We narrowed to 10,039 results for: pET

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pET27b-ecDHFR-N23PP/L28F

Plasmid

#118584

Purpose

Bacterial expression of E.coli DHFR N23PP and L28F mutant

Depositor

Stephen Benkovic

Publication

Liu et al Proc Natl Acad Sci U S A. 2013 Jun 18;11

Insert

DHFR

Expression

Bacterial

Mutation

L28 mutated to F28, N23 mutated to P23, a proline…

Promoter

T7

Available since

Nov. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pET151-Vps4(101-437)

Plasmid

#87735

Purpose

Bacterial expression of N-terminally truncated S. cerevisiae Vps4

Depositor

Chris Hill

Publication

Monroe et al Elife. 2017 Apr 5;6. pii: e24487. doi: 1

Insert

Vacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)

Tags

6xHis-V5-TEV

Expression

Bacterial

Mutation

deleted amino acids 1-100

Promoter

T7

Available since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pETconNK-TEM1(S70A,D179G)

Plasmid

#81166

Purpose

pETconNK with TEM1(S70A,D179G) beta-lactamase

Depositor

Timothy Whitehead

Publication

Klesmith et al Proc Natl Acad Sci U S A. 2017 Feb 14. p

Insert

TEM-1 beta-lactamase

Tags

c-myc

Expression

Bacterial and Yeast

Mutation

Deleted the sec transport tag. Plasmid encodes H2…

Promoter

GAL1 and gal1

Available since

Feb. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pET28a-Hook3 aa 1-160-GCN4

Plasmid

#74608

Purpose

Expresses artificially dimerized Hook domain of Hook3 in bacteria for purification

Depositor

Ron Vale

Publication

Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d

Insert

human Hook3 amino acids 1-160 (HOOK3 Human)

Tags

6x His, GCN4, Strep II, and superfolder GFP

Expression

Bacterial

Available since

Aug. 5, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pETM6-Pc4CL-PhCHS-MsCHI

Plasmid

#73396

Purpose

Expresses three enzymes to convert phenylpropanoic acids to flavanones in vivo

Depositor

Mattheos Koffas

Publication

Jones et al Metab Eng. 2016 Feb 6;35:55-63. doi: 10.

Inserts

Pc4CL
PhCHS
MsCHI

Expression

Bacterial

Promoter

T7

Available since

April 14, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET28-MRPP3-myc-His

Plasmid

#67864

Purpose

bacterial expression of PRORP (KIAA0391), full coding sequence (mitoch. form) + C-term. myc-His tag

Depositor

Walter Rossmanith

Publication

Holzmann et al Cell. 2008 Oct 31;135(3):462-74. doi: 10

Insert

MRPP3 (KIAA0391 Human)

Tags

His and myc

Expression

Bacterial

Mutation

Amino acid 46 of recombinant MRPP3 is preceded by…

Promoter

T7

Available since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pET28A-p53 (1-73) (P12A P13A)

Plasmid

#62066

Purpose

Expression of human p53 (residues 1-73) (P12A, P13A) in e. coli

Depositor

Gary Daughdrill

Publication

Borcherds et al Nat Chem Biol. 2014 Dec;10(12):1000-2. d

Insert

p53 (TP53 Human)

Tags

His

Expression

Bacterial

Mutation

Contains TAD residues 1-73; P12A, P13A

Promoter

T7

Available since

Feb. 23, 2015

Availability

Academic Institutions and Nonprofits only
human p53-(1-320)

Plasmid

#24864

Depositor

Cheryl Arrowsmith

Publication

Ayed et al Nat Struct Biol. 2001 Sep . 8(9):756-60.

Insert

p53 (TP53 Human)

Tags

His

Expression

Bacterial

Mutation

Contains aa 1-320

Available since

Aug. 23, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles
pHyperTEV60

Plasmid

#228496

Purpose

10xHis-SUMO (Smt3) tagged HyperTEV60 engineered protease (E. coli based protein expression)

Depositor

Richard Baker

Insert

TEV Protease

Tags

10xHis-SUMO(Smt3)

Expression

Bacterial

Promoter

T7 Promoter

Available since

Jan. 28, 2025

Availability

Academic Institutions and Nonprofits only

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JF001A

Plasmid

#53116

Purpose

expresses alpha hemolysin under the control of theophylline riboswitch

Depositor

Sheref Mansy

Publication

Lentini et al Nat Commun. 2014 May 30;5:4012. doi: 10.

