We narrowed to 8,385 results for: Dos
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Plasmid#108883PurposeMultisite gateway vector for 5' tagging with the human histone H2B sequence (for nuclear localisation)DepositorInsertH2B (human amino acid sequence but different codon usage) (H2BC21 Synthetic, Human)
UseGateway CloningMutationhuman amino acid sequence but different codon usa…Available SinceNov. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCK015
Plasmid#105347PurposeBacterial translocation fo proteins into plant cellsDepositorInsertsecretion signal, ccdB cassette
UseBacterial secretionMutationBsaI restriction sites removedAvailable SinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJOG387
Plasmid#105338PurposeSynthetic biology; gateway destination vector assemblyDepositorTypeEmpty backboneUseSynthetic BiologyAvailable SinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSUPER.retro.puro-shMePCE_shLARP7
Plasmid#113538PurposeRetroviral vector designed to knock down MePCE and LARP7 simultaneously.DepositorInsertshMePCE and shLARP7 (MEPCE Human)
UseRetroviralAvailable SinceJuly 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDM1080
Plasmid#108980PurposeKnockout cassette NourseothricinDepositorInsertKnockout cassette Nourseothricin
UseCre/Lox; Dictyostelium discoideum knockout vectorPromotercoaAAvailable SinceJuly 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDM1020
Plasmid#108977Purposeshuttle vector, C-terminal mCherry taggingDepositorTypeEmpty backboneUseDictyostelium discoideum shuttle vectorTagsmCherryAvailable SinceJune 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDNA-hNgAgo
Plasmid#104384Purposehuman codon optimized NgAgo; pCDNA3 vector backbone, mammalian expressionDepositorInserthNgAgo
Tags3XFlag, Nucleoplasmin NLS, and SV40 NLSExpressionMammalianPromoterCMVAvailable SinceApril 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pOEM-GED-mCherry
Plasmid#102781PurposeBaculovirus rescue vector, VSVGED pseudotype, mCherryDepositorInsertmCherry
UseBaculoviral rescue shuttle vectorExpressionInsect and MammalianPromoterCMVAvailable SinceDec. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
H14-MBP-SUMO-Cys-Linker-BAF_L58R
Plasmid#101780PurposeExpresses His-MBP-tagged mutant BAF (L58R) in E.coli (SUMO-cleavable)DepositorInsertBAF (BANF1) (BANF1 Human)
TagsHis14-MBP-SUMOExpressionBacterialMutationL58R mutationPromoterT5Available SinceDec. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
H14-MBP-SUMO-FLAG-LEM
Plasmid#101778PurposeExpresses His-MBP-tagged mutant LEM-domain (FLAG-tagged) in E.coli (SUMO-cleavable)DepositorInsertMAN1 (LEMD3 Human)
TagsHis14-MBP-SUMO-FLAGExpressionBacterialMutationamino acids 6-51 of human MAN1 (LEM domain)PromoterT5Available SinceDec. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
plenti-CAG-IKZF3-Q147H-FLAG-IRES-GFP
Plasmid#69050Purposelentiviral expression of IKZF3 with Q147H mutation and FLAG tagDepositorInsertIKZF3 (IKZF3 Human)
UseLentiviralTagsFLAG and IRES-GFPExpressionMammalianMutationQ147HPromoterCAGAvailable SinceOct. 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
36INS-J23100_AB
Plasmid#78666PurposeInsulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected.Modified from BBa_J23100. [A:36INS-J23100:B]DepositorInsertInsulated J23100 Promoter
UseSynthetic BiologyAvailable SinceAug. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
MO70-hCRACR2A-isoform-c
Plasmid#79591PurposeTransient and retroviral expressionDepositorAvailable SinceJune 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
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p123shy1-3HA
Plasmid#67876Purposeencodes shy1 with a C-terminal 3xHA-tag under control of its endogenous promoter.DepositorInsertshy1
UseExpression in ustilago maydisTagsHAPromotershy1Available SinceAug. 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRS426-TUB1-internal6xHis - human TUBA1a Cterminal tail
Plasmid#60392PurposeExpression of chimeric yeast alpha tubulin (TUB1) - internal 6xHis with human alpha tubulin Cterminal tail (TUBA1a) using a GAL promoterDepositorInsertTUB1 (TUB1 Budding Yeast, Synthetic)
ExpressionYeastMutationinternal 6xHis (see supplement figure 4), amino a…PromoterGALAvailable SinceJan. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMTB-Multibow-mY
Plasmid#60988PurposePart of Multibow set of constructs--each FP gene is initially not expressed and then adopts a permanent ON or OFF status upon Cre-mediated recombination. Encodes membrane bound citrineDepositorInsertcitrine
UseZebrafishTagsmembrane tagPromoterBactin2Available SinceJan. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMTB-Multibow-mO
Plasmid#60989PurposePart of Multibow set of constructs--each FP gene is initially not expressed and then adopts a permanent ON or OFF status upon Cre-mediated recombination. Encodes membrane bound mKODepositorInsertmKO
UseZebrafishTagsmembrane tagPromoterBactin2Available SinceJan. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-GFP (F64L, S65T, V163A)-TRP1
Plasmid#53184Purposechromosomal tagging with stable GFPDepositorAvailable SinceMay 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV-myc-CD4-Sema4D
Plasmid#51603Purposeexpresses Sema4D/CD4 chimera with intracellular Sema4D, extracellular CD4DepositorInsertCD4-Sema4D
TagsmycExpressionMammalianMutationSema4D amino acids 1-754 have been replaced with …PromoterCMVAvailable SinceApril 7, 2014AvailabilityAcademic Institutions and Nonprofits only