We narrowed to 6,239 results for: nls

1. pHEN-CoV2-nanobody-F1

   Plasmid

   #194592

   Purpose

   Bacterial expression of F1 nanobody targeting the receptor binding domain (RBD) of SARS-CoV-2 spike

   Depositor

   Gerald McInerney

   Article

   Hanke et al Sci Adv. 2022 Mar 25;8(12):eabm0220. doi

   Insert

   F1 (S )
   

   Expression

   Bacterial

   Available Since

   Feb. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHEN-CoV2-nanobody-E2

   Plasmid

   #194590

   Purpose

   Bacterial expression of E2 nanobody targeting the receptor binding domain (RBD) of SARS-CoV-2 spike

   Depositor

   Gerald McInerney

   Article

   Hanke et al Sci Adv. 2022 Mar 25;8(12):eabm0220. doi

   Insert

   E2 (S )
   

   Expression

   Bacterial

   Available Since

   Feb. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET42b-BE3

   Plasmid

   #87437

   Purpose

   Expresses BE3-NLS with an N-terminal His Tag (His6) for bacterial expression

   Depositor

   David Liu

   Article

   Rees et al Nat Commun. 2017 Jun 6;8:15790. doi: 10.

   Insert

   BE3 (Apobec1 Rat, Bacillus phage PBS2, Human)
   

   Tags

   His6

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHEN-CoV2-nanobody-C7

   Plasmid

   #194591

   Purpose

   Bacterial expression of C7 nanobody targeting the receptor binding domain (RBD) of SARS-CoV-2 spike

   Depositor

   Gerald McInerney

   Article

   Hanke et al Sci Adv. 2022 Mar 25;8(12):eabm0220. doi

   Insert

   C7 (S )
   

   Expression

   Bacterial

   Available Since

   Feb. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA Flag-RARalpha

   Plasmid

   #35555

   Depositor

   Harish Srinivas, Jonathan Kurie

   Article

   Srinivas et al Mol Cell Biol. 2005 Feb;25(3):1054-69.

   Insert

   RARalpha (RARA Human)
   

   Tags

   Flag and His

   Expression

   Mammalian

   Available Since

   April 5, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. SUV[SET]-dCas9

   Plasmid

   #100088

   Purpose

   Catalytic domain [SET] of human SUVAR39H1 fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression

   Depositor

   David Segal

   Article

   O'Geen et al Nucleic Acids Res. 2017 Sep 29;45(17):99

   Insert

   SUVAR39H1 (SUV39H1 Human)
   

   Use

   CRISPR

   Tags

   3XFLag-NLS-SUV[SET]-dCas9-NLS

   Expression

   Mammalian

   Mutation

   deleted aa 1-76

   Promoter

   CMV

   Available Since

   Oct. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET42b-ABE7.10

   Plasmid

   #120398

   Purpose

   Expresses ABE7.10-NLS with an N-terminal His Tag (His6) for bacterial expression

   Depositor

   Junjiu Huang

   Article

   Liang et al Nat Commun. 2019 Jan 8;10(1):67. doi: 10

   Insert

   ABE7.10
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   Mammalian codon-optimized

   Promoter

   T7

   Available Since

   April 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDONR221 MLK1

   Plasmid

   #60540

   Purpose

   Donor vector for human MLK1

   Depositor

   John Brognard

   Article

   Fawdar et al Proc Natl Acad Sci U S A. 2013 Jul 23;11

   Insert

   MLK1 (MAP3K9 Human)
   

   Use

   Gateway donor vector

   Available Since

   Feb. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pYTRW26K_1Ti1

   Plasmid

   #177292

   Purpose

   genomic integration of genes (inserted in I-SceI-site) via rrn-interposon/SacB with TcR and eYFP

   Depositor

   Karl-Erich Jaeger

   Article

   Weihmann et al FEMS Microbes. 2022 Dec 19;4:xtac030. do

   Insert

   PsacB-sacB
   

   Use

   Synthetic Biology; Yeast expression, rrn genomic …

   Expression

   Bacterial

   Available Since

   Aug. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. MBP-hnRNPA2_FL_WT

    Plasmid

    #98662

    Purpose

    Expresses MBP-tagged full length hnRNPA2

    Depositor

    Nicolas Fawzi

    Article

    Ryan et al Mol Cell. 2018 Jan 17. pii: S1097-2765(1

    Insert

    hnRNPA2 (HNRNPA2B1 Human)
    

    Tags

    6xHis-MBP

    Expression

    Bacterial

    Available Since

    Jan. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pInt-ERCreER

    Plasmid

    #190040

    Purpose

    This is an integration donor plasmid to insert a DNA fragment for doxycycline inducible ERT2-Cre-ERT2 at the AAVS1 locus. It works with an AAVS1 targeting CRISPR/Cas9 construct (Addgene #72833).

