We narrowed to 77,030 results for: rest

1. pcDNA3.1 Flag YFP hNKCC1 I677C A734C (NT813)

   Plasmid

   #50868

   Purpose

   Expresses human NKCC1 I677C A734C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

   Depositor

   Biff Forbush

   Article

   Monette et al J Biol Chem. 2014 Jan 22.

   Insert

   human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
   

   Tags

   3x Flag and YFP

   Expression

   Mammalian

   Mutation

   I677C A734C in hNKCC1 in NT17

   Promoter

   CMV

   Available Since

   Feb. 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pUCM-AAVS1-TO-hNGN2

   Plasmid

   #105840

   Purpose

   Donor construct for introduction of NGN2 into AAVS1 safe harbor site and iPSC differentiation to cortical neuron

   Depositor

   Michael Ward

   Article

   Fernandopulle et al Curr Protoc Cell Biol. 2018 Jun;79(1):e5

   Inserts

   Neurogenin 2 (NEUROG2 Human)
   mCherry
   rtTA3G
   

   Use

   CRISPR and TALEN

   Promoter

   CAG, EF-1alpha, and TRE3G

   Available Since

   April 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pUCmini-iCAP-PHP.eC

   Plasmid

   #201903

   Purpose

   non-standard AAV2 rep-AAV-PHP.eC cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

   Depositor

   Viviana Gradinaru

   Article

   Shay et al Sci Adv. 2023 Apr 21;9(16):eadg6618. doi

   Insert

   Synthetic construct isolate AAV-PHP.eC VP1 gene
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   p41

   Available Since

   June 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. AAVS1 intron 1 gRNA as2

   Plasmid

   #132394

   Purpose

   Targets AAVS1 intron 1, gRNA: AGAACCAGAGCCACATTAAC, MLM3636 backbone

   Depositor

   Gerold Schmitt-Ulms

   Article

   Wang et al Bio Protoc. 2020 May 5;10(9):e3615. doi:

   Insert

   gRNA targeting AAVS1 intron 1 (PPP1R12C Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   May 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. TGFB1_pLX307

   Plasmid

   #98377

   Purpose

   Lentiviral expression of TGFB1

   Depositor

   William Hahn, Sefi Rosenbluh

   Article

   Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

   Insert

   TGFB1 (TGFB1 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Promoter

   E1Fa

   Available Since

   Aug. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. JUP_pLX307

   Plasmid

   #98347

   Purpose

   Lentiviral expression of JUP

   Depositor

   William Hahn, Sefi Rosenbluh

   Article

   Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

   Insert

   JUP (JUP Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Promoter

   E1Fa

   Available Since

   Aug. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA3.1 HA CFP hNKCC1 WT (NT15-H)

   Plasmid

   #49077

   Purpose

   Expresses human NKCC1 with an N-terminal 3xHA-CFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites. Native hNKCC1 amino acid sequence

   Depositor

   Biff Forbush

   Article

   Somasekharan et al PLoS One. 2013 Dec 5;8(12):e82060. doi:

   Insert

   human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
   

   Tags

   3x HA and mCerulean

   Expression

   Mammalian

   Mutation

   262 silent mutations from native hNKCC1

   Promoter

   CMV

   Available Since

   Nov. 19, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. MYC_pLX307

   Plasmid

   #98363

   Purpose

   Lentiviral expression of MYC

   Depositor

   William Hahn, Sefi Rosenbluh

   Article

   Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

   Insert

   MYC (MYC Human)
   

   Use

   Lentiviral

   Tags

   NA

   Expression

   Mammalian

   Promoter

   E1Fa

   Available Since

   Aug. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pODN-mNG-ANLN

   Plasmid

   #183834

   Purpose

   Repair template for the N-terminal tagging of anillin with mNeonGreen in human cells using CRISPR/Cas9.

   Depositor

   Alisa Piekny

   Article

   Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

   Insert

   ANLN homology arms with mNeonGreen-linker (ANLN Human)
   

   Use

   CRISPR; Donor template

   Tags

   mNeonGreen-linker

   Expression

   Mammalian

   Mutation

   Homology arms contain point mutations to remove t…

   Available Since

   May 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA5-eGFP-PC1-3HA

    Plasmid

    #108406

    Purpose

    Can be used to generate stable Flp-In cells with tetracycline-inducible human PC1 expression.

