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pET28a-FKBP-IRSp53(pLxIS)

Plasmid

#221336

Purpose

Overexpression of IRSp53 pLxIS domain in bacteria

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

IRSp53 pLxIS domain (Baiap2 Mouse)

Tags

6xHis-4xFKBP

Expression

Bacterial

Promoter

T7

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pMSCV-FKBP-DZIP1L

Plasmid

#221290

Purpose

Expression of DZIP1L (pLxIS) in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

DZIP1L (pLxIS) (Dzip1l Mouse)

Use

Retroviral

Tags

FLAG-4xFKBP

Available Since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pMSCV-FKBP-TRIF(pLxIS) S209A

Plasmid

#221245

Purpose

Expression of mutant TRIF pLxIS domain (1-222) in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

TRIF pLxIS domain (1-222) (Ticam1 Mouse)

Use

Retroviral

Tags

3xFLAG-4xFKBP

Mutation

S209A

Available Since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pMSCV-FKBP-TRIF(pLxIS) L193A

Plasmid

#221246

Purpose

Expression of mutant TRIF pLxIS domain (1-222) in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

TRIF pLxIS domain (1-222) (Ticam1 Mouse)

Use

Retroviral

Tags

3xFLAG-4xFKBP

Mutation

L193A

Available Since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-IRAK4-mScarlet-P2A-Puro

Plasmid

#221237

Purpose

Stable expression of mouse IRAK4-mScarlet after Lentiviral transduction

Depositor

Jonathan Kagan

Article

Fisch et al Nature. 2024 Jul;631(8021):635-644. doi:

Insert

IRAK4 (Irak4 Mouse)

Use

Lentiviral

Tags

mScarlet

Available Since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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NSP4-Δ220-234-Flag

Plasmid

#210341

Purpose

Expresses SARS-CoV-2 NSP4-Δ220-234 fused with Flag in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

SARS-CoV-2 NSP4 (ORF1ab Human, SARS-CoV-2 isolate Wuhan-Hu-1)

Tags

Flag

Expression

Mammalian

Mutation

Δ220-234

Available Since

Jan. 3, 2024

Availability

Academic Institutions and Nonprofits only

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NSP4-H120N/F121L-Flag

Plasmid

#210340

Purpose

Expresses SARS-CoV-2 NSP4-H120N/F121L fused with Flag in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

SARS-CoV-2 NSP4 (ORF1ab Human, SARS-CoV-2 isolate Wuhan-Hu-1)

Tags

Flag

Expression

Mammalian

Mutation

H120N/F121L

Available Since

Jan. 3, 2024

Availability

Academic Institutions and Nonprofits only

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NSP4-FR-2Strep

Plasmid

#210316

Purpose

Expresses SARS-CoV-2 NSP4 in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

SARS-CoV-2 NSP4 (ORF1ab Human, SARS-CoV-2 isolate Wuhan-Hu-1)

Tags

2Strep

Expression

Mammalian

Mutation

Many synonymous changes due to codon optimization

Available Since

Jan. 3, 2024

Availability

Academic Institutions and Nonprofits only

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NSP4-mCherry

Plasmid

#210319

Purpose

Expresses SARS-CoV-2 NSP4 fused with mCherry in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

SARS-CoV-2 NSP4 (ORF1ab Human, SARS-CoV-2 isolate Wuhan-Hu-1)

Tags

mCherry

Expression

Mammalian

Mutation

Many synonymous changes due to codon optimization

Available Since

Jan. 3, 2024

Availability

Academic Institutions and Nonprofits only

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STIM1-2Strep

Plasmid

#210361

Purpose

Expresses STIM1 fused with 2Strep in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

STIM1 (STIM1 Human)

Tags

2Strep

Expression

Mammalian

Available Since

Dec. 18, 2023

Availability

Academic Institutions and Nonprofits only

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FKBP10-2Strep

Plasmid

#210357

Purpose

Expresses FKBP10 fused with 2Strep in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

FKBP10 (FKBP10 Human)

Tags

2Strep

Expression

Mammalian

Available Since

Dec. 18, 2023

Availability

Academic Institutions and Nonprofits only

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DNAJC11-2Strep

Plasmid

#210354

Purpose

Expresses DNAJC11 fused with 2Strep in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

