We narrowed to 9,265 results for: CAG

1. pCyno-AAVS1-TetOn-ZIM3-KRAB-dCas9-P2A-mCherry

   Plasmid

   #212830

   Purpose

   Doxycycline-inducible (Zim3)KRAB-dCas9-HA-P2A-mCherry cassette for integration at the AAVS1-locus of the cynomolgus genome.

   Depositor

   Wolfgang Enard

   Article

   Edenhofer et al iScience. 2024 May 23;27(6):110090. doi:

   Insert

   ZIM3-KRAB-dCas9-P2A-mCherry
   

   Use

   Tags

   HA-tag and ZIM3-KRAB

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CAG, TetOn

   Available since

   June 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGorilla-AAVS1-TetOn-ZIM3-KRAB-dCas9-P2A-mCherry

   Plasmid

   #212831

   Purpose

   Doxycycline-inducible (Zim3)KRAB-dCas9-HA-P2A-mCherry cassette for integration at the AAVS1-locus of the gorilla genome.

   Depositor

   Wolfgang Enard

   Article

   Edenhofer et al iScience. 2024 May 23;27(6):110090. doi:

   Insert

   ZIM3-KRAB-dCas9-P2A-mCherry
   

   Use

   Tags

   HA-tag and ZIM3-KRAB

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CAG, TetOn

   Available since

   June 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAV-APOBEC1-YTHmut-EGFP

   Plasmid

   #209323

   Purpose

   AAV packaging vector containing a APOBEC1-YTHmut expression cassette, a P2A-EGFP expression cassette.

   Depositor

   Magdalena Koziol

   Article

   Feng et al Genome Res. 2024 Jul 23;34(6):822-836. d

   Insert

   APOBEC1--YTHmut-HA
   

   Use

   AAV

   Tags

   HA

   Expression

   Mutation

   YTH domain lacks AA 385-409 comprising the m6A-bi…

   Promoter

   Available since

   May 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAV-EGFP-APOBEC1-YTHmut

   Plasmid

   #209320

   Purpose

   AAV packaging vector containing a EGFP-P2A expression cassette, APOBEC1-YTHmut cassette.

   Depositor

   Magdalena Koziol

   Article

   Feng et al Genome Res. 2024 Jul 23;34(6):822-836. d

   Insert

   APOBEC1-YTHmut-HA
   

   Use

   AAV

   Tags

   HA

   Expression

   Mutation

   YTH domain lacks AA 385-409 comprising the m6A-bi…

   Promoter

   Available since

   May 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBVboostFGII WPRE 3C-CVB3

   Plasmid

   #202077

   Purpose

   Bacterial expression of 3C protease from coxsackievirus B3 (CVB3). Contains C-terminal His6-tag.

   Depositor

   Vesa Hytönen

   Article

   Jouppila et al Microbiol Spectr. 2023 Jun 15;11(3):e053

   Insert

   CVB3 3C protease with HisTag
   

   Use

   Tags

   6xHis-tag

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Promoter

   polh, CAG, T7

   Available since

   Aug. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBVboostFGII WPRE 2A-CVB3

   Plasmid

   #202076

   Purpose

   Bacterial expression of 2A protease from coxsackievirus B3 (CVB3). Contains C-terminal His6-tag.

   Depositor

   Vesa Hytönen

   Article

   Jouppila et al Microbiol Spectr. 2023 Jun 15;11(3):e053

   Insert

   CVB3 2A protease with HisTag
   

   Use

   Tags

   6xHis-tag

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Promoter

   polh, CAG, T7

   Available since

   Aug. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPBpuro-EGFP-eDHFR(69K6)-cRaf

   Plasmid

   #178849

   Purpose

   Mammalian expression of cRaf fused to EGFP-eDHFR(69K6)

   Depositor

   Shinya Tsukiji

   Article

   Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

   Insert

   EGFP-eDHFR(69K6)-cRaf (RAF1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   July 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPBpuro-cRaf-mNG-eDHFR(69K6)

   Plasmid

   #172107

   Purpose

   Mammalian expression of cRaf fused to mNeonGreen-eDHFR(69K6)

   Depositor

   Shinya Tsukiji

   Article

   Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

   Insert

   cRaf-mNeonGreen-eDHFR(69K6) (RAF1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   July 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJ175

   Plasmid

   #29493

   Depositor

   Jonathan Tait

   Article

   Jin et al J Biol Chem 2004; 279:40351-57.

