Purpose

    CRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-Gal4DBD-SV40 and 3xP3-RFP visible eye marker.

    Depositor

    Norbert Perrimon

    Article

    Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

    Insert

    Gal4DBD
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    July 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCRISPaint-T2A-p65-3xP3-RFP

    Plasmid

    #127565

    Purpose

    CRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-p65-SV40 and 3xP3-RFP visible eye marker.

    Depositor

    Norbert Perrimon

    Article

    Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

    Insert

    p65
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBFv-UAS3-GFP1-10

    Plasmid

    #138398

    Purpose

    Expresses split GFP fragment (GFP1-10)

    Depositor

    Shu Kondo

    Article

    Kondo et al Cell Rep. 2020 Jan 7;30(1):284-297.e5. d

    Insert

    GFP1-10
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCRISPaint-T2A-QF2-3xP3-RFP

    Plasmid

    #127559

    Purpose

    CRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-QF2-SV40 and 3xP3-RFP visible eye marker.

    Depositor

    Norbert Perrimon

    Article

    Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

    Insert

    QF2
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. p-attB-min.hsp70P-FRT-STOP#1-FRT-DamMyc[open]

    Plasmid

    #71809

    Purpose

    Generating transgenic flies for FLP-inducible DamID

    Depositor

    Bas van Steensel

    Article

    Pindyurin et al Nucleic Acids Res. 2016 Jul 8;44(12):564

    Insert

    min.hsp70P-FRT-STOP#1-FRT-Dam-Myc[open]
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pUb dRxCas13d + RxCas13d guide RNA

    Plasmid

    #176304

    Purpose

    Expresses catalytic dead RxCas13d and its associated guide RNA in Drosophila cells

    Depositor

    Jeremy Wilusz

    Article

    Ai et al Nucleic Acids Res. 2022 Mar 4. pii: 6542

    Insert

    RxCas13d
    

    Use

    Tags

    HA tag

    Expression

    Insect

    Mutation

    R239A, H244A, R858A, H863A

    Promoter

    Ubi-p63e

    Available since

    March 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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