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pAAV-CAG-UnaG-P2A-FusionRed

Plasmid

#191109

Purpose

Mammalian cell line expression plasmids for production of UnaG and FusionRed proteins

Depositor

Kiryl Piatkevich

Article

Liang et al Front Bioeng Biotechnol. 2022 Oct 17;10:

Insert

UnaG

Use

AAV

Tags

FusionRed

Expression

Mammalian

Mutation

See depositor comments below

Promoter

chicken β-actin promoter

Available Since

Nov. 6, 2024

Availability

Academic Institutions and Nonprofits only

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DR5rev::erRFP

Plasmid

#61011

Purpose

Auxin signalling reporter (DR5rev) driving RFP (ER-targeted)

Depositor

Alexis Maizel

Article

Marin et al Plant Cell. 2010 Apr;22(4):1104-17. doi:

Insert

DR5rev::erRFP:35S_term

Expression

Plant

Promoter

DR5rev::erRFP:35Sterm

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pNIFV

Plasmid

#111085

Purpose

pNIFV is a vector plasmid with mutations in the cis-acting region, to prevent correction of the integrase by recombination when used with pCiPS-NIFV to produce Non-Integrating Foamy Virus vectors

Depositor

David Russell

Article

Deyle et al J Virol. 2010 Sep;84(18):9341-9. doi: 10

Insert

empty backbone/deleted foamy vector, same as pΔΦ with mutations in cis-acting pol region

Use

Deleted foamy virus

Mutation

at nt 2334 A changed to T, at nt 2495 A changed t…

Promoter

CMV

Available Since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti_CMV:Flag-GpNLuc

Plasmid

#208840

Purpose

Expresses FLAG epitope-tagged GpNLuc "Green" BRET reporter in mammalian cells.

Depositor

Antonio Amelio

Article

Labra et al iScience. 2024 Aug 8;27(9):110655. doi:

Insert

GpNLuc LumiFluor

Use

Lentiviral

Tags

1xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-AID

Plasmid

#61926

Purpose

Expression of human AID tagged with EGFP

Depositor

Jon Houseley

Article

Lambert et al PLoS One. 2013 Dec 4;8(12):e82110. doi:

Insert

AICDA (AICDA Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

April 21, 2015

Availability

Academic Institutions and Nonprofits only

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pLenti-NRCH-PE2-BSD

Plasmid

#191101

Purpose

Lentiviral expression plasmid of NRCH-PE prime editor with P2A-BSD marker

Depositor

Hyongbum Kim

Article

Yu et al Cell. 2023 Apr 21:S0092-8674(23)00331-8.

Insert

NRCH-PE2-BSD

Use

CRISPR and Lentiviral

Tags

SV40 NLS

Expression

Mammalian

Promoter

EF-1a

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV7.1_3xFLAG-WWC2

Plasmid

#172995

Purpose

constitutive expression of FLAG-tagged WWC2

Depositor

Michael Wehr

Article

Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

Insert

3xFLAG-WWC2 (WWC2 Human)

Tags

3xFLAG

Expression

Mammalian

Available Since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti_CMV:Flag-OgNLuc

Plasmid

#208851

Purpose

Expresses FLAG epitope-tagged OgNLuc "Orange" BRET reporter in mammalian cells.

Depositor

Antonio Amelio

Article

Labra et al iScience. 2024 Aug 8;27(9):110655. doi:

Insert

OgNLuc LumiFluor

Use

Lentiviral

Tags

1xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pGCP123-GFP_g1

Plasmid

#153518

Purpose

sgRNA to GFP gene (NT) under nisin-inducible nisA promoter, barcoded

Depositor

Kimberly Kline

Article

Afonina et al mBio. 2020 Oct 20;11(5):e01101-20. doi:

Insert

PnisA-sgRNA(GFP_g1)_dCas9 scaffold (barcoded)

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Available Since

July 28, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti_CMV:Flag-GeNL

Plasmid

#208841

Purpose

Expresses FLAG epitope-tagged GeNL "Green" BRET reporter in mammalian cells.

