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ClhN-pADH3-Tgl3-4V5-APEX2-URA

Plasmid

#207066

Purpose

Expressing Tgl3-4V5-APEX2. Marker for lipid droplets.

Depositor

Zhiping Xie

Publication

Li et al mSphere. 2022 Aug 31;7(4):e0010722. doi:

Insert

Tgl3

Tags

4xV5-APEX2

Expression

Yeast

Promoter

pADH3

Available since

March 4, 2024

Availability

Academic Institutions and Nonprofits only

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pNLF1-C_CCNE1:1-410

Plasmid

#211023

Purpose

Mammalian expression of full-length human CCNE1. C-terminal NanoLuc luciferase tag.

Depositor

Cheryl Arrowsmith

Publication

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

CCNE1:M1-A410 (CCNE1 Human)

Tags

NanoLuc luciferase

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pNLF1-C_RBBP7:1-425

Plasmid

#211021

Purpose

Mammalian expression of full-length human RBBP7. C-terminal NanoLuc luciferase tag.

Depositor

Cheryl Arrowsmith

Publication

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

RBBP7:M1-S425 (RBBP7 Human)

Tags

NanoLuc luciferase

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pNLF1-N_DDA1:1-102

Plasmid

#210960

Purpose

Mammalian expression of full-length human DDA1. N-terminal NanoLuc luciferase tag.

Depositor

Cheryl Arrowsmith

Publication

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

DDA1:M1-T102 (DDA1 Human)

Tags

NanoLuc luciferase

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pNLF1-N_RFWD3:1-774

Plasmid

#210954

Purpose

Mammalian expression of full-length human RFWD3. N-terminal NanoLuc luciferase tag.

Depositor

Cheryl Arrowsmith

Publication

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

RFWD3:M1-E774 (RFWD3 Human)

Tags

NanoLuc luciferase

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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EGFP Rab11a WT pcDNA3.1 Zeo (+)

Plasmid

#206079

Purpose

Rab11a belongs to the Rab family of small GTPases, Rab11 proteins are particularly involved in trafficking through recycling endosomes

Depositor

Annette Dolphin

Publication

Tran-Van-Minh et al J Neurosci. 2010 Sep 22;30(38):12856-67.

Insert

RAB11A (RAB11A Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available since

Oct. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-Puro G3BP1-sgRNA

Plasmid

#196197

Purpose

Cas9 + gRNA expression to edit G3BP1 locus

Depositor

Jürgen Knoblich

Publication

Sidhaye et al Elife. 2023 Mar 29;12:e85135. doi: 10.75

Insert

G3BP1 (G3BP1 Human)

Use

CRISPR

Available since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pSEM387 - mosTI unc-119 - NNN - gfp - nls - tbb-2

Plasmid

#182347

Purpose

GFP transcriptional reporter cloning vector for mosTI(unc-119). Transgene inserted upstream of gfp(2xNLS) and tbb-2 3' UTR. MCS or Golden Gate (BsaI: tgcc - MCS - aaaa)

Depositor

Christian Froekjaer-Jensen

Publication

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Type

Empty backbone

Expression

Worm

Available since

May 16, 2022

Availability

Academic Institutions and Nonprofits only

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pSEM398 - mosTI unc-119 - NNN - operon - mScarlet - tbb-2

Plasmid

#182355

Purpose

mScarlet co-expression cloning vector for mosTI(unc-119). Transgene inserted upstream of gpd-2 operon::mScarlet::tbb-2 3'UTR. MCS or Golden Gate (BsaI: tgcc - MCS - tgag)

Depositor

Christian Froekjaer-Jensen

Publication

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Type

Empty backbone

Expression

Worm

Available since

May 13, 2022

Availability

Academic Institutions and Nonprofits only

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pHH0103_NR1I2_Hormone-recep

Plasmid

#110020

Purpose

Protein expression and purification of NR1I2_Hormone-recep

Depositor

Sachdev Sidhu

Publication

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

NR1I2_Hormone-recep (NR1I2 Human)

Expression

Bacterial

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_NR5A2_Hormone-recep

Plasmid

#109818

Purpose

Protein expression and purification of NR5A2_Hormone-recep

Depositor

Sachdev Sidhu

Publication

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

NR5A2_Hormone-recep (NR5A2 Human)

Expression

Bacterial

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pLaNGo11-1

Plasmid

#126053

Purpose

A self-assembling split-GFP based Golden Gate vector to determine protein targeting to plastids in plant cell

Depositor

Ralf-Bernd Klösgen

Publication

Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

Inserts

chloroplastic FNR transit peptide
ccdB cassette (BsaI)
GFP11

Use

Binary

Tags

GFP1-10

Expression

Plant

Available since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-V5-Vrk2

Plasmid

#120240

Purpose

Expresses V5-tagged Vrk2 in lentiviral vector

Depositor

Larry Gerace

Publication

Cheng et al Nucleus. 2019 Dec;10(1):126-143. doi: 10

Insert

Vrk2 (Vrk2 Mouse)

Use

Lentiviral

Tags

V5

Promoter

EF1a

Available since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pCRII-Sema4c-ISH-2kb

Plasmid

#68031

Purpose

Contains 2kb cDNA fragment for probe synthesis for mRNA in situ hybridization, species: mouse

Depositor

Roland Friedel

Publication

Maier et al Mol Cell Neurosci. 2011 Feb;46(2):419-31

Insert

Semaphorin-4C (Sema4c Mouse)

Use

Mrna in situ hybridization probe

Available since

Aug. 27, 2015

Availability

Academic Institutions and Nonprofits only

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GPKAnls

Plasmid

#61094

Purpose

GFP-tagged PKA catalytic subunit targeted to the nucleus by addition of a nuclear localization signal

Depositor

Steven Green

Publication

Bok et al J Neurosci. 2003 Feb 1;23(3):777-87.

Insert

pkac2

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only
mCherry-miniSOG-C-Src-7

Plasmid

#55086

Purpose

Localization: Membrane/Vesicles, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Publication

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

C-Src

Tags

mCherry-miniSOG

Expression

Mammalian

Promoter

CMV

Available since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only
EPHA2 (3MBW)

Plasmid

#25357

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

epha2.023.326 (EPHA2 Human)

Tags

His

Expression

Insect

Available since

Aug. 19, 2010

Availability

Industry, Academic Institutions, and Nonprofits
Keratin 8 S23A

Plasmid

#18057

Depositor

M Bishr Omary

Publication

Ku et al J Biol Chem. 2002 Mar 29. 277(13):10775-

Insert

Keratin 8 (KRT8 Human)

Expression

Mammalian

Mutation

S23A

Available since

May 16, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pCDNA5/FRT/TO_TOM20-dHaloTag7_T2A_Lifeact-HaloTag7

Plasmid

#187076

Purpose

Co-expression of dHaloTag7 at the mitochondria surface and HaloTag7 on actin filaments

Depositor

Kai Johnsson

Publication

Kompa et al J Am Chem Soc. 2023 Feb 8;145(5):3075-30

Insert

TOM20-dHaloTag7_T2A_Lifeact-HaloTag7

Expression

Mammalian

Mutation

dHaloTag7 = HaloTag7-D106A

Available since

Aug. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pFUW PRKN-P2A-PuroR

Plasmid

#239571

Purpose

Overexpression of human PARK2

Depositor

Tim Ahfeldt, Joel Blanchard

Publication

et al iScience 28 (2025)

Insert

PRKN (PRKN Human)

Use

Lentiviral

Promoter

UbC

Available since

June 30, 2025

Availability

Academic Institutions and Nonprofits only

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