   Insert

   annexin V-128 (ANXA5 Human)
   

   Use

   Tags

   (Met)-Ala-Gly-Gly-Cys-Gly-His

   Expression

   Bacterial

   Mutation

   Deletion of the initiator Met at position 1 of wi…

   Promoter

   Available since

   Aug. 22, 2011

   Availability

   Academic Institutions and Nonprofits only

10. pMS77

    Plasmid

    #215681

    Purpose

    Cas9 + guide plasmid targeting ChrIII split hygromycinR landing pad

    Depositor

    Patrick Phillips

    Article

    Moerdyk-Schauwecker et al MicroPubl Biol. 2024 Jan 29;2024:10.1791

    Insert

    eef-1A.1p::Cas9 + U6p::GTCCAGCGGCAGATCGGCGG
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    March 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTC362

    Plasmid

    #91216

    Purpose

    protoplast vector for targeted deletion of 6 genes in tomato

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    gRNAs targeting 6 tomato genes
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    July 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pk335-CARGO-11mer-35kb-DSF-1-11

    Plasmid

    #227489

    Purpose

    11-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus

    Depositor

    Joanna Wysocka

    Article

    Jensen et al Mol Cell. 2025 Jan 16;85(2):347-361.e7.

    Insert

    CARGO to 35kb Downstream Fgf5
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pUDP123

    Plasmid

    #107269

    Purpose

    pUDP123 expressing a polycistronic g-RNA array for Cas9 editing targeting the genes OpADE2 and OpNIAD and Spcas9D147Y P411T in O. parapolymorpha (HH-gRNAOpADE2-HDV-linker-HH-gRNAOpNIAD-HDV)

    Depositor

    Jean-Marc Daran

    Article

    Juergens et al FEMS Yeast Res. 2018 May 1;18(3). pii: 4

    Insert

    polycistronic HH-gRNA-HDV-HH-gRNA-HDV array targetting OpADE2 and NIAD in O. parapolymorpha
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ScTDH3

    Available since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV Syn Barcode22

    Plasmid

    #226193

    Purpose

    Expression mapping

    Depositor

    Scott Sternson

    Insert

    Syn Barcode22
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. SGEP-sh-Hs-PHGDH-1956

    Plasmid

    #188670

    Purpose

    shRNA

    Depositor

    Lewis Cantley

    Insert

    shPHGDH (PHGDH Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    Oct. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pENTRY4_ WRKY28_2

    Plasmid

    #126900

    Purpose

    Gateway pENTR-plasmid to generate a binary construct for genome editing of the wheat homolog of a susceptibility gene of O. sativa. Encodes wheat optimized Cas9 and gene specific sgRNA modules.

    Depositor

    Sophien Kamoun

    Insert

    Wheat_live_Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. OgeuIscB GCA RNA

    Plasmid

    #222864

    Purpose

    This plasmid codes for the guide of the OgeuIscB with a GCA target

    Depositor

    Vincent Dion

    Article

    Mangin et al bioRxiv 2023.10.24.563270

    Insert

    OgeuIscB omega RNA with a (GCA)6 target sequence
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human U6

    Available since

    Oct. 16, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. OgeuIscB AGC RNA

    Plasmid

    #222866

    Purpose

    This plasmid codes for the guide of the OgeuIscB with a AGC target

    Depositor

    Vincent Dion

    Article

    Mangin et al bioRxiv 2023.10.24.563270

    Insert

    OgeuIscB omega RNA with a (AGC)6 target sequence
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human U6

    Available since

    Oct. 16, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pWT055b

    Plasmid

    #96877

    Purpose

    theophylline-agRNA-FANCF

    Depositor

    David Liu

    Article

    Tang et al Nat Commun. 2017 Jun 28;8:15939. doi: 10

    Insert

    theophylline-agRNA-FANCF
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pUC57-ITR2-H1-sgAGC-SlugD10A-3XFLAG-SV40 NLS-ITR2

    Plasmid

    #222859

    Purpose

    This plasmid codes for the Slug-KH nickase with a sg(AGC)6

    Depositor

    Vincent Dion

    Article

    Mangin et al bioRxiv 2023.10.24.563270

    Insert

    Slug-KHD10A+sgAGC
    

    Use

    CRISPR

    Tags

    3X FLAG, SV40 NLS, ITR2 and ITR2

    Expression

    Mammalian

    Mutation

    KH variant and D10A mutation

    Promoter

    H1 and eCMV

    Available since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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