CreLite: An optogenetically controlled Cre/loxP system using red light.
Yen ST, Trimmer KA, Aboul-Fettouh N, Mullen RD, Culver JC, Dickinson ME, Behringer RR, Eisenhoffer GT
Dev Dyn. 2020 Nov;249(11):1394-1403. doi: 10.1002/dvdy.232. Epub 2020 Aug 31.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 131780 | pCS2+PIF6CreN | Mammalian, Zebrafish, or Xenopus expression of PIF6CreN, one half of the CreLite system |
| 131781 | pCS2+PhyBΔCreC | Mammalian, Zebrafish, or Xenopus expression of PhyBΔCreC, one half of the CreLite system. |
| 131782 | pME-CreLite | Middle entry vector (Tol2 kit) with bi-cistronic CreLite system components, PhyBΔCreC and PIF6CreN, linked by P2A. |
| 131783 | pTol2-CreLite | Tol2 destination vector carrying Ubi:CreLite with mTagBFP2 as a visual marker. |
| 131785 | pAAV-CreLite | AAV donor/transfer vector expressing CreLite system components, PhyBΔCreC and PIF6CreN, driven by CBh promoter |
| 131786 | pLenti-CreLite | Lentiviral vector expressing CreLite, driven by CBh promoter |
| 131787 | pTol2-PcyA-IRES-HO1 | Tol2 bicistronic vector expressing PcyA and HO1 for Phycocyanobilin (PCB) synthesis, driven by heatshock promoter hsp70. |