We narrowed to 4,097 results for: biorxiv

1. pPB-EF1A-Puro-mCherry-RAB7-S72A

   Plasmid

   #221556

   Purpose

   Expresses Cherry-tagged human Rab7 with S72A mutation in mammalian cells. Compatible with piggybac transposase for genome integration.

   Depositor

   Shawn Ferguson

   Article

   Talaia et al bioRxiv [Preprint]. 2023 Dec 17:2023.12.

   Insert

   Rab7A (RAB7A Human)
   

   Use

   Piggybac

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Change serine 72 to alanine

   Promoter

   EF1a

   Available Since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSpCas9-2A-Puro-RAB7A-gRNA2 (PX459)

   Plasmid

   #221552

   Purpose

   Expresses Cas9 and gRNA for disruption of Rab7A gene in human cells

   Depositor

   Shawn Ferguson

   Article

   Talaia et al bioRxiv [Preprint]. 2023 Dec 17:2023.12.

   Insert

   gRNA targeting human Rab7A (RAB7A Human)
   

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGP-AAV-CAG-flex-iGABASnFR2n-WPRE

   Plasmid

   #218881

   Purpose

   AAV-mediated expression of improved GABA sensor (negative change in fluorescence)

   Depositor

   GENIE Project

   Article

   Kolb et al bioRxiv 2025.03.25.644953

   Insert

   iGABASnFR2n
   

   Use

   AAV and Cre/Lox

   Expression

   Mammalian

   Mutation

   S99A F104H R168P

   Promoter

   CAG

   Available Since

   June 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGP-AAV-GFAP-iGABASnFR2(no bind)-WPRE

   Plasmid

   #218873

   Purpose

   AAV-mediated expression of improved GABA sensor control (no change in fluorescence)

   Depositor

   GENIE Project

   Article

   Kolb et al bioRxiv 2025.03.25.644953

   Has Service

   AAV1 and AAV5

   Insert

   iGABASnFR2(no bind)
   

   Use

   AAV

   Expression

   Mammalian

   Mutation

   S99A F102G R168P

   Promoter

   GFAP

   Available Since

   June 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-CMV-dSa VP64 Hbb

   Plasmid

   #99693

   Purpose

   Expresses dSa Cas9 fused to gold standard VP64 activator domain and Sa sgRNA for Hbb promoter, vector allows for activation of mouse Hbb, can be packaged and delivered as AAV

   Depositor

   George Church

   Article

   Vora et al bioRxiv 2018. 298620

   Insert

   dCas9 and gRNA targeting Hbb (gRNA: GGGGTAAGGGGAGCAAGGTC) (Hbb Synthetic, Mouse)
   

   Use

   AAV and CRISPR

   Tags

   VP64

   Mutation

   dead Cas9

   Available Since

   May 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pITESceIcmd

   Plasmid

   #218288

   Purpose

   Introduction of a cyanamide-inducible I-SceI expression cassette, use in combination with pITEdv1

   Depositor

   Claudia Vickers

   Article

   McDonnell et al bioRxiv 2024.03.26.586389

   Insert

   HO(-253, -1)-tSynth3

   Expression

   Yeast

   Available Since

   May 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGP-AAV-syn-iGABASnFR2(no bind)-WPRE

   Plasmid

   #218876

   Purpose

   AAV-mediated expression of improved GABA sensor control (no change in fluorescence)

   Depositor

   GENIE Project

   Article

   Kolb et al bioRxiv 2025.03.25.644953

   Has Service

   AAV1 and AAV5

   Insert

   iGABASnFR2(no bind)
   

   Use

   AAV

   Expression

   Mammalian

   Mutation

   S99A F102G R168P

   Promoter

   Synapsin

   Available Since

   May 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGEX-4T1-GST-TEV-NAP1

   Plasmid

   #217610

   Purpose

   For expression in E. coli of NAP1

   Depositor

   Sascha Martens

   Article

   Adriaenssens et al bioRxiv September 25, 202

   Insert

   NAP1 (AZI2 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Promoter

   TAC promoter

   Available Since

   April 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGEX-4T1-GST-TEV-GFP-NAP1

   Plasmid

   #208870

   Purpose

   For expression in E. coli of GFP-NAP1

   Depositor

   Sascha Martens

   Article

   Adriaenssens et al bioRxiv September 25, 202

   Insert

   NAP1 (AZI2 Human)
   

   Tags

   GFP and GST-TEV

   Expression

   Bacterial

   Available Since

   April 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. HA-MBP-TEVpCS-ADGRC2 CTF-FLAG

