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We narrowed to 4,700 results for: golden gate

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Showing: 3381 - 3400 of 4700 results

  7. pShoHELIX-sgRNA_entry (BO3)

    Plasmid
    #181784
    Purpose
    Expresses ShoHELIX containing a nicking I-AniI fusion to ShoTnsB. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).
    Depositor
    Benjamin Kleinstiver
    Article
    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Insert
    nAniI-ShoTnsB, ShoTnsC, ShoTniQ, ShoCas12k
    Expression
    Bacterial
    Mutation
    nAniI = K227M, F80K, L232K
    Promoter
    Lac and J23119
    Available Since
    March 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    181784-plasmid-map-sequence-id-358224

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  8. pICH-nuc-roGFP2-GPXC71S

    Plasmid
    #191760
    Purpose
    Ti plasmid pICH86988 harbouring nuc-roGFP2-GPXC71S -a nuclear-targeted control roGFP2 fusion not responsive to peroxides used alongside plasmids 191759 and 191758
    Depositor
    Philip Mullineaux
    Article
    Exposito-Rodriguez et al bioRxiv 2024.01.18.576236
    Insert
    nuc-roGFP2-GPXC71S
    Use
    Binary agrobacterium ti plasmid
    Expression
    Plant
    Mutation
    GPX has no peroxidatic Cysteine 71, which is repl…
    Promoter
    CaMV 35S
    Available Since
    March 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    191760-plasmid-map-sequence-id-378856

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  9. pEPOR1CB0013

    Plasmid
    #117549
    Purpose
    Level 1 Golden Gate Cassette: LbCas12a expression cassette for dicotyledonous plants
    Depositor
    Nicola Patron
    Article
    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do
    Insert
    2x35S+5'UTR OMEGA (pICH51288) + LbCas12a (with stop codon) (pEPOR0SP0007)+ Nos (pICH41421)
    Expression
    Plant
    Promoter
    2x35S+5'UTR OMEGA (pICH51288)
    Available Since
    Nov. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    117549-plasmid-map-sequence-id-228087

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  12. pTU2S-a (colE1 origin)

    Plasmid
    #74091
    Purpose
    Secondary module (BpiI) site in pTU2-a backbone with colE1 (medium-copy) origin of replication
    Depositor
    Paul Freemont
    Article
    Moore et al ACS Synth Biol. 2016 May 2.
    Insert
    Golden Gate destination vector - Level 2, 2 TU's with secondary module and colE1 origin
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    None
    Available Since
    April 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    74091-plasmid-map-sequence-id-138318

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  16. pICH-chl-roGFP2-GPXC71S

    Plasmid
    #191703
    Purpose
    Ti plasmid pICH86988 harbouring roGFP2-GPXC71S -a plastid-targeted control roGFP2 fusion not responsive to peroxides used alongside plasmids 191699 and 191701
    Depositor
    Philip Mullineaux
    Article
    Exposito-Rodriguez et al bioRxiv 2024.01.18.576236
    Insert
    chl-redox(ro)GFP2-GPXC71S
    Use
    Binary agrobacterium ti plasmid
    Expression
    Plant
    Mutation
    Peroxidatic Cysteine 71 replaced with Serine
    Promoter
    CaMV 35S
    Available Since
    March 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    191703-plasmid-map-sequence-id-378859

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  18. pICSL11023

    Plasmid
    #117542
    Purpose
    Level 1 Golden Gate Cassette: Cas9 expression cassette for dicotyledonous plants
    Depositor
    Nicola Patron
    Article
    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do
    Insert
    2x35S+5'UTR OMEGA (pICH51288) + SpCas9-h (no stop codon)(pICSL90005) + YFP (pICSL50005)+ Nos (pICH41421)
    Expression
    Plant
    Promoter
    2x35S_OMEGA(pICH51288)
    Available Since
    Nov. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    117542-plasmid-map-sequence-id-228061

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Showing: 3381 - 3400 of 4700 results