Insert

theophylline riboswitch and alpha hemolysin

Expression

Bacterial

Promoter

T7 promoter

Available since

May 19, 2014

Availability

Academic Institutions and Nonprofits only

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pET21a asyn(41-140)

Plasmid

#240596

Purpose

Overexpress truncated alpha-Synuclein protein

Depositor

Jennifer Lee

Publication

McGlinchey et al Proc Natl Acad Sci U S A. 2021 Aug 31;11

Insert

aSyn (SNCA Human)

Tags

6xHis

Expression

Bacterial

Mutation

aa 41-140 only

Promoter

T7

Available since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pET21a asyn(36-140)

Plasmid

#240595

Purpose

Overexpress truncated alpha-Synuclein protein

Depositor

Jennifer Lee

Publication

McGlinchey et al Proc Natl Acad Sci U S A. 2021 Aug 31;11

Insert

aSyn (SNCA Human)

Tags

6xHis

Expression

Bacterial

Mutation

aa 36-140 only

Promoter

T7

Available since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pET30a-PmPPA-ET64

Plasmid

#234667

Purpose

Expression an ELP-tagged PmPPA enzyme

Depositor

Xianwei Liu

Publication

Wu et al Chembiochem. 2025 Jan 13:e202401005. doi

Insert

pyrophosphatase

Tags

ELP

Expression

Bacterial

Available since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

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pZH82 pET28a-Rai1

Plasmid

#231651

Purpose

Bacterial expression vector expressing yeast S. cerevisiae rai1, no tag

Depositor

Jesper Svejstrup

Publication

Han et al Mol Cell. 2023 Sep 21;83(18):3253-3267.e

Insert

rai1 (RAI1 Budding Yeast)

Expression

Bacterial

Available since

Feb. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaMIgG-14

Plasmid

#228994

Purpose

For bacterial expression of anti-mouse IgG nanobody LaMIgG-14, with pelB leader for periplasmic secretion, and C-term free cysteine and C-term 6xHIS tag.

Depositor

Michael Rout

Publication

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaMIgG-14

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaG94-5

Plasmid

#228965

Purpose

For bacterial expression of anti-GFP nanobody LaG94-5, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.

Depositor

Michael Rout

Publication

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaG94-5

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pETM6-Amp-T7-roGFP

Plasmid

#202464

Purpose

Expresses redox sensitive GFP (roGFP) with T7 induction

Depositor

Michael Lynch

Publication

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

reduction-oxidation sensitive green fluorescent protein

Tags

6X-His

Expression

Bacterial

Promoter

T7

Available since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pET21a-TEX264_wt-His6

Plasmid

#220214

Purpose

Bacterial expression of wild type TEX264, C-terminal His6 tag

Depositor

Kristijan Ramadan

Publication

Fielden et al Nat Commun. 2020 Mar 9;11(1):1274. doi:

Insert

TEX264 (TEX264 Human)

Tags

His6

Expression

Bacterial

Promoter

T7

Available since

Aug. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pETSUMO2_Vitiosangium-bGSDM-thrombin

Plasmid

#222099

Purpose

For expression of a mutant bacterial gasdermin (bGSDM) encoded by a Vitiosangium species with a thrombin protease cleavage site for controlled activation.

Depositor

Philip Kranzusch

Publication

Johnson et al Nature. 2024 Apr;628(8008):657-663. doi:

Insert

Vitiosangium-bGSDM-thrombin ORF

Tags

6xHis-SUMO2

Expression

Bacterial

Mutation

N-terminal methionine deleted and replaced by sin…

Promoter

T7

Available since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pETSUMO2_Nostoc-bGSDM-WELQ

Plasmid

#222100

Purpose

For expression of a mutant bacterial gasdermin (bGSDM) encoded by a Nostoc species with a WELQut protease cleavage site for controlled activation.

Depositor

Philip Kranzusch

Publication

Johnson et al Nature. 2024 Apr;628(8008):657-663. doi:

Insert

Nostoc-bGSDM-WELQ ORF

Tags

6xHis-SUMO2

Expression

Bacterial

Mutation

N-terminal methionine deleted and replaced by sin…

Promoter

T7

Available since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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