Depositor

Antonio Amelio

Article

Labra et al iScience. 2024 Aug 8;27(9):110655. doi:

Insert

GeNL Nanolantern

Use

Lentiviral

Tags

1xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 4, 2024

Availability

Academic Institutions and Nonprofits only

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GDAP2-c005

Plasmid

#173110

Purpose

Expression of recombinant fusion protein in E. coli

Depositor

Lari Lehtiö

Article

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Insert

GDAP2 (GDAP2 Human)

Tags

mCerulean

Expression

Bacterial

Mutation

M1-N231

Available Since

Nov. 15, 2021

Availability

Academic Institutions and Nonprofits only

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STIMATE-mCherry

Plasmid

#70676

Purpose

Expresses STIMATE or TMEM110 in mammalian cells

Depositor

Yubin Zhou

Article

Jing et al Nat Cell Biol. 2015 Oct;17(10):1339-47.

Insert

STIMATE (TMEM110 Human)

Tags

mcherry

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only
pCMV7.1_3xFLAG-WWC3

Plasmid

#172996

Purpose

constitutive expression of FLAG-tagged WWC3

Depositor

Michael Wehr

Article

Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

Insert

3xFLAG-WWC3 (WWC3 Human)

Tags

3xFLAG

Expression

Mammalian

Available Since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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k3(long) luciferase

Plasmid

#11110

Depositor

Anjana Rao

Article

Tsai et al Mol Cell Biol. 1996 Feb . 16(2):459-67.

Insert

k3(L) (TNF Human)

Tags

luciferase

Expression

Bacterial

Available Since

April 24, 2006

Availability

Academic Institutions and Nonprofits only
Tmem214 pmRFP-N2 (1348)

Plasmid

#62047

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Wilkie et al Mol Cell Proteomics. 2011 Jan;10(1):M110

Insert

Tmem214 (TMEM214 Human)

Tags

mRFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 23, 2015

Availability

Academic Institutions and Nonprofits only
Tmem214 pEGFP-N2 (1072)

Plasmid

#62026

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Wilkie et al Mol Cell Proteomics. 2011 Jan;10(1):M110

Insert

Tmem214 (TMEM214 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only
5UTR_MpUBE2

Plasmid

#136110

Purpose

5UTR for cloning with PROM_MpUEB2. PROM+5UTR_MpUBE2 has ubiquitous expression in early stages of gemmae development.

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

5UTR_MpUBE2 Mapoly0106s0019

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pANTO5

Plasmid

#131106

Purpose

To generate an integrative plasmid carrying the TetR/Pip-OFF system, but not the integrase

Depositor

Riccardo Manganelli

Article

Boldrin et al Sci Rep. 2019 Apr 8;9(1):5783. doi: 10.1

Insert

tetR gene by pFRA61 (Boldrin et al 2010)

Expression

Bacterial

Available Since

Dec. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pDEST-FRT-TO-GFP-PALB2 KR

Plasmid

#71107

Purpose

mammalian expression of GFP-PALB2 mutant. (first 8 Lysine residues mutated to Arginine)

Depositor

Daniel Durocher

Article

Orthwein et al Nature. 2015 Dec 9. doi: 10.1038/nature1

Insert

PALB2 (PALB2 Human)

Tags

GFP

Expression

Mammalian

Mutation

first 8 Lysine residues mutated to Arginine (K14-…

Promoter

CMV/TO

Available Since

Dec. 2, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pFUGW-RapACR-EYFP

Plasmid

#111075

Purpose

Fast anion channelrhodopsin (current half-decay time 3-10 ms depending on voltage and light intensity) for time-resolved inhibition of neuronal spiking

Depositor

John Spudich

Article

Govorunova et al eNeuro. 2018 Jul 10;5(3). pii: eN-MNT-01

Insert

Anion channelrhodopsin RapACR

Use

Lentiviral

Tags

EYFP

Available Since

June 4, 2018

Availability

Academic Institutions and Nonprofits only

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