DNAJC11 (DNAJC11 Human)

Tags

2Strep

Expression

Mammalian

Available Since

Dec. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pNMSB96

Plasmid

#199316

Purpose

MosSCI insertion of flp-18p::lox2272::mTagBFP2::tbb-2 3'UTR::lox2272::ACR1::SL2::jRGECO1a::let-858 3'UTR at ttTi5605

Depositor

Michael Krieg

Article

Porta-de-la-Riva et al Nat Methods. 2023 Apr 6. doi: 10.1038/s4

Insert

flp-18p::lox2272::mTagBFP2::tbb-2 3'UTR::lox2272::ACR1::SL2::jRGECO1a::let-858 3'UTR

Expression

Worm

Mutation

mTagBFP2 from pJJR81, C. elegans codon optimized …

Promoter

flp-18

Available Since

Aug. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pRetroX-TRE3G_NT-GSDMD_I105N__C192A

Plasmid

#201176

Purpose

Dox-inducible expression of NT-GSDMD gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Devant et al Cell Rep. 2023 Jan 31;42(1):112008. doi:

Insert

Gasdermin D N-terminal domain (Gsdmd Mouse)

Use

Retroviral

Mutation

aa1-276 only, I105N, C192A

Promoter

TRE3G

Available Since

June 6, 2023

Availability

Academic Institutions and Nonprofits only

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pRetroX-TRE3G_NT-GSDMD_C192A

Plasmid

#201172

Purpose

Dox-inducible expression of NT-GSDMD gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Devant et al Cell Rep. 2023 Jan 31;42(1):112008. doi:

Insert

Gasdermin D N-terminal domain (Gsdmd Mouse)

Use

Retroviral

Mutation

aa1-276 only, C192A

Promoter

TRE3G

Available Since

June 6, 2023

Availability

Academic Institutions and Nonprofits only

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pRetroX-TRE3G_NT-GSDMD_I105N_C39A

Plasmid

#201175

Purpose

Dox-inducible expression of NT-GSDMD gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Devant et al Cell Rep. 2023 Jan 31;42(1):112008. doi:

Insert

Gasdermin D N-terminal domain (Gsdmd Mouse)

Use

Retroviral

Mutation

aa1-276 only, I105N, C39A

Promoter

TRE3G

Available Since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

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pRetroX-TRE3G_NT-GSDMD_C39A

Plasmid

#201171

Purpose

Dox-inducible expression of NT-GSDMD gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Devant et al Cell Rep. 2023 Jan 31;42(1):112008. doi:

Insert

Gasdermin D N-terminal domain (Gsdmd Mouse)

Use

Retroviral

Mutation

aa1-276 only, C39A

Promoter

TRE3G

Available Since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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pS238D1::sptse5-CT

Plasmid

#192959

Purpose

For expression of Tse5-CT (1169-1317) in P. putida with the PelB signal peptide fused at the N-terminus in biological function studies

Depositor

David Albesa-Jové

Article

Gonzalez-Magana et al Commun Biol. 2022 Nov 5;5(1):1189. doi:

Insert

sptse5-CT

Tags

PelB signal peptide

Expression

Bacterial

Mutation

encodes for tse5 (1169-1317)

Available Since

Dec. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pMSCV-IRES-GFP-human-caspase-1/4a

Plasmid

#183393

Purpose

Expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

Insert

CARD domain of human caspase-1 fused to catalytic domain of human caspase-4 (CASP1 Synthetic, Human)

Use

Retroviral

Tags

Myc

Promoter

MESV

Available Since

Aug. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2-zfKitlga

Plasmid

#168199

Purpose

Express zebrafish Kitlga, mRNA synthesis

Depositor

Petr Bartunek

Article

Oltova et al Blood Adv. 2020 Dec 8;4(23):5915-5924. d

Insert

Kitlga (kitlga Zebrafish)

Use

Vertebrate expression (zebrafish, chicken, xenopu…

Expression

Mammalian

Mutation

F198L - please see depositor comments

Available Since

Nov. 3, 2021

Availability

Academic Institutions and Nonprofits only

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