We narrowed to 4,230 results for: erf

1. pLminP_Luc2P_RE57

   Plasmid

   #90402

   Purpose

   Type I Interferon - ISGF3G (STAT1, STAT2, IRF9) gene reporter

   Depositor

   Ramnik Xavier

   Article

   O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

   Insert

   Firefly luciferase
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   GR

   Available since

   Nov. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pBbS8c-ddcpf1-rfp

   Plasmid

   #153038

   Purpose

   For CRISPRi. Arabinose-inducible ddcpf1 gene with CRISPR array targeting the mrfp reporter gene.

   Depositor

   Nigel Scrutton

   Article

   Jervis et al Microb Biotechnol. 2021 Mar 12. doi: 10.

   Insert

   Ascpf1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   E993A

   Promoter

   Available since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCT310

   Plasmid

   #111140

   Purpose

   Bacterial expression of 6His-dCas9-3XFlag

   Depositor

   Robert Tjian

   Article

   Tsui et al Proc Natl Acad Sci U S A. 2018 Mar 20;11

   Insert

   dCas9
   

   Use

   Tags

   3X Flag and 6X His

   Expression

   Bacterial

   Mutation

   D10A - H840A

   Promoter

   T7

   Available since

   July 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pdCas9

   Plasmid

   #46569

   Purpose

   Expresses the tracrRNA, the dCas9 catalytic site mutant and a CRISPR array designed for the easy cloning of new spacers.

   Depositor

   Luciano Marraffini

   Article

   Bikard et al Nucleic Acids Res. 2013 Jun 12.

   Inserts

   tracrRNA
   dcas9
   CRISPR array
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   D10A, H840A

   Promoter

   Available since

   Aug. 27, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLV hUbC-dCas9 KRAB-T2A-GFP

   Plasmid

   #67620

   Purpose

   Co-expressed human optimized S. pyogenese dCas9 fused to KRAB repressor domain and GFP

   Depositor

   Charles Gersbach

   Article

   Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

   Insert

   humanized dead Cas9 KRAB T2A GFP
   

   Use

   CRISPR and Lentiviral

   Tags

   Flag

   Expression

   Mutation

   D10A and H840A

   Promoter

   Available since

   March 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pHAGE EF1α dCas9-KRAB

   Plasmid

   #50919

   Purpose

   Constitutive dCas9-KRAB 2nd generation lentiviral expression vector

   Depositor

   Scot Wolfe, Rene Maehr

   Article

   Kearns et al Development. 2014 Jan;141(1):219-23. doi

   Insert

   dCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   3xHA and KRAB

   Expression

   Mammalian

   Mutation

   D10A, H840A

   Promoter

   EF1alpha

   Available since

   March 28, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pSR59.4

   Plasmid

   #63175

   Purpose

   Expresses OmpR constitutively from PompB97 and sfGFP under the PompF146 promoter

   Depositor

   Jeffrey Tabor

   Article

   Schmidl et al ACS Synth Biol. 2014 Nov 21;3(11):820-31

   Inserts

   OmpR (ompR )
   sfGFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET28-His6-GFP-C-LACE

   Plasmid

   #133913

   Purpose

   Bacterial expression of His6-GFP-C-LACE

   Depositor

   Jeffrey Bode

   Article

   Hofmann et al Nat Chem. 2020 Nov;12(11):1008-1015. doi

   Insert

   superfolder green fluorescent protein
   

   Use

   Tags

   LACE tag (GSGPRKVIKMESEE) and TEV-cleavable His6-…

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pHAGE TRE dCas9

   Plasmid

   #50915

   Purpose

   Tet-regulatable dCas9 lentiviral expression vector

   Depositor

   Scot Wolfe, Rene Maehr

   Article

   Kearns et al Development. 2014 Jan;141(1):219-23. doi

   Insert

   dCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D10A, H840A

   Promoter

   TRE

   Available since

   Feb. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. SpyTag-sfGFP

    Plasmid

    #184226

    Purpose

    Expresses SpyTag-superfolder GFP in the bacterial cytoplasm. SpyTag enables covalent reaction with SpyCatcher.