    Plasmid

    #217685

    Purpose

    Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

    Depositor

    Stephen Blacklow

    Article

    Dates et al bioRxiv 2022.07.14.500097

    Insert

    CELSR2 (CELSR2 Human)
    

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Deletion of residues 1-2356

    Available Since

    April 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. HA-MBP-TEVpCS-ADGRC1 CTF-FLAG

    Plasmid

    #217684

    Purpose

    Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

    Depositor

    Stephen Blacklow

    Article

    Dates et al bioRxiv 2022.07.14.500097

    Insert

    CELSR1 (CELSR1 Human)
    

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Deletion of residues 1-2447

    Available Since

    April 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. HA-MBP-TEVpCS-ADGRV1 CTF-FLAG

    Plasmid

    #217710

    Purpose

    Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

    Depositor

    Stephen Blacklow

    Article

    Dates et al bioRxiv 2022.07.14.500097

    Insert

    ADGRV1 (ADGRV1 Human)
    

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Deletion of residues 1-5890

    Available Since

    April 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. HA-MBP-TEVpCS-ADGRG2 CTF-FLAG

    Plasmid

    #217700

    Purpose

    Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

    Depositor

    Stephen Blacklow

    Article

    Dates et al bioRxiv 2022.07.14.500097

    Insert

    ADGRG2 (ADGRG2 Human)
    

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Deletion of residues 1-606

    Available Since

    April 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pX330_EZH2gRNA15

    Plasmid

    #199587

    Purpose

    Contains guide RNA to 5' end of mouse EZH2 gene

    Depositor

    Mauro Calabrese

    Article

    Cherney et al bioRxiv. 2023 Apr 3:2023.04.03.535391. d

    Insert

    EZH2 (Ezh2 Mouse)
    

    Use

    CRISPR and Mouse Targeting

    Expression

    Bacterial and Mammalian

    Available Since

    May 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pX330_EZH2gRNA16

    Plasmid

    #199588

    Purpose

    Contains guide RNA to 3' end of mouse EZH2 gene

    Depositor

    Mauro Calabrese

    Article

    Cherney et al bioRxiv. 2023 Apr 3:2023.04.03.535391. d

    Insert

    EZH2 (Ezh2 Mouse)
    

    Use

    CRISPR and Mouse Targeting

    Expression

    Bacterial and Mammalian

    Available Since

    May 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pX330_EZH2gRNA17

    Plasmid

    #199589

    Purpose

    Contains guide RNA to 3' end of mouse EZH2 gene

    Depositor

    Mauro Calabrese

    Article

    Cherney et al bioRxiv. 2023 Apr 3:2023.04.03.535391. d

    Insert

    EZH2 (Ezh2 Mouse)
    

    Use

    CRISPR and Mouse Targeting

    Expression

    Bacterial and Mammalian

    Available Since

    May 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. OA-1067L

    Plasmid

    #200253

    Purpose

    pBac-U6-gRNA(doublesex+Intersex+bTublin)-3xp3-tdTomato

    Depositor

    Omar Akbari

    Article

    Li et al bioRxiv. 2023 Nov 15:2023.04.18.537404.

    Insert

    6 gRNAs targeting Doublesex, Intersex, bTublin
    

    Expression

    Insect

    Available Since

    May 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pminiT-mEmerald-MED1 donor

    Plasmid

    #197867

    Purpose

    Donor plasmid used for building mEmerald-MED1 knock-in mESCs

    Depositor

    James Zhe Liu

    Article

    Dong et al bioRxiv 2022.10.26.513917

    Insert

    CRSP210 (Med1 Mouse)
    

    Use

    Mouse Targeting

    Tags

    mEmerald

    Promoter

    NA

    Available Since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTij_sg_mmu_Med6_C_terminal

    Plasmid

    #197869

    Purpose

    CRISPR/Cas9/sgRNA plasmid for cutting Med6 C terminal and building MED6-mEmerald/Halo knock-in mESCs

    Depositor

    James Zhe Liu

    Article

    Dong et al bioRxiv 2022.10.26.513917

    Insert

    Med6 (Med6 Mouse)
    

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pROS13_IDH2 #7

    Plasmid

    #166102

    Purpose

    This plasmid express a sgRNA that targets the IDH2 gene. This allows Cas9 to make a double-strand break and facilitate integration of LOV2 at position IDH2-135 by homologous recombination.

    Depositor

    Matthias Heinemann

    Article

    Chen et al bioRxiv

    Insert

    IDH2 sgRNA #7 (IDH2 Budding Yeast)
    

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    April 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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