    Depositor

    Gregory Germino

    Article

    Lin et al Sci Rep. 2018 Feb 9;8(1):2743. doi: 10.1

    Insert

    PKD1 (PKD1 Human)
    

    Tags

    3HA and eGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pHR-BRD4ΔN-mCherry-sspB

    Plasmid

    #121968

    Purpose

    Expresses fusion of disordered protein BRD4(462-1362), fluorescent protein mCherry, and sspB which upon light activation binds to iLID.

    Depositor

    Clifford Brangwynne

    Article

    Shin et al Cell. 2018 Nov 29;175(6):1481-1491.e13.

    Insert

    BRD4 (BRD4 Human)
    

    Use

    Lentiviral

    Tags

    mCherry-sspB

    Expression

    Mammalian

    Mutation

    Deleted amino acids 1-461

    Promoter

    SFFV

    Available Since

    April 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. TUBG1_pLX307

    Plasmid

    #98376

    Purpose

    Lentiviral expression of TUBG1

    Depositor

    William Hahn, Sefi Rosenbluh

    Article

    Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

    Insert

    TUBG1 (TUBG1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Promoter

    E1Fa

    Available Since

    Aug. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLentiCRISPRv2-sgFXN-1

    Plasmid

    #246017

    Purpose

    All-in-one CRISPRko plasmid containing Cas9 and guide RNA targeting FXN

    Depositor

    Vamsi Mootha

    Article

    Ast et al Cell. 2019 May 30;177(6):1507-1521.e16.

    Insert

    Frataxin (FXN Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Oct. 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLentiCRISPRv2-sgFXN-2

    Plasmid

    #246018

    Purpose

    All-in-one CRISPRko plasmid containing Cas9 and guide RNA targeting FXN

    Depositor

    Vamsi Mootha

    Article

    Ast et al Cell. 2019 May 30;177(6):1507-1521.e16.

    Insert

    Frataxin (FXN Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Oct. 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pENTR-ABCC1_STOP

    Plasmid

    #221423

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    Giulio Superti-Furga, RESOLUTE Consortium

    Article

    Wolf et al Cell Chem Biol. 2024 Dec 24:S2451-9456(2

    Insert

    ABCC1 (ABCC1 Human)
    

    Expression

    Mammalian

    Available Since

    July 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pDONR223_SMURF2_WT

    Plasmid

    #82259

    Purpose

    Gateway Donor vector containing SMURF2 , part of the Target Accelerator Plasmid Collection.

    Depositor

    William Hahn, David Root, Jesse Boehm

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    SMURF2 (SMURF2 Human)
    

    Use

    Gateway entry vector

    Mutation

    WT

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pErCas12a EZ Clone

    Plasmid

    #132641

    Purpose

    Expresses ErCas12a and an sgRNA to target a region of interest

    Depositor

    Stephen Ekker

    Article

    Wierson et al CRISPR J. 2019 Dec;2(6):417-433. doi: 10

    Insert

    ErCas12a
    

    Use

    AAV and CRISPR

    Tags

    3x HA and SV40 NLS

    Expression

    Mammalian

    Mutation

    Q926R (Please see depositor comments) and BsaI si…

    Promoter

    CMV Promoter

    Available Since

    April 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLKO.1-puro-STING-sh5

    Plasmid

    #127647

    Purpose

    Knock-down of human STING

    Depositor

    Nicolas Manel

    Article

    Gratia et al J Exp Med. 2019 May 6;216(5):1199-1213.

    Insert

    STING shRNA (STING1 Human)
    

    Use

    Lentiviral

    Available Since

    July 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pENTR-li-p33_RS-CLIP

    Plasmid

    #104634

    Purpose

    Human Invariant chain with a CLIP replacement with in frame restriction sites (KpnI/XhoI)

    Depositor

    Sébastien Walchli

    Article

    Mensali et al Oncoimmunology. 2019 Jan 11;8(3):1558663

    Insert

    modified Invariant chain p33 (CD74 Human)
    

    Use

    Entry vector for gateway system

    Tags

    none

    Mutation

    CLIP domain was replaced by 2 restriction sites

    Available Since

    March 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDONR223_WNT5A_WT

    Plasmid

    #82240

    Purpose

    Gateway Donor vector containing WNT5A , part of the Target Accelerator Plasmid Collection.

    Depositor

    William Hahn, David Root, Jesse Boehm

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    WNT5A (WNT5A Human)
    

    Use

    Gateway entry vector

    Mutation

    WT

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge