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We narrowed to 9,837 results for: pET

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Showing: 3741 - 3760 of 9837 results

  4. pCJ210

    Plasmid
    #162683
    Purpose
    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating S136C, G489C, S530C, and a sequence Tyr75:Phe104 to introduce a 6th and 7th disulfide bond as in AoFaeB-2.
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1)
    Tags
    His
    Expression
    Bacterial
    Mutation
    S136C, G489C, S530C, and a sequence Y75-F104 to i…
    Promoter
    T7/lac
    Available Since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    162683-plasmid-map-sequence-id-321486

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  9. pET28a-UBC9-K14R-A129K

    Plasmid
    #228948
    Purpose
    express a fragment of the double mutant of the human UBC9 (generated from pET28-UBC9 through mutagenesis; point mutations: K14R and A129K)
    Depositor
    Marcin Suskiewicz
    Insert
    UBC9 (UBE2I Human)
    Tags
    His tag and thrombine cleavage site : MGHHHHHHSSG…
    Expression
    Bacterial
    Mutation
    changed Lysine 14 to Arginine, changed Alanine 12…
    Promoter
    T7-lac
    Available Since
    May 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    228948-plasmid-map-sequence-id-472389

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  18. pET28a-SnoopTag-SpyTag-(AffiHER2)x3

    Plasmid
    #216281
    Purpose
    Three affibodies to HER2 linked in a chain for bacterial expression. SnoopTag and SpyTag allow isopeptide bond formation to their cognate Catcher
    Depositor
    Mark Howarth
    Article
    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:
    Insert
    SnoopTag-SpyTag-(AffiHER2)x3
    Use
    Affinity Reagent/ Antibody
    Tags
    His6, SnoopTag, and SpyTag
    Expression
    Bacterial
    Promoter
    T7 promoter
    Available Since
    June 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    216281-plasmid-map-sequence-id-433204

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  19. pET28-H12TEV-hEIF5B-1~1220(FL)

    Plasmid
    #208636
    Purpose
    for E. coli expression of human eIF5B (ENST00000289371)
    Depositor
    Quanfu Ma
    Insert
    human eIF5B (ENST00000289371) (EIF5B Human)
    Tags
    6xhis
    Expression
    Bacterial
    Mutation
    Contain SNP rs1376443, protein sequence not chang…
    Promoter
    T7
    Available Since
    May 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    208636-plasmid-map-sequence-id-433645

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Showing: 3741 - 3760 of 9837 results