We narrowed to 6,705 results for: hwa

1. pRevTRE MKL1 S449A/T450A/S454A

   Plasmid

   #19847

   Depositor

   Ron Prywes

   Article

   Muehlich et al Mol Cell Biol. 2008 Oct;28(20):6302-13.

   Insert

   MKL1 S449A/T450A/S454A (MRTFA Human)
   

   Use

   Retroviral

   Tags

   3xFLAG

   Mutation

   changed Serine 449, Threonine 450 and Serine 454 …

   Available Since

   Dec. 16, 2008

   Availability

   Academic Institutions and Nonprofits only

2. B1 Integrin

   Plasmid

   #186137

   Purpose

   For expression of recombinant beta1 integrin cytosolic domain in bacteria

   Depositor

   Michael Rosen

   Article

   Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

   Insert

   ITGB1 (ITGB1 Human)
   

   Tags

   MBP and untranslated His6 after stop codon

   Expression

   Bacterial

   Promoter

   Lac

   Available Since

   June 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMSCV-PIG-Lin28b

   Plasmid

   #64795

   Purpose

   Expresses human Lin28b in mammalian cells

   Depositor

   Joshua Mendell

   Article

   Chang et al Proc Natl Acad Sci U S A. 2009 Mar 3;106

   Insert

   Lin28b (LIN28B Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   Nov. 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. SNW1

   Plasmid

   #156034

   Purpose

   For use in RBP tethering screen

   Depositor

   Eugene Yeo

   Article

   Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

   Insert

   SNW1 (SNW1 Human)
   

   Tags

   V5-MCP

   Expression

   Mammalian

   Mutation

   none

   Promoter

   EF-1a

   Available Since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. Y587E EphA2 pcDNA3

   Plasmid

   #102730

   Purpose

   Expression of EphA2 in mammalian cells

   Depositor

   Jin Chen

   Article

   Fang et al J Biol Chem. 2008 Jun 6;283(23):16017-26

   Insert

   EphA2 (Epha2 Mouse)
   

   Expression

   Mammalian

   Mutation

   Y587E (TAT > GAG)

   Promoter

   CMV

   Available Since

   Dec. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. AdMKP-3 S197A

   Plasmid

   #131522

   Purpose

   Expresses MKP-3 with S197A mutation in mammalian cells

   Depositor

   Haiyan Xu

   Article

   Feng et al Mol Cell Endocrinol. 2012 Sep 25;361(1-2

   Insert

   Mitogen-activated protein kinase phosphatase 3 (Dusp6 Mouse)
   

   Use

   Adenoviral

   Tags

   No

   Mutation

   changed Serine 197 to Alanine

   Promoter

   CMV

   Available Since

   Oct. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. Hy_pMT Flag-Hel25E K91N

   Plasmid

   #110121

   Purpose

   Expresses Flag-tagged D. melanogaster Hel25E with K91N mutation that greatly reduces helicase activity

   Depositor

   Jeremy Wilusz

   Article

   Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

   Insert

   Hel25E (Hel25E Fly)
   

   Tags

   Flag

   Expression

   Insect

   Mutation

   K91N mutation

   Promoter

   Metallothionein Promoter (pMT)

   Available Since

   Sept. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. Hy_pMT Flag-Hel25E D193E

   Plasmid

   #110120

   Purpose

   Expresses Flag-tagged D. melanogaster Hel25E with D193E mutation that greatly reduces ATP binding affinity

   Depositor

   Jeremy Wilusz

   Article

   Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

   Insert

   Hel25E (Hel25E Fly)
   

   Tags

   Flag

   Expression

   Insect

   Mutation

   D193E mutation

   Promoter

   Metallothionein Promoter (pMT)

   Available Since

   Sept. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pKS7107

   Plasmid

   #89051

   Purpose

   Expresses Nm crRNA, Nm tracrRNA and human codon-optimized NmCas9

   Depositor

   Ervin Welker

   Article

   Toth et al Biol Direct. 2016 Sep 14;11:46. doi: 10.

