We narrowed to 3,830 results for: Gateway entry

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pEN_TRmiRc2

Plasmid

#25750

Purpose

Entry vector for cloning miR30-based shRNA driven by TRE promoter with dsRed2 coexpression.

Depositor

Iain Fraser

Publication

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Type

Empty backbone

Use

Gateway Cloning

Tags

dsRed2

Available since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pJG85

Plasmid

#89281

Purpose

Entry vector containing the TaU6 promoter and an Esp3I Golden Gate cloning site for cloning of the sgRNA, all between attL5 and attL2 sites

Depositor

Daniel Voytas

Publication

Gil-Humanes et al Plant J. 2016 Dec 10. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available since

May 1, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pYZ673

Plasmid

#132953

Purpose

crRNA entry vector of Pfba1-BsaI pad-TEF1 Term with FnCas12a in Schizosaccharomyces pombe

Depositor

Jef Boeke

Publication

Zhao et al Nucleic Acids Res. 2020 Jun 4;48(10):578

Insert

FnCas12a

Use

CRISPR and Synthetic Biology

Promoter

adh1

Available since

April 30, 2021

Availability

Academic Institutions and Nonprofits only

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p3E-Dusp6DM_S159/197A

Plasmid

#67684

Purpose

3' entry clone with activated Dusp6 (S159/167A)

Depositor

Nathan Lawson

Publication

Shin et al Development. 2016 Oct 15;143(20):3796-38

Insert

dusp6 (dusp6 Zebrafish)

Mutation

S159A, S197A

Available since

Aug. 7, 2015

Availability

Academic Institutions and Nonprofits only

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pME-6MTzactMap2k2b

Plasmid

#67710

Purpose

Middle entry clone

Depositor

Nathan Lawson

Publication

Shin et al Development. 2016 Oct 15;143(20):3785-37

Insert

activated Map2k2b (map2k2b Zebrafish)

Mutation

S221D, S225D

Promoter

none

Available since

Aug. 7, 2015

Availability

Academic Institutions and Nonprofits only

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pME-6MTnlstdTomato2A

Plasmid

#67685

Purpose

Middle entry nuclear localized tdTomato reporter with 6xMT and 2A

Depositor

Nathan Lawson

Publication

Shin et al Development. 2016 Oct 15;143(20):3796-38

Insert

tdTomato

Tags

2A and 6xMyc-NLS

Available since

Aug. 6, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLG371

Plasmid

#128410

Purpose

Cuminic acid-inducible system

Depositor

Christopher Voigt

Publication

Gonzalez et al Nat Chem Biol. 2019 Dec 2. pii: 10.1038/

Insert

gfpmut2 (NEWENTRY Synthetic)

Expression

Bacterial

Promoter

Pspank(C)

Available since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pENTR221-APP751

Plasmid

#193772

Purpose

Entry vector for human APP isoform having 751 amino acid residues

Depositor

Wade Harper

Publication

Park et al Nat Commun. 2022 Oct 16;13(1):6112. doi:

Insert

APP (APP Human)

Available since

Dec. 16, 2022

Availability

Academic Institutions and Nonprofits only

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KJ901: pMAGIC (R4-R3) NLS-(SrfI/PmeI)-NLS

Plasmid

#121835

Purpose

pMAGIC R4-R3 entry plasmid, contains 2x NLS scaffold enabling addition of dCas9 mutants into pMAGIC. dCas9 can be inserted via unique SrfI/PmeI restriction sites

Depositor

Christopher Newgard

Publication

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Gateway Cloning and Synthetic Biology

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pEN_hU6miR-Luc-A

Plasmid

#25756

Purpose

Entry vector with human U6 promoter driving control Luciferase miR30-based shRNA.

