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pcDNA-Flag-Kdm4b-pA

Plasmid

#104486

Purpose

Express mouse Kdm4b in mammalian cells. It has a T7 promoter for in vitro transcription. Since polyA is encoded on the vector, polyA-tailing is unnecessary.

Depositor

Yi Zhang

Publication

Inoue et al Genes Dev. 2017 Oct 1;31(19):1927-1932.

Insert

Kdm4b (Kdm4b Mouse)

Tags

Flag epitope tag

Expression

Mammalian

Available since

Dec. 19, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA-Flag-Kdm4b-pA

Plasmid

#104486

Purpose

Express mouse Kdm4b in mammalian cells. It has a T7 promoter for in vitro transcription. Since polyA is encoded on the vector, polyA-tailing is unnecessary.

Depositor

Yi Zhang

Publication

Inoue et al Genes Dev. 2017 Oct 1;31(19):1927-1932.

Insert

Kdm4b (Kdm4b Mouse)

Tags

Flag epitope tag

Expression

Mammalian

Available since

Dec. 19, 2017

Availability

Academic Institutions and Nonprofits only

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pCXN2-FLAG-AT2R-CFP

Plasmid

#101660

Purpose

Expresses AT2R with FLAG tag and CFP in mammalian cells.

Depositor

Yusuke Ohba

Publication

Inuzuka et al Sci Rep. 2016 Feb 9;6:21613. doi: 10.103

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Angiotensin II type 2 receptor (AGTR2 Human)

Tags

ECFP and FLAG

Expression

Mammalian

Promoter

CAG promoter

Available since

Dec. 5, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCXN2-FLAG-AT2R-CFP

Plasmid

#101660

Purpose

Expresses AT2R with FLAG tag and CFP in mammalian cells.

Depositor

Yusuke Ohba

Publication

Inuzuka et al Sci Rep. 2016 Feb 9;6:21613. doi: 10.103

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Angiotensin II type 2 receptor (AGTR2 Human)

Tags

ECFP and FLAG

Expression

Mammalian

Promoter

CAG promoter

Available since

Dec. 5, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pKIR1.0

Plasmid

#86409

Purpose

CRISPR/Cas9 in Arabidopsis with seed a fluorescent reporter.

Depositor

Tetsuya Higashiyama

Publication

Tsutsui et al Plant Cell Physiol. 2016 Nov 17. pii: pc

Insert

human-codon-optimized SpCas9

Use

CRISPR

Tags

FLAG and NLS

Promoter

AtRPS5A

Available since

Feb. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pKIR1.0

Plasmid

#86409

Purpose

CRISPR/Cas9 in Arabidopsis with seed a fluorescent reporter.

Depositor

Tetsuya Higashiyama

Publication

Tsutsui et al Plant Cell Physiol. 2016 Nov 17. pii: pc

Insert

human-codon-optimized SpCas9

Use

CRISPR

Tags

FLAG and NLS

Promoter

AtRPS5A

Available since

Feb. 28, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
LVXN-Neo-NSD2-E1099K

Plasmid

#86011

Purpose

express NSD2 E1099K mutant in mammalian cells

Depositor

Darrin Stuart

Publication

Jaffe et al Nat Genet. 2013 Nov;45(11):1386-91. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

NSD2 E1099K mutant (NSD2 Human)

Tags

Flag tag D Y K D D D D K

Expression

Mammalian

Mutation

E1099K; K1002R (please see depositor comments bel…

Promoter

CMV

Available since

Jan. 13, 2017

Availability

Academic Institutions and Nonprofits only
LVXN-Neo-NSD2-E1099K

Plasmid

#86011

Purpose

express NSD2 E1099K mutant in mammalian cells

Depositor

Darrin Stuart

Publication

Jaffe et al Nat Genet. 2013 Nov;45(11):1386-91. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

NSD2 E1099K mutant (NSD2 Human)

Tags

Flag tag D Y K D D D D K

Expression

Mammalian

Mutation

E1099K; K1002R (please see depositor comments bel…

Promoter

CMV

Available since

Jan. 13, 2017

Availability

Academic Institutions and Nonprofits only
pAG416-Gal-PR50

Plasmid

#84901

Purpose

induce expression of dipeptide repeat PR in yeast

Depositor

Aaron Gitler

Publication

Jovicic et al Nat Neurosci. 2015 Sep;18(9):1226-9. doi

Insert

PR50 (C9orf72 Synthetic)

Tags

FLAG and Myc

Expression

Yeast

Promoter

GAL1

Available since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pAG416-Gal-PR50

Plasmid

#84901

Purpose

induce expression of dipeptide repeat PR in yeast

Depositor

Aaron Gitler

Publication

Jovicic et al Nat Neurosci. 2015 Sep;18(9):1226-9. doi

Insert

PR50 (C9orf72 Synthetic)

Tags

FLAG and Myc

Expression

Yeast

Promoter

GAL1

Available since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pBiex-1 Myosin VI HMM_eGFP

Plasmid

#82714

Purpose

Expresses Myosin VI HMM with a C-terminal eGFP.

Depositor

Sivaraj Sivaramakrishnan

Publication

Sommese et al Protein Sci. 2016 Aug 18. doi: 10.1002/p

Insert

Myosin VI

Tags

FLAG purification tag and eGFP

Expression

Bacterial and Insect

Promoter

T7 Promoter

Available since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pBiex-1 Myosin VI HMM_eGFP

Plasmid

#82714

Purpose

Expresses Myosin VI HMM with a C-terminal eGFP.

Depositor

Sivaraj Sivaramakrishnan

Publication

Sommese et al Protein Sci. 2016 Aug 18. doi: 10.1002/p

Insert

Myosin VI

Tags

FLAG purification tag and eGFP

Expression

Bacterial and Insect

Promoter

T7 Promoter

Available since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pRK5-FLAG-FNIP1

Plasmid

#72292

Purpose

overexpression

Depositor

David Sabatini

Publication

Tsun et al Mol Cell. 2013 Nov 21;52(4):495-505. doi

Insert

FNIP1 (FNIP1 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only
pRK5-FLAG-FNIP1

Plasmid

#72292

Purpose

overexpression

Depositor

David Sabatini

Publication

Tsun et al Mol Cell. 2013 Nov 21;52(4):495-505. doi

Insert

FNIP1 (FNIP1 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA3-FLAG-MTOR-E1799K

Plasmid

#69009

Purpose

activating MTOR mutation

Depositor

David Sabatini

Publication

Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

MTOR-E1799K (MTOR Human)

Tags

FLAG

Expression

Mammalian

Mutation

change Glutamate 1799 to Lysine

Available since

Dec. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-MTOR-E1799K

Plasmid

#69009

Purpose

activating MTOR mutation

Depositor

David Sabatini

Publication

Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

MTOR-E1799K (MTOR Human)

Tags

FLAG

Expression

Mammalian

Mutation

change Glutamate 1799 to Lysine

Available since

Dec. 17, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
GPR110-Tango

Plasmid

#66302

Purpose

Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

Depositor

Bryan Roth

Publication

Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

GPR110 (ADGRF1 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available since

Sept. 17, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
GPR110-Tango

Plasmid

#66302

Purpose

Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

Depositor

Bryan Roth

Publication

Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

GPR110 (ADGRF1 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available since

Sept. 17, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
PTGER2-Tango

Plasmid

#66484

Purpose

Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

Depositor

Bryan Roth

Publication

Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

PTGER2 (PTGER2 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

Enlarge
PTGER2-Tango

Plasmid

#66484

Purpose

Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

Depositor

Bryan Roth

Publication

Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

PTGER2 (PTGER2 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

Enlarge