   Inserts

   Nm crRNA
   Nm tracrRNA
   hNmCas9
   

   Use

   CRISPR

   Tags

   HA tag, NLS, and SV40 NLS

   Promoter

   EF1a and human U6

   Available Since

   April 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEGFP-Cln6

    Plasmid

    #78109

    Purpose

    mammalian expression of mouse Cln6 with C terminal EGFP fusion

    Depositor

    Thomas Braulke

    Article

    Thelen et al PLoS One. 2012;7(4):e35493. doi: 10.1371

    Insert

    Cln6 (Cln6 Mouse)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 20, 2016

    Availability

    Academic Institutions and Nonprofits only

11. Opto-GPR27

    Plasmid

    #106013

    Purpose

    Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR27; Opto-GPR27; B3)

    Depositor

    Harald Janovjak

    Article

    Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

    Insert

    Opto-GPR27 (GPR27 Human, Bovine)
    

    Tags

    Rhodopsin-1D4 and VSV-G

    Expression

    Mammalian

    Mutation

    Chimeric receptor protein

    Promoter

    CMV

    Available Since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMXs-hs-3xHA-deltaRING-LIN-41

    Plasmid

    #52718

    Purpose

    retroviral expression of human LIN-41 / TRIM71 containing a RING domain deletion and 3 N-terminal HA tags

    Depositor

    Shinya Yamanaka

    Article

    Worringer et al Cell Stem Cell. 2014 Jan 2;14(1):40-52.

    Insert

    LIN-41 (TRIM71 Human)
    

    Use

    Retroviral

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    deleted amino acids 12-91

    Available Since

    June 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLKO.1-shRBFOX1-3261

    Plasmid

    #115458

    Purpose

    Constitutive lentiviral expression

    Depositor

    William Hahn

    Article

    Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

    Insert

    shRBFOX1-3261 (RBFOX1 Human)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    Sept. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLKO.1-shRBFOX1-3260

    Plasmid

    #115457

    Purpose

    Constitutive lentiviral expression

    Depositor

    William Hahn

    Article

    Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

    Insert

    shRBFOX1-3260 (RBFOX1 Human)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    Sept. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. Y921F EphA2 pcDNA3

    Plasmid

    #102736

    Purpose

    Expression of EphA2 in mammalian cells

    Depositor

    Jin Chen

    Article

    Fang et al J Biol Chem. 2008 Jun 6;283(23):16017-26

    Insert

    EphA2 (Epha2 Mouse)
    

    Expression

    Mammalian

    Mutation

    Y921F (TAC > TTC)

    Promoter

    CMV

    Available Since

    Nov. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. PRPF40A

    Plasmid

    #155851

    Purpose

    For use in RBP tethering screen

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    PRPF40A (PRPF40A Human)
    

    Tags

    V5-MCP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    EF-1a

    Available Since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pUC19-G2ZYP2

    Plasmid

    #103110

    Purpose

    Cas9 coding gene template with optimized sequence for human codon usage

    Depositor

    Duhee Bang

    Article

    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55

    Insert

    G2ZYP2(Cas9 coding gene from Ralstonia syzygii R24)
    

    Use

    CRISPR; Cloning vector

    Mutation

    human codon-optimized

    Available Since

    April 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pUC19-Q927P4

    Plasmid

    #103131

    Purpose

    Cas9 coding gene template with optimized sequence for human codon usage

    Depositor

    Duhee Bang

    Article

    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55

    Insert

    Q927P4(Cas9 coding gene from Listeria innocua serovar 6a (strain CLIP 11262))
    

    Use

    CRISPR; Cloning vector

    Mutation

    human codon-optimized

    Available Since

    April 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRD408

    Plasmid

    #178199

    Purpose

    Expression of H-NS from its natural promoter

    Depositor

    Remus Dame

    Article

    Erkelens et al Nucleic Acids Res. 2022 Nov 21. pii: 683

    Insert

    phns + hns (hns Escherichia coli)
    

    Expression

    Bacterial

    Promoter

    hns promoter

    Available Since

    Nov. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pPPAT-11AA-2XStrep

    Plasmid

    #125680

    Purpose

    expresses human PPAT protein, with first 11 residues removed, with a C-terminal 2XStrep affinity tag

    Depositor

    Stephen Benkovic

    Article

    Liu et al J Proteome Res. 2019 May 3;18(5):2078-20

    Insert

    PPAT (PPAT Human)
    

    Tags

    2X Strep-Tag II

    Expression

    Mammalian

    Mutation

    the first eleven residues have been removed

    Promoter

    CMV

    Available Since

    May 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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