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SYRAP_pDONR201

Plasmid

#249814

Purpose

Encodes SYRAP (RAP2.12-GAL4STE12), Gateway compatible as Donor plasmid

Depositor

Francesco Licausi

Article

Lavilla-Puerta et al bioRxiv 2025.09.01.673502

Insert

RAP2.12-GAL4STE (RAP2.12 Budding Yeast, Mustard Weed)

Use

Synthetic Biology

Tags

GAL4-STE12

Expression

Plant and Yeast

Available Since

Feb. 5, 2026

Availability

Academic Institutions and Nonprofits only

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pRS303N-Padh-OsTIR1(F74G)

Plasmid

#247626

Purpose

Expression of OsTIR1

Depositor

Sebastian Schuck

Article

Hubbe et al Mol Biol Cell. 2026 Feb 1;37(2):mr2. doi

Insert

OsTIR1

Expression

Yeast

Mutation

F74G

Promoter

ADH1

Available Since

Dec. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pRS303K-Prnr1-OsTIR1(F74G)

Plasmid

#247619

Purpose

Expression of OsTIR1

Depositor

Sebastian Schuck

Article

Hubbe et al Mol Biol Cell. 2026 Feb 1;37(2):mr2. doi

Insert

OsTIR1

Expression

Yeast

Mutation

F74G

Promoter

RNR1

Available Since

Dec. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pRS303K-Padh-OsTIR1(F74G)

Plasmid

#247618

Purpose

Expression of OsTIR1

Depositor

Sebastian Schuck

Article

Hubbe et al Mol Biol Cell. 2026 Feb 1;37(2):mr2. doi

Insert

OsTIR1

Expression

Yeast

Mutation

F74G

Promoter

ADH1

Available Since

Dec. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pRS303K-Pgpd-OsTIR1(F74G)

Plasmid

#247617

Purpose

Expression of OsTIR1

Depositor

Sebastian Schuck

Article

Hubbe et al Mol Biol Cell. 2026 Feb 1;37(2):mr2. doi

Insert

OsTIR1

Expression

Yeast

Mutation

F74G

Promoter

GAP

Available Since

Dec. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pNH605-Padh-AtAFB2(F74A)

Plasmid

#247613

Purpose

Expression of AtAFB2

Depositor

Sebastian Schuck

Article

Hubbe et al Mol Biol Cell. 2026 Feb 1;37(2):mr2. doi

Insert

AtAFB2

Expression

Yeast

Mutation

F74A

Promoter

ADH1

Available Since

Dec. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pNH605-Padh-AtAFB2(F74G)

Plasmid

#247612

Purpose

Expression of AtAFB2

Depositor

Sebastian Schuck

Article

Hubbe et al Mol Biol Cell. 2026 Feb 1;37(2):mr2. doi

Insert

AtAFB2

Expression

Yeast

Mutation

F74G

Promoter

ADH1

Available Since

Dec. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pSC-pGAL10-CRISPRa

Plasmid

#240210

Purpose

Yeast vector for integration of pGAL10-dCas9 and pGAL10-MCP-VPR at LEU2 locus

Depositor

Wilfred Chen

Article

Stohr et al Nucleic Acids Res. 2025 Nov 13;53(21):gk

Inserts

MCP-VPR
dCas9

Expression

Yeast

Promoter

pGAL10

Available Since

Nov. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pDRF1 Cyterm-mGold

Plasmid

#224084

Purpose

Expresses Cyterm-mGold to check for monomericity.

Depositor

Bas Teusink

Article

Botman et al bioRxiv 2025.06.27.661975; doi: https://

Insert

Cyterm-mGold

Expression

Yeast

Available Since

Aug. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pRO275

Plasmid

#233825

Purpose

MoClo-YTK; type3A; Activation domains; NLS-VP48-1XOaf1

Depositor

Jeff Hasty

Article

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

NLS-VP48-1XOaf1

Expression

Yeast

Available Since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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pRO276

Plasmid

#233826

Purpose

MoClo-YTK; type3A; Activation domains; NLS-VP48-2XOaf1

Depositor

Jeff Hasty

Article

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

NLS-VP48-2XOaf1

Expression

Yeast

Available Since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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pWN102

Plasmid

#233828

Purpose

MoClo-YTK; type4A; Activation domains; NLS-VP48-2XOaf1

Depositor

Jeff Hasty

Article

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

NLS-VP48-2XOaf1

Expression

Yeast

Available Since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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pAL002

Plasmid

#233829

Purpose

MoClo-YTK; type4A; Activation domains; VP48-2XOaf1

Depositor

Jeff Hasty

Article

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

VP48-2XOaf1

Expression

Yeast

Available Since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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pRO211

Plasmid

#233830

Purpose

MoClo-YTK; ConLS/RE cassette; Const. mNG; pTDH3-mNeonGreen-tENO2

Depositor

Jeff Hasty

Article

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

pTDH3-mNeonGreen-tENO2

Expression

Yeast

Available Since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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pRO261

Plasmid

#233831

Purpose

MoClo-YTK; ConLS/RE cassette; Const. mNG; pRPL18B-mNeonGreen-tENO2

Depositor

Jeff Hasty

Article

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

pRPL18B-mNeonGreen-tENO2

Expression

Yeast

Available Since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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pRO262

Plasmid

#233832

Purpose

MoClo-YTK; ConLS/RE cassette; Const. mNG; pRNR2-mNeonGreen-tENO2

Depositor

Jeff Hasty

Article

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

pRNR2-mNeonGreen-tENO2

Expression

Yeast

Available Since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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pRO263

Plasmid

#233833

Purpose

MoClo-YTK; ConLS/RE cassette; Const. mNG; pRAD27-mNeonGreen-tENO2

Depositor

Jeff Hasty

Article

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

pRAD27-mNeonGreen-tENO2

Expression

Yeast

Available Since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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pRO264

Plasmid

#233834

Purpose

MoClo-YTK; ConLS/RE cassette; Const. mNG; pREV1-mNeonGreen-tENO2

Depositor

Jeff Hasty

Article

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

pREV1-mNeonGreen-tENO2

Expression

Yeast

Available Since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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pRO227

Plasmid

#233835

Purpose

MoClo-YTK; ConL1/RE cassette; rtTA systems; tetO7-pLEU2m-mNeonGreen-tENO2

Depositor

Jeff Hasty

Article

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

tetO7-pLEU2m-mNeonGreen-tENO2

Expression

Yeast

Available Since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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pRO229

Plasmid

#233836

Purpose

MoClo-YTK; ConLS/R1 cassette; rtTA systems; pRPL18B-rtTA-v10-tADH1

Depositor

Jeff Hasty

Article

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

pRPL18B-rtTA-v10-tADH1

Expression

Yeast

Available Since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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