Depositor

Iain Fraser

Publication

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Insert

Luciferase miR-shRNA

Use

Gateway Cloning

Available since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

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pYZ714

Plasmid

#132955

Purpose

crRNA entry vector of Pfba1-BsaI pad-TEF1 Term with dFnCas12a-Clr4 in Schizosaccharomyces pombe

Depositor

Jef Boeke

Publication

Zhao et al Nucleic Acids Res. 2020 Jun 4;48(10):578

Insert

dFnCas12a

Use

CRISPR and Synthetic Biology

Tags

SV40 NLS and nucleoplasmin NLS

Promoter

adh1

Available since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pENTR_TiTRE

Plasmid

#172128

Purpose

Entry vector for tightly controlled gene expression by a tetracycline-responsive promoter

Depositor

Kenji Kontani

Publication

Araki et al J Biol Chem. 2021 Nov 18:101428. doi: 10

Type

Empty backbone

Use

Gateway Cloning

Expression

Mammalian

Promoter

tight TRE

Available since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pEN_TGmiR_Gb2-K

Plasmid

#25766

Purpose

Entry vector withTRE promoter driving mouse G beta 2 miR30-based shRNA with GFP coexpression.

Depositor

Iain Fraser

Publication

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Insert

Gb2 miR-shRNA (Gnb2 Mouse)

Use

Gateway Cloning

Tags

GFP

Available since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

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dpYPQ230-D156R-ABE4

Plasmid

#195370

Purpose

dLbCas12a-D156R based adenine base editor, ecTadA8e fused to the N terminal of dLbCas12a-D156R with the linker of (GGGGS)x6

Depositor

Yiping Qi

Publication

Cheng et al Plant Commun. 2023 Apr 13:100601. doi: 1

Insert

ecTadA8e-(GGGGS)x6-dLbCas12a-D156R

Use

CRISPR; Gateway compatible ectada8e-(ggggs)x6-dlb…

Tags

5' and 3' NLS

Expression

Plant

Promoter

ZmUBI1

Available since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

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p5E-pax3a-5.4k

Plasmid

#164966

Purpose

zebrafish pax3a -5.4k promoter

Depositor

GuangJun Zhang

Publication

Silic et al Genetics. 2020 Aug;215(4):1067-1084. doi

Insert

pax3a (pax3a Zebrafish)

Use

Gateway 5 end entry vector

Mutation

promoter -5.4k

Available since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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pZA13

Plasmid

#158491

Purpose

Entry clone for Golden Gate assembly. Golden gate compatible N. benthamiana ZAR1 without STOP codon

Depositor

Sophien Kamoun

Publication

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

ZAR1

Use

Golden gate entry vector

Mutation

no STOP codon

Available since

Sept. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pZA8

Plasmid

#158486

Purpose

Entry clone for Golden Gate assembly. Golden gate compatible N. benthamiana ZAR1 L17E/D481V with STOP codon

Depositor

Sophien Kamoun

Publication

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

ZAR1

Use

Golden gate entry vector

Mutation

L17E, D481V, STOP codon

Available since

Sept. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pFloat.T7_v1

Plasmid

#133070

Purpose

Entry vector for cloning genes under a T7 promoter for expression in bacteria. Version 1.0.

Depositor

Kacper Rogala, David Sabatini

Publication

Rogala et al Science. 2019 Oct 25;366(6464):468-475.

Type

Empty backbone

Use

Gateway Cloning

Tags

3C-His6 and His6-3C

Expression

Bacterial

Promoter

T7

Available since

May 26, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR223-SLC7A11 sh926R

Plasmid

#118701

Purpose

entry clone for SLC7A11 insensitive to hairpin TRCN0000288926

Depositor

William Kaelin

Publication

Briggs et al Cell. 2016 Jun 30;166(1):126-39. doi: 10

Insert

SLC7A11 (SLC7A11 Human)

Expression

Mammalian

Mutation

wt , SLC7A11 sh926R

Available since

Nov. 8, 2018

Availability

Academic Institutions and Nonprofits only

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dpYPQ230-D156R-CBE1

Plasmid

#195367

Purpose

dLbCas12a-D156R based cytosine base editor, hA3A/Y130F fused to the N terminal of dLbCas12a-D156R with the linker of XTEN with 3xFLAG

Depositor

Yiping Qi

Publication

Cheng et al Plant Commun. 2023 Apr 13:100601. doi: 1

Insert

hA3A/Y130F-XTEN with 3xFLAG-dLbCas12a-D156R-UGI

Use

CRISPR; Gateway compatible ha3a/y130f-xten plus 3…

Tags

3xflag

Expression

Plant

Promoter

ZmUBI1

Available since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

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