    Depositor

    Kayoko Tanaka

    Article

    Hindul et al Biol Open. 2022 Feb 15;11(2). pii: 27408

    Insert

    ERT2-Cre-ERT2 flanked by AAVS1
    

    Use

    CRISPR and TALEN

    Expression

    Mammalian

    Available Since

    Jan. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. SpCas9-NG-APOBEC-nCas9-Ung

    Plasmid

    #198539

    Purpose

    Expresses human codon-optimized SpCas9-NG-APOBEC-nCas9-Ung and blasticidin resistance: EFS promoter-SpCas9-NG-APOBEC-nCas9-Ung-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

    Insert

    SpCas9-NG-APOBEC-nCas9-Ung
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Promoter

    EFS

    Available Since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET42b-HF-BE3

    Plasmid

    #87438

    Purpose

    Expresses HF-BE3-NLS with an N-terminal His Tag (His6) for bacterial expression

    Depositor

    David Liu

    Article

    Rees et al Nat Commun. 2017 Jun 6;8:15790. doi: 10.

    Insert

    HF-BE3 (Apobec1 Rat, Bacillus phage PBS2, Human)
    

    Tags

    His6

    Expression

    Bacterial

    Available Since

    June 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-hSYN-DRD2-V2tail-TevN-BLITz1-TetR-VP16-bGHpA

    Plasmid

    #89874

    Purpose

    expresses DRD2-iTango component in mammalian cells for AAV production

    Depositor

    Hyungbae Kwon

    Article

    Lee et al Nat Methods. 2017 May;14(5):495-503. doi

    Insert

    DRD2-V2tail-TevN-BLITz1-TetR-VP16
    

    Use

    AAV

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    short isoform of DRD2 (deletion of 242-270)

    Promoter

    Human synapsin

    Available Since

    May 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLNCX myr Akt1

    Plasmid

    #17245

    Depositor

    Joan Brugge

    Article

    Schmelzle et al Proc Natl Acad Sci U S A. 2007 Mar 6. 10

    Insert

    Akt1 (Akt1 Mouse)
    

    Use

    Retroviral

    Tags

    Myr

    Expression

    Mammalian

    Mutation

    Constitutively active

    Available Since

    Jan. 18, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCR1163

    Plasmid

    #111099

    Purpose

    Expresses FANCD2_+_10068200.23-P1P2 sgRNA in pCR1068

    Depositor

    Jacob Corn

    Article

    Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

    Insert

    FANCD2 gRNA (FANCD2 Human)
    

    Use

    Lentiviral

    Available Since

    Oct. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pACYC-T7-SpCas9-T7-EGFP_gRNA1-T7term (BPK848)

    Plasmid

    #181745

    Purpose

    dual T7 promoter vector for bacterial expression of SpCas9 with a c-terminal NLS and 3xFLAG tag, along with an SpCas9 sgRNA targeting EGFP site 1

    Depositor

    Benjamin Kleinstiver

    Article

    Christie et al Nat Biotechnol. 2022 Oct 6. pii: 10.1038

    Insert

    human/zebrafish codon optimized SpCas9
    

    Use

    In vitro transcription; t7 promoter

    Tags

    NLS(SV40)-3xFLAG

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSBbi-FoxO1_1R_10A_3D

    Plasmid

    #106278

    Purpose

    Fluorescent fusion protein for FoxO1

    Depositor

    Laura Heiser

    Article

    Gross et al Cell Syst. 2019 Dec 18;9(6):580-588.e4.

    Inserts

    Foxo1a (Foxo1 Mouse)
    NLS-mCherry-NLS-P2A-Puromycin
    

    Tags

    Clover fluorescent protein

    Expression

    Mammalian

    Mutation

    Deleted amino acids 401-636, S209A, H212R, S215A,…

    Promoter

    EF1a/RPBSA

    Available Since

    July 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. TC1316

    Plasmid

    #164883

    Purpose

    CMV-bpNLS-dCas13d-bpNLS-ADARdd-mCherry, ADARdd embeded in loop3

    Depositor

    Tian Chi

    Article

    Liu et al EMBO J. 2020 Nov 16;39(22):e104748. doi:

    Insert

    dcas13d-ADARdd
    

    Expression

    Mammalian

    Mutation

    dCas13d L560-G586 deleted

    Promoter

    CMV, T7

    Available Since

    March 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. MSP712

    Plasmid

    #65768

    Purpose

    Bacterial expression plasmid for Sp-dCas9 & sgRNA (need to clone in spacer into BsaI sites): T7-humanSpdCas9(D10A/H840A)-T7-BsaIcassette-Sp-sgRNA

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

    Insert

    mammalian codon-optimized Streptococcus pyogenes dCas9 (D10A/H840A)-NLS-3XFlag, and SpCas9 gRNA
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Bacterial

    Mutation

    D10A and H840A mutations in Cas9

    Promoter

    T7 (x2)

    Available Since

    June 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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