    Depositor

    Mark Howarth

    Article

    Vester et al Nat Commun. 2022 Jun 28;13(1):3714. doi:

    Insert

    SpyTag-sfGFP
    

    Use

    Tags

    His6, SpyTag, and TEV protease cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T5

    Available since

    June 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pYPQ153

    Plasmid

    #69304

    Purpose

    Gateway entry vector with pco-dCas9-3X(SRDX)

    Depositor

    Yiping Qi

    Article

    Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

    Insert

    pco-dCas9-3X(SRDX) (Plant codon-optimized)
    

    Use

    CRISPR

    Tags

    2XFLAG

    Expression

    Mutation

    SRDX (X3) repressor fusion; NLS fusion

    Promoter

    Available since

    Oct. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLenti_dCas9-KRAB-MeCP2_Blast

    Plasmid

    #221501

    Purpose

    Lentiviral construct for expression of the dCas9-KRAB-MeCP2 CRISPRi vector

    Depositor

    Matthew Meyerson

    Article

    Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

    Insert

    dCas9-KRAB-MeCP2-T2A-Blast
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

    Promoter

    CMV

    Available since

    July 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pdCas9-NED

    Plasmid

    #109358

    Purpose

    Mammalian vector for expressing effector domains fused to dCas9.

    Depositor

    Antal Kiss

    Article

    Goubert et al Methods Mol Biol. 2018;1767:395-415. doi

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    dCas9, SV40 NLS, 3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLV hUbC-dCas9-T2A-GFP

    Plasmid

    #53191

    Purpose

    Co-expresses human optimized S. pyogenes dCas9 and GFP

    Depositor

    Charles Gersbach

    Article

    Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

    Insert

    humanized dead Cas9 T2A GFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    D10A and H840A

    Promoter

    hUbC

    Available since

    June 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pHR-PGK-ABI-dCas9-P2A-mcherry

    Plasmid

    #121513

    Purpose

    expression of AB1-fused-dcas9 plasmids

    Depositor

    Stanley Qi

    Article

    Wang et al Cell. 2018 Nov 15;175(5):1405-1417.e14.

    Insert

    dCas9
    

    Use

    Lentiviral

    Tags

    AB1 and P2A-mcherry

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    Feb. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMSP3545-dCas9Str

    Plasmid

    #153516

    Purpose

    Expresses dCas9str under nisin-inducible nisA promoter in pMSP3545 backbone

    Depositor

    Kimberly Kline

    Article

    Afonina et al mBio. 2020 Oct 20;11(5):e01101-20. doi:

    Insert

    dCas9Str
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    nisA

    Available since

    July 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET28a-PACT

    Plasmid

    #50352

    Purpose

    bacterial expression of PACT

    Depositor

    Kumiko Ui-Tei

    Article

    Takahashi et al PLoS One. 2013 May 2;8(5):e63434. doi: 1

    Insert

    PACT (PRKRA Human)
    

    Use

    Tags

    His and Myc

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMJ841

    Plasmid

    #39318

    Depositor

    Jennifer Doudna

    Article

    Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    His6 and MBP

    Expression

    Bacterial

    Mutation

    Aspartate 10 to Alanine (D10A) and Histidine 840 …

    Promoter

    T7

    Available since

    Aug. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET28a-Hook3 aa 1-552

    Plasmid

    #74614

    Purpose

    Expresses a truncation of Hook3 in bacteria for purification

    Depositor

    Ron Vale

    Article

    Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d

    Insert

    human Hook3 amino acids 1-552 (HOOK3 Human)
    

    Use

    Tags

    6x His, Strep II, and superfolder GFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET28a-FIP3

    Plasmid

    #74753

    Purpose

    Expressed sfGFP-tagged FIP3 in E. coli for protein production

    Depositor

    Ron Vale

    Article

    Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554

    Insert

    Rab11 family interacting protein 3 (RAB11FIP3 Human)
    

    Use

    Tags

    6x His, Strep II, and superfolder GFP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge