We narrowed to 4,107 results for: biorxiv

1. Anti-pTau nanobody (A2) with sortase tag

   Plasmid

   #233218

   Purpose

   Mammalian epression of anti-pTau nanobody (A2) with a sortase tag for direct dye conjugation.

   Depositor

   Jeff Lichtman

   Article

   Han et al bioRxiv 2023.10.24.563674

   Insert

   Anti-pTau nanobody (A2)
   

   Tags

   Sortase tag, His tag

   Expression

   Mammalian

   Available Since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. 0_T7-mScarlet3

   Plasmid

   #225927

   Purpose

   In vitro synthesis of mRNA for fluorescent protein mScarlet3 (control)

   Depositor

   Michalis Averof

   Article

   Karapidaki et al bioRxiv 2024.11.12.623055

   Insert

   mScarlet3
   

   Use

   In vitro transcription

   Promoter

   T7

   Available Since

   Nov. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHAGE2-EF1a-E1MLS-2xFLAG-LigD-IRES-tdTomato

   Plasmid

   #234951

   Purpose

   Human codon optimized LigD (Mycobacterium) with N-terminal E1 MLS expressing plasmid

   Depositor

   Agnel Sfeir

   Article

   Fu et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

   Insert

   Human codon optimized LigD with N-terminal mitochondrial localization signal of pyruvate dehydrogenase E1 subunit
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   EF1A

   Available Since

   April 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG-eSpCas9-2A-GFP-sgRNA-DNA-PKcs

   Plasmid

   #220493

   Purpose

   To generate DNA-PKcs KO in human cells. Co-expresses eSpCas9(1.1), GFP and a guide RNA against DNA-PKcs exon 1.

   Depositor

   Sébastien Britton

   Article

   Bossaert et al bioRxiv [Preprint]. 2024 Jan 4:2024.01.0

   Insert

   sgRNA targeting DNA-PKcs (PRKDC) exon 1 (PRKDC Human)
   

   Use

   CRISPR

   Promoter

   U6

   Available Since

   July 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. lentiTet-LDLR-mCherry BlastR

   Plasmid

   #186739

   Purpose

   Dox inducible LDLR coding sequence expression construct cloned into a lentiviral backbone containing a blasticidin resistance gene

   Depositor

   Richard Sherwood

   Article

   Hamilton et al bioRxiv. 2023 Jan 10:2023.01.09.500804.

   Insert

   LDLR (LDLR Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   April 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBK2044-AAV-2xSp1-2xNFkB-EFSNC-dSaCas9-KRAB-MECP2

   Plasmid

   #223164

   Purpose

   2nd gen. vector for expression of KRAB and truncated MECP2 with dSaCas9 and empty gRNA scaffold

   Depositor

   Boris Kantor

   Article

   Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

   Insert

   KRAB-MECP2 (MECP2 Human)
   

   Use

   AAV and CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Encodes aa 204-310 (MECP2)

   Promoter

   EF1a

   Available Since

   Aug. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBK1304-AAV-EFSNC-dCjCas9-KRAB-MECP2

   Plasmid

   #223149

   Purpose

   Expression of KRAB and truncated MECP2 with dCjCas9 and empty gRNA scaffold

   Depositor

   Boris Kantor

   Article

   Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

   Insert

   KRAB-MECP2 (MECP2 Human)
   

   Use

   AAV and CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Encodes aa 204-310 (MECP2)

   Promoter

   EF1a

   Available Since

   Aug. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBOB-CAG-SARS-CoV2-Spike-HA

   Plasmid

   #141347

   Purpose

   3rd Generation lentiviral vector expressing the codon-optimized SARS-CoV2 Spike Glycoprotein ORF with a C-terminal HA tag generated by Junko Ogawa & Gerald M Pao

   Depositor

   Gerald Pao

   Article

   Ogawa et al bioRxiv. 2020 Jul 22. doi: 10.1101/2020.

   Insert

   SARS-CoV2 Spike Glycoprotein (S SARS-CoV2 hCoV19_USA EPI_ISL_414366 (GISAID))
   

   Use

   Lentiviral

   Tags

   HA

   Mutation

   resynthesized with human codon optimization nucle…

   Promoter

   CAG

   Available Since

   May 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCF142_U6-sgRNA

   Plasmid

   #225960

   Purpose

   U6-sgRNA (recipient). Expresses U6-sgRNA (recipient) for VLP production. This is a recipient vector for cloning of specific SpyCas9 sgRNAs.

   Depositor

   Christof Fellmann

   Article

   Ramadoss et al bioRxiv [Preprint]. 2024 Jun 26:2024.06.

   Insert

   U6-sgRNA (recipient)
   

   Expression

   Mammalian

   Available Since

   Oct. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCF153_Gag-Pol

    Plasmid

    #225961

    Purpose

    CMV-Intron-GagPol (FMLV). Expresses FMLV (Friend Murine Leukemia Virus) Gag-Pol for VLP production.

    Depositor

    Christof Fellmann

    Article

    Ramadoss et al bioRxiv [Preprint]. 2024 Jun 26:2024.06.

    Insert

    CMV-Intron-GagPol (FMLV)
    

    Expression

    Mammalian

    Available Since

    Oct. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCF140_Gag-SpyCas9-NLS

    Plasmid

    #225959

    Purpose

    CMV-Intron-Gag-SpyCas9-NLS. Expresses Gag-SpyCas9-NLS for VLP production.

    Depositor

    Christof Fellmann

    Article

    Ramadoss et al bioRxiv [Preprint]. 2024 Jun 26:2024.06.

    Insert

    CMV-Intron-Gag-SpyCas9-NLS
    

    Expression

    Mammalian

    Available Since

    Oct. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pZac2.1-gfaABC1D-PinkyCaMP

    Plasmid

    #232859

    Purpose

    An AAV vector expressing the mScarlet based calcium indicator PinkyCaMP under gfaABC1D promoter

    Depositor

    Olivia Masseck

    Article

    Fink et al bioRxiv [Preprint]. 2025 Jan 11:2024.12.

    Insert

    PinkyCaMP
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    gfaABC1D

    Available Since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. 4_T7-mScarlet3-RitC

    Plasmid

    #225931

    Purpose

    In vitro synthesis of mRNA for fluorescent protein mScarlet3 tagged with the H-RitC membrane localisation signal

    Depositor

    Michalis Averof

    Article

    Karapidaki et al bioRxiv 2024.11.12.623055

    Insert

    mScarlet3-RitC
    

    Use

    In vitro transcription

    Promoter

    T7

    Available Since

    Nov. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAGEN-Vhh-LaminB-mNeonGreen-IRES-myr-BFP (JDW 1353)

    Plasmid

    #229845

    Purpose

    A CAGGS-driven expression vector containing a vhh-Lamin nanobody for nuclear lamina labeling followed by an IRES and a myristoylated TagBFP for labeling the cell membrane.

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    Lamin nanobody
    

    Expression

    Mammalian

    Available Since

    Feb. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHE111-3xFlag-HSP70

    Plasmid

    #236055

    Purpose

    Mammalian expression of human HSP70 with a N-terminal 3x-FLAG tag

    Depositor

    Jonathan Schisler

    Article

    Stewart et al bioRxiv 2025.02.17.637997

    Insert

    HSP70 (HSPA1A Human)
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available Since

    Aug. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBK1314-AAV-EFSNC-dSaCas9-KRAB-MECP2

    Plasmid

    #223159

    Purpose

    Expression of KRAB and truncated MECP2 with dSaCas9 and empty gRNA scaffold

    Depositor

    Boris Kantor

    Article

    Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

    Insert

    KRAB-MECP2 (MECP2 Human)
    

    Use

    AAV and CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Encodes aa 204-310 (MECP2)

    Promoter

    EF1a

    Available Since

    Aug. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pStreptagIIx2 Alfatag 3C InsR EABR

    Plasmid

    #234996

    Purpose

    For production of Extracellular Vesicles (EVs), with the Insulin Receptor Strep-tag II labeled on their surface

    Depositor

    Monica Gonzalez-Magaldi, Daniel Leahy

    Article

    Gonzalez-Magaldi et al bioRxiv [Preprint]. 2024 Nov 28:2024.11.

    Insert

    InsR-EABR (INSR Human)
    

    Tags

    2xStreptag-II, Alfatag, HRV 3C site

    Expression

    Mammalian

    Available Since

    April 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHA TST 3C SMO EABR

    Plasmid

    #234989

    Purpose

    For production of Extracellular Vesicles (EVs), with the receptor Smoothened (Smo), Twin-Strep-tag (TST), and an HA tag at its N-terminus on their surface

    Depositor

    Monica Gonzalez-Magaldi, Daniel Leahy

    Article

    Gonzalez-Magaldi et al bioRxiv [Preprint]. 2024 Nov 28:2024.11.

    Insert

    SMO-EABR (SMO Human)
    

    Tags

    HA, Twin-Strep-Tag, HRV 3C site

    Expression

    Mammalian

    Available Since

    April 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pStreptagII 3C EGFR EABR

    Plasmid

    #234994

    Purpose

    For production of Extracellular Vesicles (EVs), with Epithelial Growth Factor Receptor on their surface, Strep-tag II labeled on its N-terminus

    Depositor

    Monica Gonzalez-Magaldi, Daniel Leahy

    Article

    Gonzalez-Magaldi et al bioRxiv [Preprint]. 2024 Nov 28:2024.11.

    Insert

    EGFR-EABR (EGFR Human)
    

    Tags

    Streptag-II, HRV 3C site

    Expression

    Mammalian

    Available Since

    April 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHA StreptagII 3C NIS EABR

    Plasmid

    #234988

    Purpose

    For production of Extracellular Vesicles (EVs), with a Strep-tag II labeled Sodium-iodide symporter (NIS), and an HA epitope at its N-terminus on their surface

    Depositor

    Monica Gonzalez-Magaldi, Daniel Leahy

    Article

    Gonzalez-Magaldi et al bioRxiv [Preprint]. 2024 Nov 28:2024.11.

    Insert

    NIS-EABR (SLC5A5 Human)
    

    Tags

    HA, Streptag-II, HRV 3C site

    Expression

    Mammalian

    Available Since

    March 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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