PurposeMammalian, Zebrafish, or Xenopus expression of PIF6CreN, one half of the CreLite system
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||131780||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4095
- Total vector size (bp) 4685
Vector typeMammalian Expression, Cre/Lox ; Zebrafish expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesA. thaliana (mustard weed), Synthetic; Bacteriophage P1
Insert Size (bp)603
GenBank ID825382 2777477
- Promoter CMV
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer SP6
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Read the preprint at bioRxiv: doi.org/10.1101/823971.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCS2+PIF6CreN was a gift from George Eisenhoffer (Addgene plasmid # 131780 ; http://n2t.net/addgene:131780 ; RRID:Addgene_131780)
For your References section:CreLite: An optogenetically controlled Cre/loxP system using red light. Yen ST, Trimmer KA, Aboul-Fettouh N, Mullen RD, Culver JC, Dickinson ME, Behringer RR, Eisenhoffer GT. Dev Dyn. 2020 Nov;249(11):1394-1403. doi: 10.1002/dvdy.232. Epub 2020 Aug 31. 10.1002/dvdy.232 PubMed 32745301